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QTL Mapping for Spike Length and Plant Height in ITMI Recombinant Intercross Lines Population of Wheat
YANG Lin, OU Jun, CHENG Yuan
jsau    2016, 34 (04): 397-401.   DOI: 10.16036/j.issn.1000-2650.2016.04.001
Abstract1306)      PDF(pc) (460KB)(1856)       Save
[Objective] The objective of this study was to identify QTLs for plant height and spike length based on a genetic map with high density and a RIL population. [Method] A recombinant intercross lines population contain 110 lines were used to perform QTL analysis based on a high density genetic map. [Results] A total of 12 QTLs for spike length were identified, and two of them were expressed in both investigated environments, and 6 were accounted for over 10% phenotypic variation. A total of 8 QTLs for plant height were identified, and 3 of them were expressed in both investigated environments, and two loci were accounted for over 10% phenotypic variation. [Conclusion] Taken together, All loci in present study are very important to spike morphology and yield improvement.
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jsau    2011, 29 (02): 141-298.  
Abstract1178)      PDF(pc) (19122KB)(1397)       Save
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Effect of Reducing Fertilizer Nitrogen on Quality of Kenjing 5 in Different Soil Fertility Level
JIANG Yu-wei, ZHAO Ting-ting, LIU Wen-wei, ZHONG Wei-jun, ZHANG Li-wei, ZHAO Hai-cheng, CHEN Li-qiang, NIU Tong-xu, LI Meng, ZHOU Jian, ZHENG Gui-ping
jsau    2016, 34 (04): 406-413.   DOI: 10.16036/j.issn.1000-2650.2016.04.003
Abstract694)      PDF(pc) (637KB)(1144)       Save
[Objective] In order to explore the effects of different soil fertility under the condition of reducing fertilizer nitrogen on quality of Kenjing 5. [Method] In present two-factor randomized block design was used to study the effects on reducing fertilizer nitrogen in rice(Kenjing 5) with different soil fertilizer(A1, A2, A3). [Results] Result indicated that reducing nitrogen fertilizer application can effectively improve grain processing quality with the increase of soil fertility. Under 174.0 kg/hm2 N of high soil fertility, 195.8 kg/hm2 N of middle soil fertility and 217.5 kg/hm2 N of low soil fertility condition, brown rice, milled rice rate and head rice rate had good quality; Increasing nitrogen fertilizer application appropriately could reduce chalkiness degree and percentage of chalky rice in low and middle soil fertility. Reducing nitrogen fertilizer application could decrease percentage of chalky rice and chalkiness degree in high soil fertility. Reducing nitrogen fertilizer in different soil fertility may increase amylose slightly and decrease protein content significantly, which could help to increase of the comprehensive evaluation of taste. [Conclusion] For Kenjing 5, there was significant difference in soil fertility and N application among appearance, nutritional quality and comprehensive evaluation of taste and their interations. Decreasing nitrogen application in high soil fertility and increasing nitrogen application low soil fertility was advantageous to improve processing and appearance quality of rice. Decreasing nitrogen fertilizer was beneficial to improve taste value in different soil fertility.
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Photosynthetic Characteristics of Lonicerae japonicae Flos New Variety “Huajin 6”
WANG Ling-na, SU Zheng, LIN Ying, ZHANG Yong-qing
jsau    2016, 34 (04): 426-430.   DOI: 10.16036/j.issn.1000-2650.2016.04.006
Abstract694)      PDF(pc) (475KB)(1057)       Save
[Objective] The aim of the study was to study the photosynthetic characteristics of Lonicerae japonicae Flos new variety "Huajin 6" and to provide references for reasonable cultivation measures. [Method] The light response curve, diurnal changes of photosynthetic parameters and chlorophyll fluorescence parameters changes of "Huajin 2" and "Huajin 6" were measured. [Results] Compared with "Huajin 2", "Huajin 6" had lower photosynthetic rate and stronger respiratory rate. Two varieties of Lonicerae japonicae Flos have "two peaks" type curve and two peaks appeared at 9:00 and 15:00, respectively. Typical phenomenon of "photosynthesis midday depression" appeared. Pn and Ls of two varieties exhibited similar dynamic, indicating that photosynthetic rate "photosynthesis midday depression" phenomenon may mainly attribute to the different of mesophyll cells carboxylation ability. Compared to "Huajin 2", the potential photosynthetic activity of "Huajin 6" was lower. It had no ability to resist strong light and high temperature. Under the same light intensity, the degree of photoinhibition was more serious than that of "Huajin 2". [Conclusion] The reasonable cultivation measures should be taken according to the variety of photosynthesis, transpiration, water use efficiency and chlorophyll fluorescence characteristics.
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Effects of Combined Application of Chemical Fertilizer Reduction with Trichoderma Organic Fertilizer on Yield and Quality of Pepper and Enzyme Activities in Rhizosphere Soil
JIN Yazhong, XIONG Yanan, SUN Xue, LI Jinyu, LI Xiang, HE Shuping, LI Chunxia
Journal of Sichuan Agricultural University    2021, 39 (2): 198-204.   DOI: 10.16036/j.issn.1000-2650.2021.02.009
Abstract184)      PDF(pc) (5576KB)(806)       Save
【Objective】 The effects of the reduced application of chemical fertilizers combined with Trichoderma organic fertilizer on yields and qualities of pepper and rhizosphere soil of pepper were explored. 【Method】 With no fertilization (CK) as the control, the effects of the reduced application of chemical fertilizers by 15%, 30% and 50% in combination with Trichoderma organic fertilizer on yields and qualities of pepper and enzyme activities in rhizosphere soil of pepper were investigated by plot experiments. 【Result】 Compared with CK treatment, all of fertilization treatments increased significantly the biomass and yield per plant and plot yield of pepper. Compared with 100% chemical fertilizer treatment (A), the biomass, yield per plant and plot yield of pepper treated with the reduced application of chemical fertilizer reduction by 30% and 50% in combination with Trichoderma organic fertilizer were not significantly different from 100% chemical fertilizer treatment. Whereas, the application of chemical fertilizer reduction combined with Trichoderma organic fertilizer significantly reduced the nitrate accumulation in pepper fruits, and the soluble sugar content of pepper fruits treated with the application of chemical fertilizer reduction combined with Trichoderma organic fertilizer at 45 days after flowering was markedly higher than that of 100% chemical fertilizer treatment. Combined application of chemical fertilizer reduction with Trichoderma organic fertilizer obviously increased the content of Vc in pepper fruits, while the soluble protein was visibly lower than that of 100% chemical fertilizer treatment (A). In addition, the activities of sucrase, polyphenol oxidase, dehydrogenase, urease, alkaline phosphatase and the contents of ammonium nitrogen, available phosphorus and available potassium in rhizosphere soil were remarkably increased by the treatments of 30% and 50% reduction of chemical fertilizer combined with Trichoderma organic fertilizer; nevertheless, the content of nitrate nitrogen in pepper fruits was decreased. 【Conclusion】 The reduced application of chemical fertilizer reduction combined with Trichoderma organic fertilizer from 30% to 50% can maintain pepper yield and improve fruit qualities, which may be related to the regulation of enzyme activities in rhizosphere soil, increasing of soil available nutrient contents and promoting the growth of pepper plants.
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Effect of Inorganic Salts, Vitamins and Phytohomones on Mycelial Growth of Laetiporus sulphureus
ZHANG Yuan-yuan, ZHANG Jing, WANG Yong, HAN Bao-kang, JIANG Xin-hua, ZHANG Bai-ren
jsau    2016, 34 (04): 488-492.   DOI: 10.16036/j.issn.1000-2650.2016.04.016
Abstract526)      PDF(pc) (334KB)(752)       Save
[Objective] In order to investigate the effects of inorganic salts, vitamins and phytohomones on the growth of Laetiporus sulphureus. [Method] Laetiporus sulphureus strain LS-1 was used as testing material and the mycelial growth was measured as index. Inorganic salts, vitamins and phytohomones were measured to obtain the effect of the mycelial growth on Laetiporus sulphureus. [Results] For different inorganic salts, magnesium sulphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride can promote mycelial growth of Laetiporus sulphureus, however, mycelial growth was inhibited by calcium chloride. When magnesium sulfate and monopotassium phosphate were 1.0 g/L, the mycelial growth reached maximal value(2.536 mm/d and 2.464 mm/d). Different types of vitamins VB2, VB4, VB6 had powerful enhancement on the mycelial growth of Laetiporus sulphureus. The mycelial growth reach a peak value result revealed as the concentration of VB4 was 10 mg/L. For different types of phytohomones, 6-KT can remarkably promote the mycelial growth of Laetiporus sulphureus followed by 6-BA. However, IAA and NAA had inhibition on the mycelial growth of Laetiporus sulphureus. [Conclusion] The results showed that different concentration of inorganic salts, vitamins and phytoho-mones had a great effect on the growth of Laetiporus sulphureus.
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Development of Novel Oligonucleotide Probes for Identifying Rye Chromosomes in Wheat Background
LIU Hong-kun, TANG Zong-xiang
jsau    2016, 34 (04): 402-405.   DOI: 10.16036/j.issn.1000-2650.2016.04.002
Abstract634)      PDF(pc) (293KB)(704)       Save
[Objective] Fluorescence in situ hybridization(FISH) technology can be used to identify rye chromosomes effectively in wheat backgrounds. Oligonucleotide probes and non-denaturing FISH(NDFISH) can increase the efficiency of detecting rye chromosomes. [Method] In this study, SLAF-seq(specific length amplified fragment sequencing)and bioinformatics methods were used to develop novel oligonucleotide probes. [Results] Three novel oligonucleotide probes Oligo-2874, Oligo-5296 and Oligo-9965 were developed and they can be used for ND-FISH assays. The three probes only hybridize to telomeres and sub-telomeres of rye chromosomes. [Conclusion] Therefore, these oligonucleotide probes can discriminate rye chromosomes in wheat backgrounds quickly and efficiently, and they enrich the probe sources for FISH analysis of wheat-rye hybrids. The results also indicate that SLAF-seq can be used to develop specific repetitive DNA probes for FISH analysis of relatives of wheat.
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Studies on Sand-fixing Plants Rhizosphere Microflora in Fixed Dune and Semifixed Dune in Erdos, Inner Mongolia
WANG Tian-long, ZHU Tian-hui, PIAO Chun-gen, WANG Lai-fa, LIU Zhao-xia
jsau    2007, 25 (03): 300-305.   DOI: 10.16036/j.issn.1000-2650.2007.03.021
Abstract80)      PDF(pc) (306KB)(700)       Save
This paper studies the aspect change of number and com position to rhizosphere soil microbes of Salix psammophila, Hedysarum mongolicum, Caragana korshinskii and Artemisia ordosica in fixed dune and semifixed dune, which are four typical sand-fixing plants in Erdos, Inner Mongolia. The results shows that the number of rhizosphere soil microbes in fixeddune and semifixed dune are aerophile bacteria > actinomyces > fungi. The number of aerophile bacteria was the most. The paper analyzed the change rule and cause. The results shows that the number of aerophile bacteria become uptrend in the evolvement process from semifixed dune to fixed dune. The number of rhizosphere Bacillus and endophytic bacteria change with seasons, and both reach to the largest in autumn.
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The Floristic Composition and Characteristics of Seed Plants in Cuona County, Tibet
CHEN Lin, Yang Guo-dong, LIN Guo-jun, YI Xian-gui, LI Long-na
jsau    2016, 34 (04): 431-439.   DOI: 10.16036/j.issn.1000-2650.2016.04.007
Abstract459)      PDF(pc) (576KB)(696)       Save
[Objective] This study aimed to investigate the composition and characteristics of seed plant flora in Cuona County, Tibet, providing information for the conservation of biodiversity and the sustainable utilization of plant resources in this region. [Method] Field surveys and historical records were used to analyze the floristic composition and resource characteristics of seed plants, following the methods of floristic geography and plant resources. [Results] ① In this region, we found 106 families, 416 genera with a total of 874 species, implying abundant plant resources of high values for medicine, horticulture, food and timber. ② At the family level, 23 dominant families(with at least 10 species for each) were identified, making up 66.83% and 73.91% of the total genera and species in this region, respectively. However, there was no dominant genus as most genera consisted of only one or a few species for each. ③ Pantropic and North Temperate were the dominant elements at the family level, while temperate elements dominated at the genus level with few endemic genera. ④ Cluster and principal analyses showed that the flora of Cuona County had a strong relationship with the flora of Shegyla Mountain, both of which were important components of the flora in eastern Himalaya. [Conclusion] The differences of temperate and tropical elements between family and genus level indicate the temperate elements may play an important role during the recent flora formation, consistent with the uplift of Qinghai-Tibet Plateau. The flora of Cuona County is an indispensable part of Tibetan flora, and it requires more attention for its abundant plant resources.
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FAN Gao-Qiong, YANG Wen-Yu, LIN Mo-Jun, ZHENG  Ting, YONG Ta-Wen, WANG Xiao-Chun, CHEN Meng-Xiang, BAI Wen-Jin
jsau    2009, 27 (2): 133-136.  
Abstract3127)            Save
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Anatomical Structures of Haloxylon ammodendron Flower and Its Adaptation to Desert Environment
SONG Yu-yang, ZHOU Chao-bin
jsau    2016, 34 (04): 420-425.   DOI: 10.16036/j.issn.1000-2650.2016.04.005
Abstract521)      PDF(pc) (622KB)(651)       Save
[Objective] The aim of the study was to explore the adaptation of Haloxylon ammodendron flower to arid environment in the Gurbantunggut desert of China. [Method] The anatomical structures of H. ammodendron flower were observed using paraffin section and scanning electron microscope and its pollen-ovule ratio was also measured. [Results] H. ammodendron flower attached at the new shoots(assimilating branches). The flower was bisexual and small, with a diameter of about 1.0 mm. The flower located at axil solitarily, only with calyx. The base of ovary positioned was within flower disk and the flower had no corolla. The flower had five tepals. The tepal was free, green, papery or scarious. The inner surface of the base was often arachnoid. The flower had five stamens and the stamens were yellow, which belonged to synantherous stamen. The solid style was short with many secretory cells and had no style in the center. The flower had two scaphoid bractlets, outside the cuticula. The bractlet had scarious margins. The ovary had vascular bundle and the connectivum of anther had vascular and the vascular preserves until anther dehiscence. When the flower was mature, the stigma was close to the base of anther. The anther height was slightly higher than that of the fingered stigma, providing a suitable spatial position to self-pollination. The flower had tiny and light but rich pollen grains. The P/O value was 64 815, which was suitable to anemophilous pollination. Based on the observations using scanning electron microscope, we found the pollen grain was nearly spherical with warty ornamentation. The length of polar axis and equatorial axis was 19.08~21.02 μm, 18.59~19.18 μm, respectively. [Conclusion] Our results indicated that H. ammodendron have two pollination ways(self-pollination and cross-pollination). The external feature and anatomical structure showed H. ammodendron flower has strong capacity to adapt to desert environment.
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Sequence analysis of the ITS region of nuclear ribosomal DNA (nrDNA) in Leymus duthiei and Leymus duthiei var. longearistatus
xing 0fan
jsau    2010, 28 (03): 265-270.  
Abstract1958)            Save

Sequencing of multiple clones of ribosomal ITS sequences in Leymus duthiei and Leymus duthiei var. longearistatus were performed to estimate ITS polymorphism within and between individual. Sequence diversity patterns, ITS RNA secondary structure and genealogical analysis suggested that (1) due to concerted evolution, ribosomal ITS sequences in L. duthiei and L. duthiei var. longearistatus may be biased towards the Ns genome donor; (2) concerted evolution might promote the ribosomal ITS sequences within L. duthiei var. longearistatus more homogenized than that within L. duthiei; (3) ITS sequence divergence between L. duthiei and L. duthiei var. longearistatus may be responsible for their geographic isolation.

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The Behavior Ethogram and PAE Coding System of Captive Bengal Slow Lorises
WANG Yu, XIE Meng, HE Xin, MENG Xiangyun, ZHANG Chen, GAO Yunxia, NI Qingyong
Journal of Sichuan Agricultural University    2019, 37 (06): 860-867.   DOI: 10.16036/j.issn.1000-2650.2019.06.016
Abstract187)      PDF(pc) (1128KB)(581)       Save
【Objective】 The objective of this study was to obtain the behavioral ethogram and its PAE coding system of the captive slow lorises. 【Method】 Behavioral data of captive Bengal slow lorises were collected by infrared monitoring cameras from November 2017 to October 2018 in GejiuBaohua Park Zoo,Prefecture of Honghe, Jinghong Nan-ken-he Rescue Center, Prefecture of Xishuangbanna, and Simao Tai-yang-he Rescue Center, Pu'er, Yunnan province. We used focal animal sampling and instantaneous scanning method to quantify the data and code them to follow the "posture-act-environment" (PAE) coding system. 【Result】 A total of 16 postures, 89 acts and 119 behaviors were distinguished and described. Based on the ecological function of behaviors, all of the recorded behaviors were divided into 12 categories, including feeding, foraging, eliminate, thermo-regulatory, abnormal, courtship, aggressive,affiliative, resting, locomotive, submissive and miscellaneous behaviors. 【Conclusion】 We found higher frequency of abnormal and stereotypic behavior in captive slow lorises, which indicated that environmental and food enrichment were not well-designed.
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Bird Resources and Community Diversity in Agricultural Area of Yingjing County
WEI Ding-ju, ZHAO Jun-song, LI Bo, ZHU Heng-da, CHENG Yong, NI Qing-yong
jsau    2016, 34 (04): 499-504,518.   DOI: 10.16036/j.issn.1000-2650.2016.04.018
Abstract289)      PDF(pc) (428KB)(580)       Save
[Objective] It is critical to make rational land use planning and decrease human disturbances for avian diversity conservation. [Method] We did investigation about farming area birds resources by an interview and line-transact survey in Yingjing County. [Results] A total of 219 bird species, belonging to 51 families and 16 orders were found in Yingjing County. Five species were listed as Class I protected animal species in China, and 17 species were listed as Class Ⅱ. Ten species were protected as provincial protected bird species in Sichuan. Six species were endemic to China. [Conclusion] Bird species diversity was higher in areas where topography and vegetation types were complicated. Birds of Passeriformes were dominant in agricultural area in Yingjing County.
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Studying Proceeding of Plant Potassium Nutrient
LI Ting-qiang, WANG Chang-quan
jsau    2001, 19 (03): 281-285.   DOI: 10.16036/j.issn.1000-2650.2001.03.025
Abstract133)      PDF(pc) (231KB)(554)       Save
The study of plant potassium nutrient is divided into three stages. Five aspect researches, e.g. the physiological function of potassium, the effect of potassium on the yield and quality of agricultural products, adverse circumstance force and potassium nutrient, bio-potassium fertilizer and the choosing of genes which produce low content potassium are reviewed in detail. The study proceeding of potassium in our country in recently years is also discussed. Meanwhile the study way and focal point about potassium have been put forward. Studying and making bio-potassium fertilizer and choosing the genes which produce low content potassium is to be the stress and the effective way to solve the short potassium resource in our country in the future.
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Effects of Root Exudates of Tall Fescue on Degradation of Soil Organochlorine Pesticides
PAN Sheng-wang, LEI Zhi-hua, HE Mao-ping, WU Yun-xiao
jsau    2016, 34 (04): 471-477.   DOI: 10.16036/j.issn.1000-2650.2016.04.013
Abstract394)      PDF(pc) (774KB)(545)       Save
[Objective] The objective of this study was to clarify the effects of root exudates on degradation of organochlorine pesticides(OCPs)in soils and to evaluate microbial ecological characteristics in OCPs-contaminated soils. [Method] Pot experiments were carried out to investigate the effects of root exudates of tall fescue(Festuca arundinacea) on degradation of total amount of OCPs and its main components in soils by methods of GC-ECD and GC/MS-MS. To determine influence of root exudates on the rhizosphere microbial growth and their community structure, soil microbial biomass carbon(SMBC)and their phospholipid fatty acids(PLFA) in soils were also analyzed by TOC-500 analyzer and GC-MS, respectively. [Result] The root exudates of tall fescue significantly enhanced the removal of OCPs. The removal rate was significantly higher in the treatment(77.57%) than in the control(33.49%). Under the same treatment conditions, the enhanced removal rates of hexachlorocyclohexane(HCHs), toxaphene, hexachlorobenzene(HCB), aldrin and γ-chlordane were much higher than one of the total amount of OCPs while the extent of enhanced dissipation of dichloro-diphenyl-trichloroethanes(DDTs), mirex, endosulfanⅠ, dieldrin and heptachlor epoxide were always lower than that in corresponding soils. Soil microbial biomass carbon increased with increasing pyrene concentration and there were close relationship between OCPs degradation and soil microbes during the whole experiment. Microbial community was dominated by bacteria, followed by fungi. They had the similar variation trend along with OCPs degradation, indicating that the OCPs in soils were degraded mainly by bacteria and fungi. Therefore, root exudates may influence the OCPs degradation through altering bacterial and fungi population. [Conclusion] Root exudates of tall fescue might change soil microbial community and structure during the process of phytoremediation, and further modify the degradation of OCPs.
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Water-holding Capacity of Four Different Types of Sphagnum Wetlands in the Tianbaoyan National Nature Reserve
JIAN Li-yan, XIAO Shi-hong, ZHANG Zhong-rui, HE Dong-jin, HONG Wei, LIU Jin-shan, CAI Chang-tang
jsau    2016, 34 (04): 414-419,425.   DOI: 10.16036/j.issn.1000-2650.2016.04.004
Abstract350)      PDF(pc) (483KB)(531)       Save
[Objective] The aim of the study was to clarify the water-holding capacity of soils and litters of 4 different Sphagnum wetlands(Palhinhaea cernua, Pllostachys heteroclada, Juncus effuses and Sphagnum magellanicum) in the Tianbaoyan National Nature Reserve in Fujian Province and to provide theoretical basis for protection and utilization of Sphagnum wetlands in the area. [Methods] Four different types of Sphagnum wetlands were used in this study. Soil and litter samples were collected to measure soil chemical properties, water-holding capacity characteristics. [Results] The soil bulk density of the 4 types of Sphagnum wetlands followed an order of P. cernua > S. magellanicum > P. heteroclada > J. effuses. The total porosity of soil was in the order of P. heteroclada > S. magellanicum > J. effuses > P. cernua. The soil water storage was followed the pattern of P. heteroclada > S. magellanicum > P. cernua > J. effuses. Litter water-holding capacity had a visible logarithmic correlation with immersion time and there existed a significant function relationship between water absorption rate and immersion time. The natural waterholding capacity, natural water-holding rate, maxima water-holding capacity, maxima water-holding rate of litter were 5.5~16.1 t/hm2, 31.8%~47.2%, 20.1~198.4 t/hm2 and 330.0%~805.5%, respectively. [Conclusion] The comprehensive analysis of water-holding capacity of 4 different types of Sphagnum wetlands indicated that S. magellanicum type has a relatively high water-holding capacity and is more beneficial to water conservation.
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WANG Xiao-Chun, YANG Wen-Yu, ZHANG  Chao, CHENG Yuan-Shuai
jsau    2009, 27 (2): 153-156.  
Abstract2848)            Save
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Research on the Degree of Use and the Influencing Factors of Farmers' Machinery——Based on the Survey of 205 Households of Kiwifruit Farmers in Sichuan Province
REN Dan, QI Yan-bin, YU Wei-yong, HE Yue, DENG Xin, FU Li
jsau    2016, 34 (04): 528-534.   DOI: 10.16036/j.issn.1000-2650.2016.04.022
Abstract441)      PDF(pc) (376KB)(520)       Save
[Objective] In order to deepen the understanding of agricultural machinery use and the influence of social capital on it. [Method] Based on the agricultural machinery using data of 205 kiwi fruit growers in Sichuan province, building the social capital measurement system, using the ordered logistic model, deeply analyzed the degree of farmers' machinery use and its influencing factors. [Results] The result shows that ① farmers' mechanization degree is generally low, and the popularity of agricultural machinery could be largely promoted. ② proportion of surnames among farmers and the number of farmers' relatives serve as local leaders have significant positive relation with farmers' use of machinery. ③ Farmers who participate in agricultural technology association are more likely to use agricultural machinery than those not. ④ Farmers who get relative certificates and who manage farms of lager scale are more likely to use agricultural machinery. [Conclusion] To promote agricultural mechanization development, Sichuan province could strengthen agricultural mechanization and develop local technical training, cultivate new professional farmers, standardize the land circulation system, and guide farmers in moderate-scale management.
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CHEN Li-Jing, BO Gang-Gang, HE  Jing, LIU  Jun, ZHANG Li-Jun, ZHANG  Li
jsau    2009, 27 (2): 148-152.  
Abstract3564)            Save
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The Mechanism for Developing Pork Quality Traits
CHEN Dai-wen, ZHANG Ke-ying, HU Zu-yu
jsau    2002, 20 (01): 60-66.   DOI: 10.16036/j.issn.1000-2650.2002.01.015
Abstract122)      PDF(pc) (322KB)(518)       Save
The chemical compositions and nutrient metabolism are the biochemical basis for the development of pork quality traits. Pork color is dependent upon the oxidation reduction status and the amount of oxygen bound. Pork tenderness is related to working condition of myofibrils, the content and constitutes of connective tissue, intramuscular fat content and protein-breaking enzymes. Pork flavor is associated with flavoring substances and intramuscular lipids in the meat. Boar taint originates from androstenone and skatole. Pork color, flavor and tenderness are all influenced by pork water-holding capacity, which is affected by muscle pH and lipid oxidation. All the above aspects have been reviewed in this article.
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Cladistic Analysis of 8 Species of Iris Based on Morphological Characters
YU Xiao-fang, JIANG Yu-lin, LIU Yu-jing, FENG Yi-mei, ZHOU Yong-hong
jsau    2016, 34 (04): 440-444.   DOI: 10.16036/j.issn.1000-2650.2016.04.008
Abstract378)      PDF(pc) (360KB)(503)       Save
[Objective] The aim of the study was to analyzed the 27 morphological characters of 8 species and 1 varietas in Iris and to provide morphological basis for interspecific classification relation-ships of genus Iris. [Method] On the basis of numerical taxonomy study of Iris L. , 27 typical morphologi-cal traits of 8 species and 1 varietas of Iris were coded. The Belamcanda chinensis(L. ) DC was taken as outgroup and their MP phylogenetic tree was constructed. [Results]I. lactea var. chinensis and I. lactea formed A clade and the bootstrap support was 96%. ② I. leptophylla, I. sichuanensis and I. go-niocarpa formed B clade and the bootstrap support was 55%. However, I. leptophylla, I. sichuanensis consisted one little clade from I. goniocarpa and the bootstrap support was 83%. ③ I. japonica, I. wattii and I. confusa comprised the C clade the bootstrap support was 57%. Nevertheless, I. wattii and I. confusa formed one little clade from I. japonica and the bootstrap support was 78%. [Conclusion] ① The relationship of I. lactea var. chinensis and I. lactea was close, supporting the hypotheisis that I. lactea var. chinensis is a variety of I. lactea. ② The relationship between I. leptophylla and I. sichuanensis was closer as compared to I. goniocarpa. ③ I. japonica had relatively distant relationship with I. wattii compared to I. confusa.
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Spatial and Temporal Variations of Absolute Humidity in Guizhou Province from 1960 to 2013
HAN Hui-qing, CAI Guang-peng, WU Jing, ZHOU Shan
jsau    2016, 34 (03): 348-353.   DOI: 10.16036/j.issn.1000-2650.2016.03.015
Abstract251)      PDF(pc) (864KB)(502)       Save
[Objective] The aim of the study was to explore the variations of absolute humidity in Guizhou Province. [Method] Based on the weather observation data from 1960 to 2013, the temporal and spatial variations of absolute humidity and its relationship with precipitation in Guizhou province were analyzed using the method of absolute humidity calculation. [Results] The absolute humidity declined in Guizhou province over the period. Absolute humidity was maximum in summer, followed by spring and autumn, and was minimal in winter. Absolute humidity in the east and south parts of study area were higher than the west part. Absolute humidity was higher in basin, hill, low and moderate mountains than in the plateau. There were differences in each landform for absolute humidity among different seasons. Absolute humidity fluctuated with the increase of slope but declined with the increase of elevation. A positive correlation between absolute humidity and precipitation was found. There were consistencies in spatial pattern, proportion of season and topography between absolute humidity and precipitation. [Conclusion] Spatial and temporal variations of precipitation are dominant factors affecting variation of absolute humidity in Guizhou province.
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Effect of Leptin on the Proliferation and Differentiation of Chicken Primary Preadipocytes
QIN Fei, ZHANG Yu, LIU Jia, ZHU Qing, WANG Yan, YIN Hua-dong, LI Di-yan, ZHAO Xiao-ling
jsau    2016, 34 (04): 505-510.   DOI: 10.16036/j.issn.1000-2650.2016.04.019
Abstract352)      PDF(pc) (467KB)(499)       Save
[Objective] In order to explore the effects of leptin on the proliferation and differentiation of chicken primary preadipocytes. [Methods] Separated from 12-day-old Cobb broilers abdominal fat for cell culture, cells were treated with 0, 50, 200, 800 ng/mL leptin after being plated for 24 h, respectively. Culture liquids were changed per 24 h time interval until 72 h. Growth curves for the four experimental groups were draw via CCK-8 method. The determination of fat content was done via oil red O extraction method. Expressions of key genes PPARγ and SREBP1c during the differentiation of preadipocytes were detected via q-PCR. [Results] The results of CCK-8 method and oil red O extraction indicated that 0~800 ng/mL leptin had no significant effect on the proliferation and differentiation of chicken preadipocytes(P>0.05), meanwhile the Liptin had no significant effect on the expression of PPARγ and SREBP1c(P>0.05), respectively. [Conclusion] The study proved that leptin had no effect on the proliferation and differentiation of chicken preadipocytes.
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Impacts of litter on soil microbe and nutrient in soils of natural evergreen broadleaved forest after artificial regeneration
Wang JingYan
jsau    2010, 28 (03): 285-290.  
Abstract2377)            Save

Soil bacteria, fungi and actinomycetes counts, and hydrolysis N, available P and K contents in soils were determined to understand the effects of litter on soils in natural evergreen broadleaved forest, Sassafras tzumu plantation and Cryptomeria fortunei plantation. Soils were collected in the 0~20 cm layer and incubated for 15, 30, 45, 60, 75 and 90 days in the laboratory at 25 ℃ with natural water content and different litters. The results indicated that microbial counts increased and decreased with incubation time pre- and post- reached the max values, respectively. Bacteria counts reached the max value in 45 and 60 days incubation in soils with and without forest litter addition, respectively; fungi and actinomycetes counts reached the max value in 60 and 45 days incubation, respectively. Soil nutrient contents increased continuously with incubation time. Microbial counts and nutrient contents in soils showed the ranked order of natural evergreen broadleaved forest > Sassafras tzumu plantation > Cryptomeria fortunei plantation when the forest litter was not added to the soil. The added forest litter increased the microbial counts and nutrient contents in soils regardless of the forests. Added the litter from the natural evergreen broadleaved forest in soils of the Sassafras tzumu plantation and Cryptomeria fortunei plantation led to higher microbial counts and nutrient contents, as compared with added the litter from the Sassafras tzumu plantation and Cryptomeria fortunei plantation, respectively. There were close relationships between soil microbial counts and nutrient contents. The results suggested that the artificial regeneration of the natural evergreen broadleaved forest into the Sassafras tzumu plantation and Cryptomeria fortunei plantation might lead to the decrease of soil microbial counts and nutrient contents; in addition, the composition and quality of forest litter have important impacts on soil fertility.

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Genealogical analysis of the rbcL sequences of Kengyilia (Triticeae: Poaceae) species from the Qinghai-Tibetan Plateau
xing 0fan
jsau    2010, 28 (03): 271-275.  
Abstract1461)            Save

To estimate phylogenetic relationships of the Qinghai-Tibetan Plateau Kengyilia species and the maternal donor during its speciation events, chloroplast rbcL sequence were analyzed for nine Kengyilia taxa, together with two Pseudoroegneria (St) and two Agropyron (P) taxa. Genetic and genealogical analysis suggested that: (1) there is a close relationship among Kengyilia stenachyra, Kengyilia kokonorica, Kengyilia hirsute, Kengyilia rigidula and Kengyilia grandiglumis; (2) the rbcL sequences of Kengyilia stenachyra and Kengyilia geminate are evolutionarily distinct; (3) both Pseudoroegneria and Agropyron could serve as the maternal donor during the speciation of allotetraploid Kengyilia speices from the Qinghai-Tibetan Plateau.

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ZHOU Wei-Na, JIANG Yuan-Qing
jsau    2009, 27 (2): 157-161.  
Abstract2653)            Save
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Research on Rural Ecotourism Planning Under the Background of Reconstruction after Quake in Sichuan——-Citing the Ecotourism Planning of Penghua Village In Zundao Town of Mianzhu City as a Case
Cai Jun
jsau    2010, 28 (03): 319-323.  
Abstract1835)            Save

In recent years, rural eco-tourism is booming in Sichuan, which has been a kind of major tourism industry. “5.12” Wenchuan earthquake made it suffered heavy losses, which caused massive destruction to the rural natural environment, eco-tourism resources, tourism service facilities and the residents community, meanwhile seriously hampered the development of local rural eco-tourism. Therefore, the reconstruction planning of Sichuan rural eco-tourism after Earthquake is an important part of “restoring life, developing production and resorting ecology”. In this paper, new problems about Sichuan rural eco-tourism after Earthquake were proposed to analyze new opportunities about the rural eco-tourism development reconstruction after Earthquake. Sichuan rural eco-tourism planning of reconstruction after Earthquake was studied by the way of taking Eco-tourism planning in Penghua Village, Zundao Town in Mianzhu City as an example.

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343
jsau    2009, 27 (2): 3-5.  
Abstract1261)            Save
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Antibacterial Activity of Essential Components of Cinnamomum longepaniculatum Oil on Several Typical Pathogenic Bacteria
ZHANG Ping, WANG Ping, SHI Chao-feng, WEI Qin, DU Yong-hua, ZHOU Li-jun, YIN Zhong-qiong, JIA Ren-yong
jsau    2013, 31 (04): 393-397.   DOI: 10.3969/j.issn.1000-2650.2013.04.006
Abstract1176)      PDF(pc) (559KB)(474)       Save
[Objective] The aim of the study was to measure the antibacterial activity of essential components of Cinnamomum longepaniculatum oils,such as 1,8-cineole (99.5%),terpinene-4-alcohol (99%) and γ-terpinene (99%).[Method] Several typical pathogenic bacteria such as Salmonella enteritidis (Salmonella),Escherichia coli and Staphylococcus aureus (S. aureus) were used to determine the minimal inhibitory concentration and minimal bactericidal concentration,the growth curves were drawn using CFU dilution-plate method.[Results] The terpinene-4-alcohol exhibited the highest antibacterial activity to tested bacteria,followed by 1,8-cineole.γ-terpinene was effective to Salmonella,but ineffectual to E.coli and S. aureus.[Conclusion] The antibacterial activity of 3components of C. longepaniculatumoil all were dependent on time and concentration.
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YONG Ta-Wen, YANG Wen-Yu, WANG Xiao-Chun, FAN Gao-Qiong
jsau    2009, 27 (2): 167-.  
Abstract2272)            Save
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Cloning of Plus-trees from Sichuan Native Poplars and Evaluating its Growth at Seedling Stage
jsau    2010, 28 (03): 302-305.  
Abstract2475)            Save

Cloning of plus-trees selected from Sichuan native poplars and evaluating its growth at seedling stage were carried out on the basis of investigation, selection and collecting poplar resources in Sichuan. The results indicated that the biomasses of one-year-old cutting seedlings were remarkably different among the different poplar clones. Clones of hybrid named CNO727, CN0728 and CN0729, P. yunnanensis, P. schneideri, P. schneideri var. tibetica, P. trinervis grew well and were adaptive to the low altitude in Sichuan, so it is relative feasible to select improved varieties. P. gonggaensis was one of rare poplar resources and was used for garden greening. The others poplar clones grew weakly and showed bad adaptability for the natural ecological conditions of the low altitude in Sichuan, so it is impossible to direct use them.

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Effect of Different Tillage Treatment on the Accumulation of Dry Matter and Yield of Maize in the Middle of Sichuan Hilly Basin
WU YongCheng
jsau    2010, 28 (03): 276-279.  
Abstract2538)            Save

In this field experiment,single radom-plot design was conducted to study the effect of six tillage treatments including T1(flat-planting),T2(flat-planting with straw mulching),T3(flat-planting with plastic film mulching),T4(straight bed-planting with plastic film mulching),T5(planting on the side of plastic film mulching),T6(bed-planting cross slope)on yield and dry matter accumulation of maize c. v. zheng-hong311 under the condition of natural drought in the Middle of Sichuan Hilly Basin. The results showed that the treatments with plastic film mulching(T3、T4、T5)had high dry matter accumulation and grain weight relatively. Furthermore, the plant dry matter, stem and leaf transport value and its contribution rates to grain of T4、T5 treatments were significantly higher than T1、T2、T6 treatments. All the treatments with plastic film mulching(T3、T4、T5)have little difference of grain yield , but they were significantly higher than T1、T2、T6 treatments. Based on the condieration of simplified cultivation, T3 and T5 treatments are better tillage way in spring maize.

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Effects of Different Spatial Resolution on LAIe Estimation of Evergreen Broad-leaved Forests in Southwest Sichuan
ZHAO An-jiu, CHEN Yin-hua, MAO Jia-yong
jsau    2016, 34 (04): 456-463.   DOI: 10.16036/j.issn.1000-2650.2016.04.011
Abstract386)      PDF(pc) (582KB)(460)       Save
[Objective] The aim of the study was to analyze the effects of different spatial resolutions on the LAIe estimation. [Method] Effective leaf area index(LAIe) of montane evergreen broad-leaved forests in southwest Sichuan was inventoried and assessed based on 83 field plots of 20 m×20 m and spectral vegetation index(Normalised Difference Vegetation Index, NDVI; Soil Adjusted Vegetation Index, SAVI; Ratio Vegetation Index, RVI) were extracted from different spatial resolution image data of Landsat-8(30 m), SPOT-5(10 m), Pleiades-1(2 m). Based on remotely sensed data, inventoried LAIe and auxiliary variables(eg. relief data), regress models were established by partial least multiplicative regression method. [Results] NDVI and SAVI extracted from three kinds of resolution remote sensing data were most important to LAIe estimation. The VIP value of NDVI and SAVI extracted from Landsat-8 was 1.662. The VIP value of NDVI, and SAVI extracted from SPOT-5 was 1.573. Similar to Pleiades-1, the VIP value was 1.423. According to three kinds of sensors, all correlation coefficients between NDVI, SAVI and LAIe were more than 0.8, all of which were significant. The coefficient of determination R2 was 0.793 and precision accuracy was 79.8% for the regress model using Landsat-8 data. Likewise, the coefficient of determination R2 was 0.853 and precision accuracy was 84.4% for the regress model using SPOT-5 data. Moreover, R2 and P of regress model using Pleiades-1 data was highest compared to other regress models, which reached to 0.898 and 89.5%, respectively. [Conclusion] Different image spatial resolution have significant effects on LAIe estimation and the performance of LAIe estimation can be improved significantly using high spatial resolution optical data, such as Pleiades-1 remote sensing data.
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Screening and Compounding of Biocontrol Bacillus and Fungicide and Their Control Effect on Branch Withered Disease on Koelreuteria paniculata
ZENG Yanling, LIU Han, LI Zhaojia, LI Shujiang
Journal of Sichuan Agricultural University    2021, 39 (1): 55-62.   DOI: 10.16036/j.issn.1000-2650.2021.01.009
Abstract320)      PDF(pc) (4782KB)(459)       Save
【Objective】 The aim of this study was to improve the effect of biocontrol Bacillus on the control of branch withered disease on Koelreuteria paniculata,reduce the amount of chemical agents used,and alleviate the problem of chemical resistance,and to explore the compound control effect of biocontrol Bacillus and fungicide.【Method】 The biocontrol Bacillus was screened by plating method,the fungicide was screened by the growth rate method.The biocompatibility of biocontrol Bacillus and fungicide was determined by spectrophotometer,and the compound ratio was determined by Wadley method.【Result】 One Bacillus amyloliquefaciens strain was screened with the strongest antagonistic effect on Neofusicoccum parvum; the EC50 value of carbendazim 50% WP was the lowest,which was 0.123 mg/L,and it had biocompatibility with Bacillus amyloliquefaciens.When the volume ratio of carbendazim 50% to Bacillus amyloliquefaciens was 5 : 1,the EC50 (ob) value was 0.039 mg/L,and the SR value was 3.783.The control effect of the compound agent on branch withered disease of Koelreuteria paniculata was 83.83%.【Conclusion】 The control effect of carbendazim combined with Bacillus amyloliquefaciens on branch withered disease of K.paniculata was significantly higher than that of single agent.The application of compoud agent could reduce the use of chemical fungicides.
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LIN Mo-Jun, WU Ju-Xian, LEI Ting-Qi, TUN Jin-Xiu, YANG Wen-Yu, BANG  Hu
jsau    2009, 27 (2): 162-166.  
Abstract2387)            Save
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DNA Extraction and Wood ISSR Identification from Three Phoebe Species
WANG Ying, LUO Jia-yan, ZHONG Wen-han, SHI Jiang-tao, FAN Shuai
jsau    2016, 34 (04): 450-455,477.   DOI: 10.16036/j.issn.1000-2650.2016.04.010
Abstract404)      PDF(pc) (640KB)(451)       Save
[Objective] The aim of the study was to identifying 3 phoebe species by inter simple sequence repeat(ISSR) technique. [Method] The optimized CTAB-SDS, CTAB and kit for DNA extraction methods were used to extract wood DNA from P. sheareri, P. chekiangensis and P. zhennan. Three species were amplified with ISSR-PCR technique. [Results] The optimized CTAB-SDS and CTAB methods were better for DNA extraction of 3 woods. 7 ISSR primers were screened and their amplification were stable, clear and high polymorphic. A total of 67 bands were obtained through 7 primers, 43, 64.2% of which were polymorphic. DNA fragment size were ranged from 100 bp to 2 000 bp. [Conclusion] These polymorphism bands amplified by the 7 primers can distinguish the woods of 3 species.
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