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Genetic Diversity Analysis of Cunninghamia lanceolata in the 2nd and 2.5th Generation Seed Orchard
FANG Yue, YANG Hanbo, WU Huaxue, LEI Xun, ZHANG Xiaoguo, YANG Changtong, HUANG Zhen, CHEN Lianghua, ZHU Peng
Journal of Sichuan Agricultural University    2022, 40 (3): 371-378.   DOI: 10.16036/j.issn.1000-2650.202202040
Abstract855)      PDF(pc) (4991KB)(53)       Save
【Objective】 Understanding the genetic background of the 2nd and 2.5th generation seed orchards of Cunninghamia lanceolata in Hongya Forest Farm to provide the basis for the production and genentical management of these seed orchards and for the construction of the 3rd generation seed orchards of C. lanceolata. 【Method】 In this study, 13 SSR molecular markers were used to analyze the genetic diversity and genetic structure of the 2nd and 2.5th generation seed orchards of C. lanceolata in Hongya Forest Farm. 【Result】 ①The values of Na(10.1), Ne(4.71) and I(1.213) in this 2nd generation seed orchards were basically higher than most of the seed orchards or breeding resources of C. lanceolata and other coniferous species, while values of Ho(0.440) and He(0.478) were lower than those seed orchards or breeding resources. However, the index of genetic diversity of a single locus were similar to those in the studies with the same loci. ②Although the value of Na in the 2nd generation seed orchard was decreased somewhat than that in the 2.5th generation seed orchard, the other index of genetic diversity did not decrease significantly. ③ The genetic diversity among the sources were different somewhat, yet the genetic differentiation was not obvious. The values of gene flow were greater than 1(implying a frequent gene exchange among the sources), the genetic variation was mainly existed among the sources(accounting for 99%), and no obvious genetic clustering of all the 110 clones of the 2nd generation orchard was also not found. 【Conclusion】 The 2nd generation seed orchard of C. lanceolata in State-Owned Forestry Farm of Hongya has rich genetic diversity, and the genetic diversity of the 2.5th generation seed orchard did not decrease significantly as well, which could guarantee the seed quality and genetic gain in the future. Since the genetic structure of the mother tree clones in the seed orchard is relatively consistent, some good genetic materials from other geographical provenances or ancient tree resources of C. lanceolata should be supplemented to increase the genetic differentiation to improve the genetic gain of the seed orchard during the construction of the 3rd generation seed orchard.
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Population Structure and Dynamic Characteristics of Taxus wallichiana var. mairei in Liancheng County, Fujian Province
XIE Chunping, SHEN Shunting, LIU Dawei, YU Xiaoya, HUANG Shaohu
Journal of Sichuan Agricultural University    2022, 40 (3): 379-386.   DOI: 10.16036/j.issn.1000-2650.202204072
Abstract733)      PDF(pc) (1452KB)(102)       Save
【Objective】 The old trees of Taxus wallichiana var. mairei are mostly scattered in southern China, and it is difficult to form populations with a certain number of individuals. The analysis of the population structure and dynamics of the old trees can provide a scientific basis for the restoration and the cultivation of old tree resources. 【Method】 In this study, the population structure and dynamics of two old trees populations of T. wallichiana var. mairei in Liancheng County of Fujian Province were analyzed by drawing a diagram of the diameter class structure and survival curves, compiling static life tables, and calculating population dynamic change indices. 【Result】 ①The individuals of Ⅰ-diameter class of both old tree populations were small. The population in Guodi Village, Laiyuan was a declining population, while the other is a weak growth population in Baishi Village, Quxi. ②Static life tables showed that the viability and life expectancy of two populations began to decline after reaching the intermediate diameter class. ③ The population survival curve of Guodi Village in Laiyuan was the b1 variant of Deevey-Ⅱ, and that of Baishi Village in Quxi was Deevey-Ⅲ. ④ The dynamic indices of population in the absence and presence of disturbance at two sites were -3.03 and 7.5, -0.34 and 0.75, respectively. This was consistent with the characteristics of population structure. 【Conclusion】 Reducing anthropogenic disturbance, enhancing seed germination rate and increasing the individual numbers of small diameter classes are the keys to promote the recovery of old tree populations of T. wallichiana var. mairei in this region.
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Genome-Wide Identification, Evolution and Expression Analysis of TCP Gene Family in Celery (Apium graveolens L.)
ZHOU Jin, LI Jie, LI Xiaoyan, XIAO Danyi, LI Mengyao
Journal of Sichuan Agricultural University    2022, 40 (2): 145-155.   DOI: 10.16036/j.issn.1000-2650.202110039
Abstract592)      PDF(pc) (14292KB)(439)       Save
【Objective】 The members of TCP transcription factor family in celery genome were screened and identified, which provided a theoretical basis for the functional research of TCP gene and genetic improvement of celery. 【Method】 In this study, the TCP family members were identified based on the whole-genome data of celery, and their physical and chemical properties, gene structure, chromosome distribution, phylogenetic evolution, protein functional domain, and transcript abundance were also analyzed. qRT-PCR was further performed to verify the expression under high temperature stress. 【Result】 The results showed that a total of 29 TCP genes were identified in celery, which were divided into two major classes: Class I and Class II. Most of the TCP genes are located on the chromosomes and are unevenly distributed, and only two genes are located on the contigs. All members contain conserved structural domains. Evolutionary analysis showed that TCP genes of Apiaceae crops displayed closer relationships. Promoter analysis showed that the promoter region of TCP family members was significantly enriched in cis-elements related to growth and development, hormone response and abiotic/biotic response. Expression pattern analysis showed that most genes of the TCP family are highly expressed in celery leaves, and the expression abundance in white, green and red celery is high, and the Class I subgroup may also be involved in the response to high temperature stress. 【Conclusion】 The research results are helpful in further understanding the systematic evolution and gene function of celery TCP family members, and also providing a certain theoretical basis for the genetic engineering improvement of celery.
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Research Progress on Wheat Spike Development-Related Genes
MA Jian, DING Puyang, WANG Surong, MOU Yang, TANG Huaping, TANG Liwei, LAN Xiujin
Journal of Sichuan Agricultural University    2022, 40 (1): 1-9.   DOI: 10.16036/j.issn.1000-2650.202110009
Abstract478)      PDF(pc) (1911KB)(389)       Save
【Objective】 To understand key genes and their regulation pathways associated with spike development in wheat,and thus lay a foundation for genetic improvement and breeding utilization of spike traits,we here reported on progress on wheat spike development-related genes. 【Method】 The research progress of wheat spike development process,related genes and important quantitative trait loci (QTL) were summarized and analyzed by consulting the relevant literature. 【Result】 Spike is an important agronomic trait which directly determines the yield. Spike development of wheat has always been the focus of research by breeders and molecular biologists. Benefiting from the research progress in rice and maize,as well as the development of sequencing technology and molecular biotechnology,numerous studies have reported that a few of genes play a key role in wheat spike development. Regulatory pathways associated with wheat spike development have been gradually identified. 【Conclusion】 Compared with rice,the study on spike development in wheat is relatively slow. But with the development of sequencing technology and transgenic technology,the cloning of spike regulation-related genes will be greatly accelerated.
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Cloning and Expression Analysis of BoaBKI1 Gene in Chinese Kale (Brassica oleracea var. alboglabra)
LIANG Sha, HUANG Wenli, LI Xiangxiang, WANG Yilin, ZHANG Chenlu, ZHANG Fen, SUN Bo
Journal of Sichuan Agricultural University    2022, 40 (2): 163-171.   DOI: 10.16036/j.issn.1000-2650.202112051
Abstract444)      PDF(pc) (34169KB)(92)       Save
【Objective】 This paper intends to clone BoaBKI1 gene in Chinese kale (Brassica oleracea var. alboglabra),and perform bioinformatics and spatio-temporal expression analysis on it. 【Method】 The total RNA of Chinese kale (Brassica oleracea var. alboglabra) was extracted by modified CTAB method and reverse transcribed into cDNA. The primers were designed to clone BoaBKI1 gene. The sequence analysis of BoaBKI1 gene was performed by bioinformatics method. Spatio-temporal expression analysis of BoaBKI1 gene was performed by semi-quantitative PCR. 【Result】 The BoaBKI1 gene was cloned in Chinese kale. Sequence analysis showed that its open reading frame was 1 026 bp,which encoded 341 amino acids;its theoretical molecular weight was 37.30 kD,the isoelectric point was 9.67,and the molecular formula was C1603H2586N476O534S7. The results of the homology sequence alignment and phylogenetic tree analysis showed that the BoaBKI1 gene was conserved during evolution and has a sequence identity of up to 99% with Cabbage and European rape,which all belongs to the Brasscia genus. Semi-quantitative PCR analysis results showed that among different developmental stages,the highest expression of BoaBKI1 was observed in the true leaf stage,and the lowest one was in the germinating seed stage. The highest expression level of BoaBKI1 in flower buds was observed in the petals,and the lowest was in the sepals. The expression in the open flowers was significantly higher in the pistils than in other organs. With the flower opening,the expression levels of BoaBKI1 gene increased in each flower tissues,and pistils and stamens showed the most up-regulated trends. 【Conclusion】 These findings can provide a theoretical basis for studying the function and regulatory mechanism of BoaBKI1 in Chinese kale.
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Food Security Capacity: Connotation Characteristics, Index Measurement and Promotion Path
WANG Ruifeng, LI Shuang, KONG Fanna
Journal of Sichuan Agricultural University    2022, 40 (3): 301-311.   DOI: 10.16036/j.issn.1000-2650.202203066
Abstract314)      PDF(pc) (1361KB)(141)       Save
【Objective】 Ensuring national food security is the bottom line of the “three rural” work. 【Method】 This paper takes the new national food security strategy as the theoretical framework,this paper defines the connotation characteristics of food security capacity,constructs the evaluation index system of food security capacity,and then based on the relevant statistical data of Heilongjiang Province from 2009 to 2019,measures the food security capacity and explores the configuration path to improve the food security capacity. 【Result】 The research shows that the ability of food security is composed of five dimensions: basic security ability,market regulation ability,production and supply ability,the ability to use international market resources and the development ability of agricultural modernization. For the food security guarantee capacity of Heilongjiang province,the basic guarantee capacity,the ability to use international market resources and the development capacity of agricultural modernization all show an increasing trend,the production and supplication capacity shows a fluctuating upward trend,while the market regulation capacity shows a U-shaped trend of first decreasing and then improving. 【Conclusion】 Therefore,in order to improve the ability of food security,we need to strengthen the institutional guarantee by improving the ability of food security in the main grain producing areas,and establish and improve the long-term mechanism,coordination mechanism and emergency mechanism.
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Research on Influence Factors of Online Purchase Intention toward Fresh Agricultural Products Based on Social Cognition and Perceived Value
ZHAO Lei
Journal of Sichuan Agricultural University    2022, 40 (1): 137-144.   DOI: 10.16036/j.issn.1000-2650.201909006
Abstract252)      PDF(pc) (5435KB)(279)       Save
【Objective】 To explore the factors which influence the consumers' online shopping intention for fresh agricultural products, and provide new ideas for the electronic commerce development of fresh agricultural products. 【Method】 Based on the social cognitive theory and perceived value, builded a theoretical model, designed indicators from three dimensions of the consumer cognition, electronic commerce platform environment, production environment, and two levels of perceived benefits and perceived risks, and analyzed the factors which influence the online shopping intention for fresh agricultural products and the influence degree. 【Result】 The level of consumption of consumer cognition, the communication quality and after-sale quality of electronic commerce platform environment, the product quality, price and environment logistics of production environment, have a positive influence on perceived benefits. The knowledge level of consumer cognition, the website reputation, communication quality and after-sale quality of electronic commerce platform environment, the product quality and price of production environment, have a negative influence on perceived risks. Perceived benefits have a positive effect on online shopping intention for fresh agricultural products. Perceived risks have a negative effect on online shopping intention for fresh agricultural products. And the impact of perceived benefits is more significant than perceived risks. 【Conclusion】 The electronic commerce platform should enhance customers' perceived value and improve their online shopping intention, which need combine with the feature of fresh agricultural products.
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Processing Quality of Wheat Samples Harvested from Sichuan Province
QI Pengfei, LI Qingcheng, CHEN Qing, GUO Zhenru, ZHOU Caiyi, CHEN Chen, WANG Yan, KONG Li, WEI Yuming, ZHENG Youliang
Journal of Sichuan Agricultural University    2021, 39 (6): 705-712.   DOI: 10.16036/j.issn.1000-2650.2021.06.001
Abstract247)      PDF(pc) (4864KB)(226)       Save
【Objective】 To clarify the present situation of wheat processing quality in Sichuan province and provide key information for quality breeding. 【Method】 Seven wheat cultivars with different levels of gluten strength were planted in 18 ecological sites that can represent the current wheat producing regions in Sichuan province, in three consecutive wheat growing seasons. The harvested wheat samples were carefully cleaned and stored at room temperature before measuring processing quality parameters.【Result】 The processing quality parameters of the most harvested samples met the national standard for weak-gluten wheat, although these parameters varied among ecological sites, growing seasons and cultivars. When analyzing the data of each ecological site separately, we found that most areas of wheat producing regions in Sichuan (especially Chengdu Plain and north Sichuan) were suitable for the production of weak-gluten wheat. Notably, the samples of elite cultivars shown better weak-gluten quality, compared with those of other cultivars. Analysis of variance (ANOVA) indicated that the effects of cultivars, growing seasons and ecological sites on processing quality ranked as growing seasons>cultivars>ecological sites, under our experimental conditions, which further indicated that Sichuan is suitable for producing of weak-gluten wheat. To evaluate the end-use quality of the harvested wheat samples, the flour was used to make crisp cookies, and 20 brands of popular commercial cookie flour in the supermarket were used as control. With the exception of the cultivar "Shumai969", the flour of other 6 cultivars exhibited better cookie-making quality than control. When considering the difference in ecological sites, the flour in 17 of the 18 sites (except Zizhong County in Neijiang) shown better cookie-making quality than control. 【Conclusion】 The wheat planting region in Sichuan is one of the best place in China to produce high quality weak-gluten wheat, and the growing of elite cultivars in favourable areas is more conducive to the play of ecological advantages of Sichuan.
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Screening of Wheat Cultivars with High Nitrogen Efficiency at Seedling Stage and Construction of Index System in Southwest Wheat Region
ZHANG Heng, CHEN Yanqi, REN Jieying, YANG Hongkun, FAN Gaoqiong
Journal of Sichuan Agricultural University    2022, 40 (1): 10-18.   DOI: 10.16036/j.issn.1000-2650.202106013
Abstract235)      PDF(pc) (3864KB)(226)       Save
【Objective】 The differences of nitrogen absorption and utilization of different wheat genotypes in southwest wheat region at seedling stage were evaluated,the evaluation index system of nitrogen efficient wheat cultivars at seedling stage was explored,and nitrogen efficient wheat cultivars were selected. 【Method】 34 wheat cultivars in Southwest China were used as materials To analyze the differences in nitrogen efficiency related traits of wheat cultivars at seedling stage under high nitrogen (15 mmol/L) and low nitrogen (2.5 mmol/L) levels. The comprehensive evaluation was carried out by membership function method and systematic cluster analysis. 【Result】 There were significant differences in the physiological morphological traits of wheat cultivars at seedling stage under the two N treatments. Among the 10 evaluation indexes,the variation coefficients of RSR,LA,TNA,SPAD,SRA,RDW and SDW were all higher than 20.5% under the two nitrogen treatment levels. It can be used as an efficient nitrogen screening index at wheat seedling stage. At the same time,a comprehensive evaluation method combining membership function method and systematic cluster analysis was established,and four cultivars,such as ‘Yunmai 53’ and ‘Xikemai 8’,which were selected as low nitrogen and high efficiency cultivars. Six cultivars,such as ‘Mianmai 1419’ and ‘Xikemai 8’,which were selected as high nitrogen and high efficiency cultivars. Under low nitrogen treatment,nitrogen efficient wheat cultivars had higher TNA,SDW,SRA,RDW and LA. Under high nitrogen treatment,nitrogen efficient wheat cultivars had RDW,SDW,TNA and SPAD,LA and SRA. Under low nitrogen stress or high nitrogen treatment,nitrogen efficient wheat cultivars could form stronger root system and stronger aboveground parts than nitrogen inefficient wheat cultivars. 【Conclusion】 The above comprehensive evaluation methods are used to screen the index system of nitrogen efficient wheat cultivars scientifically and effectively. Under the condition of water culture,SDW,RDW,LA,SRA and TNA can be used as evaluation indexes of nitrogen efficient wheat cultivars at seedling stage. 4 low nitrogen high efficiency cultivars and 6 high nitrogen high efficiency cultivars were selected.
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Research on the Logic and Path of Promoting the Construction of Common Prosperity in the Western Regions
ZHANG Shemei
Journal of Sichuan Agricultural University    2022, 40 (2): 286-291.   DOI: 10.16036/j.issn.1000-2650.202203054
Abstract231)      PDF(pc) (1262KB)(99)       Save
【Objective】 Common prosperity is the essential requirement of socialism and the consistent fundamental value orientation of the communist party of China. As the region with the largest area, the most low-income population, the weak economic development momentum and just elimination of absolute impoverishment area, the western region is undoubtedly facing more severe challenges on the road to common prosperity. 【Method】 From the perspective of dialectical relationship between productivity and production relations. Combing and defining the connotation of common prosperity, based on the new situation and new demands faced by the western region to promote the construction of common prosperity, the development idea of how to make the cake bigger and the distribution mechanism of how to cut the cake, explained the promotion of common prosperity in the western region. The internal logic of construction. 【Conclusion】 Finally, from promoting the revitalization of industries in the western poverty-stricken areas, improving the efficiency of source utilization, building a mixed rural economy, increasing the development of the western region, reforming the income distribution adjustment mechanism, innovating the urban-rural linkage model, improving the social security service system, and strengthening the construction of ecological civilization. Put forward a path to promote common prosperity in the western region.
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Effects of Plant Density,Irrigation and Fertilization Postponement on Growth,Yield and Quality of Zhengmai 369
HOU Ying, DING Jinping, ZHU Xinyu
Journal of Sichuan Agricultural University    2022, 40 (3): 346-352.   DOI: 10.16036/j.issn.1000-2650.202202071
Abstract228)      PDF(pc) (1338KB)(56)       Save
【Objective】 In order to evaluate the yield and quality of Zhengmai 369 more objectively and accurately,the high-yield and green high-efficiency cultivation of wheat were explored in this paper. 【Method】 Zhengmai 369 was used as material to study the effects of different winter wheat density,irrigation and fertilization postponement on growth,yield and quality of winter wheat in this paper. Three factors were included:sowing density (D1:270 kg/hm2,D2:180 kg/hm2),irrigation time (W1:irrigation at erecting stage,W2:irrigation at shooting stage) and fertilization time (F1:fertilization at erecting stage,F2:fertilization at shooting stage). The principal component analysis and weighted membership function analysis were used to evaluate the effects of different treatments on the yield and quality of Zhengmai 369. 【Result】 The results showed that the number of basic seedling,total stem number,plant height and leaf area index of Zhengmai 369,in vegetative growth stage under high density treatment were,significantly higher than those under low density treatment,while the number of tillering and secondary roots showed the opposite tread. The yield of Zhengmai 369 was significantly higher under irrigation and fertilization treatment at shooting stage than that of other treatments at the same density under both high density and low density. The main reason is that the thousand-grain weight is higher. The six indexes of Zhengmai 369 flour quality under different treatments were comprehensively evaluated by Z-score method. The results showed that irrigation and fertilization postponement was beneficial to improve the flour quality. Principal component analysis and weighted membership function analysis showed that low sowing density,fertilization and irrigation postponement could increase yield,improve the flour quality of Zhengmai 369. 【Conclusion】 The results indicated that appropriate management measures can realize the coupling of high yield and high quality of Zhengmai 369.
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Study on Involvement of Cytoplasmic Glyceraldehyde-3-Phosphate Dehydrogenase GAPC2 is Involved in the Regulation of Tomato Fruit Ripening
YANG Min, LONG Yu, WANG Liangxin, LIU Xiaoyang, HOU Guoyan, JIANG Yuyan, LUO Ya
Journal of Sichuan Agricultural University    2022, 40 (2): 156-162.   DOI: 10.16036/j.issn.1000-2650.202108019
Abstract208)      PDF(pc) (5455KB)(112)       Save
【Objective】 This paper aims to study the effect of heterologous cytoplasmic glyceraldehyde-3-phosphate dehydrogenase 2 (GAPC2) gene on tomato fruit ripening. 【Method】 The strawberry (Fragaria × ananassa ‘Benihoppe’) FaGAPC2 overexpression vector was constructed,and transient overexpression technology was used to overexpress FaGAPC2 during the breaking period of cherry tomato (Solanum lycopersicum ‘luona’). 【Result】 The experimental results showed that the expression level of FaGAPC2 was 2.39-fold that of the control. Overexpression of FaGAPC2 can significantly inhibit the coloring of tomato peel and the accumulation of the total soluble solids content,while increase its hardness to 3.76N. Meanwhile,the contents of carotenoid and total chlorophyll were about 0.37-fold and 1.5-fold higher than that of the control,respectively. Further real-time fluorescence quantitative analysis results showed that overexpression of FaGAPC2 can significantly reduce the expression of 1-aminocyclopropane-1-carboxylate oxidase1 (ACO1,12.89-fold),and 1-aminocyclopropane-1-carboxylate oxidase3 (ACO3,3.87-fold),and ethylene-responsive element factors1 (ERF1, 3.51-fold) in the ethylene synthesis pathway;phytone synthase1 (PSY1,12.39-fold),and chromoplast-specific lycopene-β-cyclase (CYC-B,1.49-fold)in the carotenoid synthesis pathway;fruit ripening-associated genes:peptide methionine sulfoxide reductase4 (E4,4.48-fold),polygalacturonase (PG,41.35-fold) and the MADS transcription factor ripening inhibitor (RIN,6.67-fold). In note,the expression level of fruit ripening-related genes 1-aminocyclopropane-1-carboxylate oxidase -like (E8) and lipoxygenase (LOXB) were almost zero compared to the control. 【Conclusion】 It showed that FaGAPC2 is involved in the regulation of tomato fruit ripening as a negative regulator.
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Study on Comparison of Regeneration Capacity of Different Varieties of Common Bean
CHENG Yuan, MA Pengtao, WU Jing
Journal of Sichuan Agricultural University    2022, 40 (4): 465-471.   DOI: 10.16036/j.issn.1000-2650.202202080
Abstract205)      PDF(pc) (14915KB)(94)       Save
【Objective】 To select the appropriate regeneration genotypes of common beans,to build the construction of the common beans transformation system laid the foundation. 【Method】 Select 19 common beans, the embryonic axis of the cotyledons was used as explants, regenerative buds were induced on bud-inducing medium containing 2 mg/L 6-BA, 0.5 mg/L TDZ and 2 mg/L AgNO3. The germination rate, regeneration bud rate and the average number of regenerated buds were counted, and observed the status of the regeneration bud and the regeneration bud rate. Comprehensive screening of suitable regeneration materials. 【Result】 The regeneration bud rate of Xibaiyang Jiaodou was 94.4%, which is significantly different from other common beans. 【Conclusion】 It is preliminarily concluded that the Xibaiyang Jiaodou is more suitable to construct the genetic transformation system among 19 common beans.
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Research Progress of Lesion Mimic Mutants in Main Plants and Its Implication for Wheat Related Studies
MA Jian, LI Cong, TIAN Rong, TANG Huaping, $\boxed{\hbox{LAN Xiujin}}$, YAO Qifu
Journal of Sichuan Agricultural University    2022, 40 (5): 633-644.   DOI: 10.16036/j.issn.1000-2650.202204100
Abstract198)      PDF(pc) (5446KB)(128)       Save
【Objective】 To understand the research progress and regulation mode associated with the formation of lesion mimic in plants,and lay a foundation for genetic improvement of leaf traits and mining of disease-resistant genes,we reported on progress on lesion mimic genes in main plants. 【Method】 The origin,nomenclature,classification, genetic mechanism and disease resistance of lesion mimic mutants were summarized and analyzed by collecting and reviewing the relevant literature in this article. 【Result】 The formation of lesion mimic spots may affect the growth and development of plants. Furthermore, the formation of lesion mimic spots is similar with hypersensitive response leading to programmed cell death and enhancing the resistance to disease. Numerous studies have proved that lesion mimic genes play an important role in biological stress in main plants including Arabidopsis thaliana, rice, maize, and barley. 【Conclusion】 Many lesion mimic genes have been reported in model plants such as Arabidopsis thaliana and rice. The research of lesion mimic genes in other crops (including wheat) is relatively slow. However, research progress of lesion mimic genes in the main plants and the development of sequencing technology,molecular biology and transgenic technology will greatly accelerate the cloning and dissecting of regulatory mechanisms of lesion mimic genes in wheat. Lesion mimic genes have important theoretical values and application prospect in the future.
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Analysis of Cultivated Land Use Efficiency and Influencing Factors in the Yangtze River Delta
GUI Zheng, YU Ran, QIN Yan, LI Lanlan, SONG Zhongjun
Journal of Sichuan Agricultural University    2021, 39 (6): 843-849.   DOI: 10.16036/j.issn.1000-2650.2021.06.019
Abstract195)      PDF(pc) (2428KB)(87)       Save
【Objective】 In order to improve the cultivated land use efficiency in the Yangtze River Delta region, the paper studied the overall and regional cultivated land use efficiency and its influencing factors. 【Method】 Using data envelopment analysis (DEA), Tobit model analysis and mathematical analysis. 【Result】 ①The average utilization efficiency of cultivated land in the Yangtze river Delta region in the past 19 years was 0.81 and fluctuated upward, according to regional differences, 27 cities could be divided into high efficiency, higher efficiency, medium efficiency and low efficiency zones; ②Tobit model was used to analyze the effects of various influencing factors on cultivated land use efficiency in the Yangtze River Delta. Per capita GDP, cultivated land multiple cropping index, fertilizer application rate per unit cultivated land area, per capita cultivated land area and capita disposable income of rural residents had significant effects on cultivated land use efficiency;③The current high-efficiency agricultural finance and production policies should be continued in high efficiency zones, in higher-efficiency areas should effectively control the intensity of fertilizer use, medium-efficiency areas should correctly handle the relationship between regional economic development and the amount of arable land, low efficiency should attach importance to agricultural farming while developing the economy. 【Conclusion】 At present, the efficiency of cultivated land use in the Yangtze River Delta is at a medium level, which presents regional differences under the joint action of multiple factors. Therefore, it is an effective way to improve the utilization efficiency of cultivated land in the Yangtze River Delta by regional discussion, analyzing the effect degree of its influencing factors and exploring the internal mechanism of its characteristics.
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Ant Species Diversity of Eastern Daliangshan, Sichuan Province
HAN Xiu, XU Zhenghui, ZHANG Xinmin, LI Biao, ZHAI Jiang, LI Ting
Journal of Sichuan Agricultural University    2021, 39 (6): 742-754.   DOI: 10.16036/j.issn.1000-2650.2021.06.006
Abstract185)      PDF(pc) (5408KB)(144)       Save
【Objective】 To reveal the diversity law of ant in eastern Daliangshan, Sichuan Province. 【Method】 Ant species diversity in the region was investigated by using plot sampling method. Estimate S9.1.0 software was used to process data and to analyze the adequacy of sampling. Diversity analysis method was used to analyze community characters, including community composition, species diversity and similarity. Pearson correlation analysis was used to analyze the relationship between ant species diversity and vegetation feature. 【Result】 A total of 35 630 ant individuals were collected from 8 vertical zones in eastern Daliangshan, which belonging to 136 species, 43 genera and 6 subfamilies. Ant individual density was 2.0-399.8 heads/m2 (average 96.9 heads/m2), the bottom domain effect was significant at the north slope of each vertical zone, and the multi domain effect was obvious at the south slope; the dominant indexes varied greatly among different plots, and the overall multi domain effect was obvious; diversity indexes were greatly affected by the slope aspect, the north slope showed mid-domain effect phenomenon,and the south slope mostly expressed multi-domain effect. The similarity coefficient was 0.158 5-0.583 3 (average 0.259 8), which was in the medium dissimilar level. 【Conclusion】 Ant species diversity was affected by latitude, slop aspect and altitude. Altitude was the determinant element of the main indexes of ant communities. The number of species, individual densities and diversity indexes decreased with the increase of altitude, which were influenced by slop aspect, climate and human factors. Although ant species diversity from all the vertical zones have been disturbed in some way from human activities, the similarity coefficient is still low, indicating that different altitudinal zones have significant differences among ant communities, which has good ecological function and conservation value.
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Characteristics and Influencing Factors of Ammonia Volatilization in Garlic Field
CHEN Yanqiu, CHEN Jie, HU Zhitao, ZHANG Yifan, YAN Fengjun, REN Wanjun, ZHOU Wei
Journal of Sichuan Agricultural University    2022, 40 (1): 58-66.   DOI: 10.16036/j.issn.1000-2650.202109027
Abstract178)      PDF(pc) (9159KB)(62)       Save
【Objective】 The characteristics of ammonia volatilization in garlic field and the effects of environmental conditions and cultivation measures on ammonia volatilization were expounded to provide scientific basis for optimal nitrogen application of garlic cultivation. 【Method】 The venting method was used for the in-situ determination of ammonia volatilization rate in a long-term garlic planting field, and the field atmosphere and soil conditions were determined synchronously. Besides, a three-factor experiment, with different N-fertilizer application amount, straw mulching treatments, and different N-fertilizer type, was conducted to study the influencing factors of ammonia volatilization in garlic field. 【Result】 ①Ammonia volatilization mainly occurred in the first week after fertilization, and the ammonia volatilization rate increased first and then decreased. The total ammonia volatilization amount accounted for 9.2% of the total N-fertilizer application amount, and the ammonia volatilization rate of the base fertilizer was the highest. ②Ammonia volatilization rate was significantly positively correlated with air temperature, light intensity, soil temperature and ammonium ion concentration. ③The rate and amount of ammonia volatilization increased significantly with the increase of N-fertilizer application amount. Compared with the three different types of N-fertilizer, ammonia volatilization of ammonium bicarbonate and urea was significantly higher than that of compound fertilizer, and the difference also increased with the increasing of N-fertilizer application amount. ④Straw mulching after fertilization could reduce ammonia volatilization, and the reducing effect was more obvious under low N-fertilizer application amount. 【Conclusion】 The results indicated that the large amount of N-fertilizer application was the main reason for the high ammonia volatilization in garlic field. To reduce the N-fertilizer application amount, straw mulching after fertilization, and replace ammonium bicarbonate and urea with N-fertilizer with lower ammonia volatilization risk could reduce the ammonia volatilization effectively in garlic field.
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Analysis of Activity Components Involved in Decreasing Blood Sugar,Blood Lipid and Antioxidant Capacity in Rice Varieties
GUO Qiaoling, YANG Ying, YANG Xiaoli, LIN Dan, QIANG Runrun, JIA Feng, ZHANG Hongyu, XU Peizhou, LIAO Yongxiang, WANG Jing, ZHOU Hao, LIU Yutong, WU Xianjun, CHEN Xiaoqiong
Journal of Sichuan Agricultural University    2022, 40 (1): 42-49.   DOI: 10.16036/j.issn.1000-2650.202109005
Abstract176)      PDF(pc) (3719KB)(94)       Save
【Objective】 The study compared physiological function in different pigmented rice varieties and analyzed activity components,in order to provide theoretical basis for breeding of rice. 【Method】 The study measured the total protein content using the Kjeldahl method,the total starch content by colorimetric method,the content of resistant starch by the Megazyme kit. The total polyphenol was checked using the Folin method. The flavonoid content was measured by the NaNO2-Al(NO)3 colorimetric method. The study also detected the antioxidant activity by ORAC method in vitro,and blood sugar and lipid by Kunming mice experiment. 【Result】 Three different pigmented rice varieties were used to determine the total protein content,starch content,total phenolic and flavonoid content. Three rice materials were as follows:white rice (Shuhui 527),red rice (Hongxiangnuo) and black rice (Heixiangnuo). The results showed that the total protein, starch and resistant starch content hardly existed in significant differences among three different pigmented rice varieties. The results of total polyphenols content showed that red rice Hongxiangnuo (12.6 mg/g) was the highest,following by black rice Heixiangnuo (8.0 mg/g),the lowest was white rice Shuhui527 (5.6 mg/g). Besides,the tendency of total flavonoid content in three rice varieties was the same as that of polyphenol content. The results of antioxidant capacity showed that red rice Hongxiangnuo (363 μmol Trolox/g) for antioxidant activity presented the highest,following by black rice Heixiangnuo (219 μmol Trolox/g),the lowest was white rice Shuhui 527 (107 μmol Trolox/g) by vitro. Red rice Hongxiangnuo was stronger for hypoglycemic by vivo. On the contrary,blood lipids level reduced just presented in black rice Heixiangnuo group. The study further found that red rice Hongxiangnuo group had the highest SOD value as the same as antioxidant activity in vitro, and attained 556.65 U/mg. 【Conclusion】 Red rice (Hongxiangnuo) especially possesses the strongest physiological activity,following by black rice (Heixiangnuo),the lowest in white rice (Shuihui527),because red rice (Hongxiangnuo) and black rice (Heixiangnuo) contained higher phenolics and flavonoid content.
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Preparation and Quality Evaluation of Warm Sensitive Gel for the Compound ‘Zicao’
XU Ke, WU Yunjie, LI Jinyang, ZHAO Junxi, ZHANG Zehan, LIANG Xiaoxia
Journal of Sichuan Agricultural University    2021, 39 (6): 791-798.   DOI: 10.16036/j.issn.1000-2650.2021.06.012
Abstract170)      PDF(pc) (2509KB)(94)       Save
【Objective】 To prepare and evaluate the warm sensitive gel of compound ‘Zicao’. 【Method】 The warm sensitive gel of compound ‘Zicao’ was prepared with Poloxamer by cold solution. Taking the gelation temperature as an index,the formulation of the gel was optimized. The quality of the prepared gel was evaluated according to physicochemical property, and the microbial limit. 【Result】 The best prescription of the warm sensitive gel was prepared by 16% P407, 2% P188, 4% glycerol and 8% compound ‘Zicao’ extraction. The average gelation temperature of 32.8 ℃ was obtained. 【Conclusion】 The compound ‘Zicao’ warm sensitive gel is stable in quality and suitable in gelation temperature,providing a better new choice for the healing of various wounds.
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Yield Traits Analysis of New Wheat Lines (Varieties) in Upper Reaches of Yangtze River Basin of National Trial During 2009—2020
LI Shizhao, TU Yang, ZHU Huazhong, LYU Jijuan, ZHENG Jianmin, WAN Hongshen, LUO Jiangtao, YANG Manyu, WU Ling
Journal of Sichuan Agricultural University    2022, 40 (1): 19-27.   DOI: 10.16036/j.issn.1000-2650.202110011
Abstract168)      PDF(pc) (10862KB)(121)       Save
【Objective】 The evolution law of yield and yield-related traits of new wheat lines (varieties) in upper reaches of Yangtze River basin in recent years was analyzed,which can provide some theoretical basis for breeding and production of wheat cultivars in this region. 【Method】 Based on the yield,yield components and yield-related agronomic traits of national trial in the upper reaches of Yangtze River basin from 2009 to 2020,the evolution law and correlation were analyzed. 【Result】 The coefficient of variation of the tested lines in the upper reaches of Yangtze River basin in 12 years was as follows:whole growth duration < thousand kernel weight < plant height < number of effective ear < yield < number of grains per spike. The annual average yield of the tested lines increased slowly as the years progressed,ranging from 4 944.6 kg/hm2 to 6 087.8 kg/hm2,with a total average of 5 464.3 kg/hm2. Among the yield components,the number of grains per spike increased most obviously with the advance of the year,followed by the number of effective ear,while the thousand kernel weight decreased slowly. For the main yield-related agronomic traits,the whole growth duration of tested lines was more dramatically affected by the environment,while the increase of yield was significantly limited by plant height. Correlation analysis showed that the yield was extremely negatively correlated with plant height. 【Conclusion】 There was no significant difference in the yield level between the tested lines and the check in 12 years. The number of grains per spike was the key factor to improve the yield in the upper reaches of the Yangtze River basin. Choosing varieties with moderate plant height and lodging resistance is more propitious to stable production.
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Study on the Production Increasing Potential and Guarantee Capability of Grain in China in the Next 30 Years
CHEN Yuanyuan, SUN Yi
Journal of Sichuan Agricultural University    2022, 40 (3): 312-318.   DOI: 10.16036/j.issn.1000-2650.202202034
Abstract164)      PDF(pc) (1406KB)(98)       Save
【Objective】 This paper aims to evaluate production increasing potential and total demand of grain in China in the next 30 years, scientifically judge China’s future food security capacity and put forward policy implications. 【Method】 Based on the GAEZ model, this study systematically evaluated the production increasing potential of grain and four main grain crops in China and various provinces were, then used the VAR model and itemized estimation method to predict the grain demand in the next 30 years. At last, the future grain guarantee capability was analyzed. 【Result】 The production increasing potential of rice, wheat, maize and soybean was 108 million ton (51.30%), 48 million ton (36.05%), 233 million ton (89.16%) and 25 million ton (153.75%) respectively in 2020—2050, and the overall production increasing potential of grain was 442 million ton (66.56%), of which the grain production increasing potential in main grain producing areas accounted for 74%. The rice and wheat demand was expected to peak at 216 and 143 million ton in 2035, maize and soybean demand was expected to peak at 338 and 139 million ton in 2045, respectively, and total grain demand was expected to peak at 902 million ton in 2040. 【Conclusion】 In the next 30 years, China is fully capable of ensuring “absolute security of rations and basic self-sufficient of cereals”, as well as raising the self-sufficiency ratio of soybeans to 30%. The key is to effectively tap the potential of grain production from the aspects of the development of reserve cultivated land resources, the construction of high-standard basic farmland, the research and promotion of agricultural science and technology, and the guidance and optimization of rural land transfer.
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Development and Application of Molecular Marker for the Key Gene EjPSY2A of Flesh Color Regulation in Loquat
SONG Haiyan, SUN Shuxia, CHEN Dong, LI Jing, TU Meiyan, XU Zihong, LOU Hongxia, GONG Ronggao, JIANG Guoliang
Journal of Sichuan Agricultural University    2022, 40 (2): 172-178.   DOI: 10.16036/j.issn.1000-2650.202201050
Abstract158)      PDF(pc) (13816KB)(70)       Save
【Objective】 This paper aims to develop a reliable molecular marker for loquat flesh color identification at juvenile period so as to accelerate the cultivar improvement process. 【Method】 In this study,we designed two InDel molecular markers were designed based on the sequence difference of phytoene synthase gene EjPSY2A between yellow- and white-fleshed loquat varieties. A primer PSY2A-2 with clear genotyping results was selected after preliminary screening,and then its identification accuracy was detected with 85 loquat germplasm resources and hybrids worldwide,also the sequence of amplification product with the primer PSY2A-2 was further confirmed using TA cloning method. 【Result】 The results showed that all 42 white-fleshed loquat samples possessed homozygous EjPSY2Ad genotype,all 43 yellow-fleshed loquat samples possessed homozygous EjPSY2A genotype or heterozygous EjPSY2A and EjPSY2Ad genotype. It was confirmed that the amplification product of ‘Dawuxing’ was a homozygous EjPSY2A allele with TA cloning method. 【Conclusion】 The newly-developed InDel molecular marker PSY2A-2 can accurately distinguish the yellow- and white-fleshed loquat germplasm resources,which has a significant meaning for early selective breeding in loquat hybrids and can help to accelerate the process of loquat breeding.
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Effects of Bio-Fertilizer Partially Substituting Chemical Fertilizer on Soil Fertility and Enzyme Activity in Maize Field
ZHU Lixia, CAO Mengmeng, SANG Chengchen, CHEN Rubing, XU Siwei, LI Lili, LIU Tianxue
Journal of Sichuan Agricultural University    2022, 40 (1): 67-72.   DOI: 10.16036/j.issn.1000-2650.202107017
Abstract156)      PDF(pc) (1373KB)(103)       Save
【Objective】 In order to investigate the effect of bio-fertilizer partially substituting chemical fertilizer on soil nutrient condition and enzyme activity, and improve the problems of soil fertilization reduction caused by excessive fertilizer application. 【Method】 A maize hybrid Xundan 20 was used in a field experiment with five treatments (CF, 100% chemical fertilizer N; BF5, 95% chemical fertilizer N + 5% bio-fertilizer N; BF10, 90% chemical fertilizer N + 10% bio-fertilizer N; BF20, 80% chemical fertilizer N+20% bio-fertilizer N; BF30, 70% chemical fertilizer N + 30% bio-fertilizer N). The soil organic matter, total nitrogen, activities of urease, sucrose, alkaline phosphstase and catalase and maize yield were determined in different treatments. 【Result】 Bio-fertilizer combined with chemical fertilizer significantly increased soil organic matter and total nitrogen contents, of which BF30 shared the highest increases of 25.43% and 31.87%, respectively. Increases of soil available nutrient contents first increased and then decreased with the increasing of bio-fertilizer application rates. The highest available nitrogen, phosphorus and potassium were recorded in BF20 treatment. The increases of urase and catalase activities were 15.13%-24.72% and 76.83%-89.02% compared with CF treatment, respectively. Bio-fertilizer application markedly increased activities of soil sucrase and alkaline phospatase by 4.61%-28.93% and 6.96%-23.93%, respectively, of which the highest increases were observed in BF20 treatments. The maize yield was significantly increased by bio-fertilizer, and the highest increase was found in BF20, which reached 23.79%. 【Conclusion】 The soil fertility and maize grain yield were increased by bio-fertilizer, and it has a better effect that replacing 20% chemical fertilizer N with bio-fertilizer N in this region.
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Influence of Capital Endowment on Farmers’ Adoption Scale of Rice and Shrimp Co-culture Technology:Mediating Effects Based on Technology Cognition
WANG Xicong, QI Zhenhong, YANG Caiyan, LIU Zhe
Journal of Sichuan Agricultural University    2022, 40 (1): 130-136.   DOI: 10.16036/j.issn.1000-2650.202010007
Abstract153)      PDF(pc) (1447KB)(65)       Save
【Objective】 To reveal the mechanism of the influence of capital endowment and technology perception on the scale of rice-crayfish coculture technology adoption among farmers. 【Method】 OLS model and Bootstrap test methods were used for empirical analysis. 【Result】 ① Natural capital, economic capital, social capital and human capital all had a significant positive impact on the scale of farmers’ adoption of rice-crayfish coculture technology, and the impact effects were ranked from largest to smallest as follows: Economic capital>Natural capital>Human capital>Social capital;② There is an inverted U-shaped relationship between capital endowment and the adoption scale of rice-crayfish coculture technology;③ Perceived ease of use plays an intermediary role in the impact of peasant households’ capital endowment on the adoption scale, but perceived usefulness has no intermediary role. 【Conclusion】 In the promotion of rice-crayfish coculture technology, attention should be paid to the accumulation of farmers’ economic capital, and multi-channels should be used to enhance farmers' perception of the ease of use of rice-crayfish coculture technology, but appropriate scale management should be considered.
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Study on the Change of Stress Resistance Marker and Germination Rate of Zhenghong 431 Seeds with Different Maturity
HE Zhen, QUBI Wuhe, CAI Lin, KE Yongpei, YU Xuejie, DING Tianli, SHI Haichun
Journal of Sichuan Agricultural University    2022, 40 (1): 36-41.   DOI: 10.16036/j.issn.1000-2650.202104014
Abstract152)      PDF(pc) (1291KB)(79)       Save
【Objective】 The appropriate harvest period of the maize hybrids Zhenghong 431 in Chengdu and Xichang was determined,which will provide a technical support for the production of high-quality seeds. 【Method】 The appropriate harvest period was judged by comparing the activities of stress resistance marker of malondialdehyde (MDA),peroxidase (POD),superoxide dismutase (SOD),and the content of starch,protein,fat,as well as the seed germination rate. 【Result】 In Chengdu,the MDA and POD content of Zhenghong 431 tended to be stable at 35th and 40th days after pollination,respectively,while the SOD contents reached the peak at the 45th days after pollination. The starch,protein and fat content of Zhenghong 431 reached stable at 35th,40th and 35th days after pollination,respectively. The germination rate of Zhenghong 431 was over 90% at 30th days after pollination and reached 100% at 45th days after pollination. In Xichang,the MDA content of Zhenghong 431 tended to be stable at 40 days after pollination. The POD and SOD contents of Zhenghong 431 reached the peak at the 45-60th days,55-60th days after pollination,respectively. The starch,protein and fat content of Zhenghong 431 were stable at 40th,45th and 45th days after pollination,respectively. The germination rate of Zhenghong 431 reached 94% in 25th days and 100% in 60 days after pollination. 【Conclusion】 Combining the stress resistance marker,storage substance and germination rate,we conclude the suitable harvest periods for Zhenghong 431 seeds in Chengdu and Xichang are 45 days and 60 days after pollination,respectively.
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Effects of the Different Tillage Methods and Sowing Rates on Yield and Morphogenesis of Wheat in the Rice-Stubble Wheat System
SUN Jiawei, LI Hao, YAN Hong, HE Yutin, WANG Ke, ZHANG Cheng, LI Jitang, YANG Xun, WU Jikai
Journal of Sichuan Agricultural University    2022, 40 (1): 28-35.   DOI: 10.16036/j.issn.1000-2650.202106015
Abstract152)      PDF(pc) (1368KB)(170)       Save
【Objective】 To explore the effects of the tillage methods and sowing rates on the growth of wheat in the rice-stubble system. 【Method】 Three different sowing rates (D1-150 kg/hm2,D2-210 kg/hm2 and D3-270 kg/hm2) with three tillage methods including compound tillage (T1),shallow rotating (T2) and no-tillage straw mulching (T3) to invest the yield and morphogenesis of wheat in the rice-stubble wheat system. 【Result】 The results showed the treatment of T1 with D2 could have the highest yield at 7.92 kg/hm2. For the different tillage methods,the yield,the effective panicle number,the panicle rate,the dry matter weight of population at the different growth stages,the outer diameter of the internode of culm at the heading stage and plant height of wheat showed the tendency that T1 > T2 > T3. The number of grains per panicle of wheat in T2 was 3.61 panicle-1 and 4.32 panicle-1 higher than that in T1 and T3,respectively. The treatment of T2 could coordinate the relationship between the effective panicle and the number of grains per panicle,increase the dry matter accumulation ratio after booting stage,and have the longest flag leaf at the heading stage and the longest panicle at the maturity stage. Meanwhile,increasing sowing rate could increase the grain yield,the effective panicle number and the dry matter of population of wheat at the different growth stages,but decrease the grain number per panicle and the dry matter accumulation ratio after the booting stage in the different tillage methods. For the high sowing rates (270 kg/hm2),T1 could significantly increase the effective panicle number,plant height,and panicle length,but decrease the diameter of the internode of the culm. Furthermore,increasing sowing rate could increase the 1 000-grain weight,decrease the length of flag leaf at the heading stage,panicle length and plant height. 【Conclusion】 Wheat in the rice-stubble could have highest the effective panicle,number of seeds,dry matter accumulation in maturity population and grain yield.
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Allelopathic Effect of Eucalyptus grandis × E. urophylla Essential Oil on Lolium perenne and Triticum aestivum Seeds
KONG Qingbo, YANG Shuai, WANG Xiaoju, LI Jiajia, LUO Siyuan, DING Chunbang, ZHOU Lijun
Journal of Sichuan Agricultural University    2021, 39 (6): 713-720.   DOI: 10.16036/j.issn.1000-2650.2021.06.002
Abstract152)      PDF(pc) (3119KB)(88)       Save
【Objective】 To explore the potential allelopathic effects of Eucalyptus grandis × E. urophylla leaves essential oil (EEO) on Lolium perenne and Triticum aestivum seeds. 【Method】 By steam distillation extract EEO, using gas chromatography-mass spectrometry (GC-MS) combination of the essential oil composition analysis and measuring its allelopathy of perennial Lolium perenne and Triticum aestivum seeds related indicators. 【Result】 The extraction rate of essential oil was 0.58%, the main ingredients were terpinyl acetate, eucalyptus oil, alcohol, pinene and alpha terpineol. Among them, terpinyl acetate (24.604%) content was higher than that of eucalyptus oil alcohol (19.843%) and others. The germination rate, germination force, germination index, shoot length, root length, chlorophyll, and malondialdehyde contents of Lolium perenne and Triticum aestivum seeds treated with EEO of different concentrations were significantly lower than those of the control group, and showed dose-effect. In particular, the essential oil concentration of Lolium perenne at 2.000 mg/mL was significantly different from that of the control group. In perennial Lolium perenne, the response of Triticum aestivum allelopathic index, besides malondialdehyde, the rest of the seven biometric indicator feeling response index were almost all negative, and with the essential oil concentration of the absolute value of the response of the allelopathic index increased gradually. 【Conclusion】 The allelopathic effect of EEO on the tested plants was inhibitory, and the intensity of the inhibitory effect was related to the concentration of essential oil and the type of seeds. This study may provides reference for the further development of environmental herbicide and rational crop cultivation by using EEO.
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Differences in Soluble Sugar Accumulation Pattern in Ziziphus jujuba Mill. and Ziziphus acidojujuba Cheng et Liu Fruits
DENG Qian, WANG Yang, DENG Qunxian, ZHANG Huifen, XIA Hui, LIN Lijin, LYU Xiaoping, LIAO Wenfei
Journal of Sichuan Agricultural University    2021, 39 (6): 734-741.   DOI: 10.16036/j.issn.1000-2650.2021.06.005
Abstract152)      PDF(pc) (5788KB)(84)       Save
【Objective】 Here, the differences in soluble sugar accumulations pattern between Ziziphus jujuba Mill. cv. ‘Luojiangtiaoyuanzao’ and Ziziphus acidojujuba Cheng et Liu cv. ‘Yuanxingxiaosuanzao’ fruits were studied. 【Method】 The fruits of ‘Luojiangtiaoyuanzao’ and ‘Yuanxingxiaosuanzao’ were used as test materials. The contents of soluble sugar components and the expression levels of metabolism-related genes at different developmental stages were analyzed to determine the different pattern of soluble sugar accumulation in jujube fruits. 【Result】 Both ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ contain sucrose, fructose and glucose. The fruits of ‘Yuanxingxiaosuanzao’ accumulated sucrose and hexose, whereas the fruits of ‘Luojiangtiaoyuanzao’ accumulated sucrose. A comparison showed that changes in the sucrose and hexose contents during late fruit development led to different soluble sugar accumulation pattern between the two cultivars. In the sucrose synthesis of ‘Yuanxingxiaosuanzao’, the sucrose metabolism-related genes Zj SS1, Zj SS2, Zj SS3, Zj SPS1 and Zj SPS2 played key roles, and sucrose metabolism-related genes Zj SS2, Zj SS3, Zj SPS1 and Zj SPS2 played key roles in ‘Luojiangtiaoyuanzao’. Zjv INV1, Zjc INV3 and Zjn INV1 played a key role in the synthesis of fructose and glucose in ‘Yuanxingxiaosuanzao’. 【Conclusion】 Thus, the differential expression of Zjv INV1, Zjc INV3 and Zjn INV1 is responsible for the different fructose and glucose accumulation pattern between ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ jujubes. The difference of sucrose accumulation pattern between ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ jujubes needs further study.
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Effects of Combined Application of Aspartic Acid Diethoxvsuccinate and Tartaric Acid on Growth and Cadmium Enrichment Characteristics of Amaranthus hybridus L.
HE Mingqian, LU Xin, TANG Yan, CHEN Zhiyin, XIA Juanwen, XU Xiaoxun, LI Qilan
Journal of Sichuan Agricultural University    2021, 39 (6): 827-834.   DOI: 10.16036/j.issn.1000-2650.2021.06.017
Abstract149)      PDF(pc) (5344KB)(79)       Save
【Objective】 The aim of the present study was to focus on the possibility of improving the phytoextraction of Cd by Amaranthus hybridus L. with the application of aspartic acid diethoxvsuccinate (AES) and tartaric acid (TA). 【Method】 Pot experiments were conducted to investigate the effects of AES and TA on the growth, antioxidant enzyme activity and Cd accumulation in A. hybridus. 【Result】 The application of AES had no significant effect on the biomass of A. hybridus, while TA alone addition, AES and TA combined application could significantly improve the biomass of plant. SOD activity in leaves increased significantly with the addition of TA. No such effect was observed for AES. Application of AES and TA alone or in combination enhanced the proportion of weak acid extractable Cd in soils, and the maximum proportion of weak acid extractable Cd when 0.067 mmol/kg TA and 5 mmol/kg AES were combined was 1.53 times than that of the control. The addition of AES and TA improved the concentration and accumulation of Cd in different organs of A. hybridus, and the highest shoot Cd accumulation increased by 147.37% under 0.067 mmol/kg TA treatment compared with the control. AES and TA combined application significantly increased the shoot bioconcentration factor, and the Cd purification rate were 1.24~2.08 times compared with the control. 【Conclusion】 Thus, the data demonstrate that the application of AES and TA improve the phytoextraction in A. hybridus.
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Analysis of Histological Characteristics of Muscle Fibers in Different Parts of Black Tibetan Sheep
ZHOU Li, WU Zhanyue, ZHANG Fengshuo, GUI Linsheng, HOU Shengzhen
Journal of Sichuan Agricultural University    2021, 39 (6): 778-783.   DOI: 10.16036/j.issn.1000-2650.2021.06.010
Abstract148)      PDF(pc) (979KB)(65)       Save
【Objective】 This study analyzed fiber characteristics in different parts of muscle to provide reference for full exploiting meat potential of Qinghai black Tibetan sheep(♂). 【Method】 Via ATPase histochemical staining and real-time quantitative polymerase chain reaction (qPCR), muscle fiber histological characteristics and MyHCs gene expression level from longissimus Dorsi (LD), biceps femoris (BF) and triceps femoris (TF) were detected in black Tibetan sheep. 【Result】 ①The diameter of type Ⅱ A and type Ⅱ B muscle fibers in LD and TB were significantly lower than that in BF (P<0.05). The cross-sectional area of type Ⅰ, Ⅱ A and ⅡB in BF were significantly higher than that of LD (P<0.05). The fiber density of type Ⅰ, type ⅡA and type ⅡB muscle was LD>TB>BF (P>0.05). ②The proportion of type ⅡA muscle fibers in longissimus dorsi muscle was higher than that in brachial triceps muscle and biceps femoris muscle, and the difference between longissimus dorsi muscle and biceps femoris muscle was significant (P<0.05). The proportion of type Ⅰ muscle fiber area in longissimus dorsi muscle was higher than that in brachial triceps (P>0.05) and biceps femoris (P<0.05). ③The gene expression level of MyHCⅡA in LD was significantly higher than that in BF (P<0.05), but had no significant difference compared with TB (P>0.05). The gene expression level of MyHCⅠ, MyHCⅡB and MyHCⅡX at different parts of LD had no significant difference (P>0.05). 【Conclusion】 This study revealed the distributional differences of fibromuscular characteristics in different parts, providing a theoretical reference for the grading processing of Black Tibetan sheep.
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Effects of Combined Application of Organic Fertilizer and NPK on Growth and Economic Characters of Camellia oleifera
JIANG Jiayan, YANG Huiqin, OUYNAG Wenying, AN Maoshan, YANG Xiaoju, LONG Xueyan, HU Yuling
Journal of Sichuan Agricultural University    2022, 40 (1): 73-82.   DOI: 10.16036/j.issn.1000-2650.202107026
Abstract148)      PDF(pc) (1351KB)(86)       Save
【Objective】 To explore the effects of combined application of organic fertilizer and NPK on the growth and economic characteristics of Camellia oleifera, and to provide scientific fertilization basis for the high yield and stable yield of Camellia oleifera. 【Method】 Taking the Camellia oleifera Xianglin 210# as the research object, the L27 (91+38) multi-factor mixed level orthogonal test was used to study the effects of fertilization time, fertilization methods, organic fertilizers, nitrogen, phosphorus and potassium fertilizers on the growth and fruit-related indicators of Camellia oleifera. 【Result】 Fertilization time has a significant impact on the growth of plant height and ground diameter of Camellia oleifera, the number of results and the weight of single fruit in the second year (P<0.05). The influence of tea seed moisture content is extremely significant (P<0.01). Fertilization methods have significant effects on the north-south canopy width, ground diameter growth, and seed oil content (P<0.05); N, P, and K have a significant impact on the growth of the north-south canopy width of Camellia oleiferaP<0.05); organic fertilizer has a significant impact on the growth of ground diameter (P<0.05). Treatment 15 increased the plant height more obviously, treatment 25 increased more ground diameter, treatment 6, treatment 18 and treatment 22 increased the crown width of the east and west, treatment 16 and treatment 15 had more obvious increase in the north and south crown width of Camellia oleifera; The yield and fruit quantity of two years of treatment 17 were higher, the fresh seed rate of treatments 20 and 26 was higher in the second year of fertilization, the seed rate of treatments 14, 20 and 24 was higher, and the kernel oil content of treatments 26, 10 and 5 was higher. 【Conclusion】 To sum up and consider the impact of the amount of fertilization and the increase in fertilizer on the cost of fertilization, the best fertilization technique is in June, applying organic fertilizer 750 g+ urea 100 g+ superphosphate 50 g+ potassium chloride 200 g in June; Or in October acupoint application of organic fertilizer 750 g+ urea 50 g+ superphosphate 50 g+ potassium chloride 100 g.
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Screening and Application of SSR Molecular Markers Associated with Walnut Blight Resistance
RUAN Ruoyu, WANG Ming, ZHU Tianhui, LI Shujiang, HAN Shan
Journal of Sichuan Agricultural University    2022, 40 (2): 206-213.   DOI: 10.16036/j.issn.1000-2650.202201047
Abstract145)      PDF(pc) (11051KB)(48)       Save
【Objective】 By developing and screening SSR molecular markers related to walnut bacterial blight’s resistance, this study aims to achieve an efficient and accurate resistance identification of different walnut bacterial blights. 【Method】 Based on the results of walnut transcriptome sequencing (RNA-Seq), SSR markers that may be related to resistance were developed, and SSR primers related to walnut bacterial blight’s resistance were verified in different anti-inductive materials. 【Result】 Using the genomic DNAs of 12 disease-resistant and infected lines as the template, a SSR marker related to walnut bacterial blight’s resistance was obtained, and this marker could be amplified in the disease-resistant material a band with the length of 320 bp (named RJR18-M320). Using this primer to carry out the resistance identification of 25 existing walnut germplasm resources, this study obtained 16 resistant materials and 9 susceptible materials. 【Conclusion】 A pair of molecular markers that can be used for auxiliary selection of walnut resistance to bacterial blight were screened out, which effectively accelerated the breeding process of walnuts resistant to bacterial blight.
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Optimization of the Extraction Process of Total Flavonoids from the Shoots of Pleioblastus amarus and Evaluation of Its Anti-Inflammatory and Antioxidant Activities in Vitro
YAN Junling, FAN Yang, QIN Chuan, OU Xue, ZHOU Rui, XIE Wei, CHEN Shujuan, AO Xiaolin
Journal of Sichuan Agricultural University    2022, 40 (2): 276-285.   DOI: 10.16036/j.issn.1000-2650.202009059
Abstract144)      PDF(pc) (14299KB)(60)       Save
【Objective】 The purpose of this experiment was to study the optimum total flavonoids extraction process and the antioxidant and anti-inflammatory activity in vitro. 【Method】 Using the shoots of Pleioblastus amarus as raw material, this study used ultrasound-assisted extraction method was used, single factor test and Box-Behnken design to select optimum extraction conditions and evaluate its antioxidant activity and anti-inflammatory activity in vitro. 【Result】 The optimum extraction conditions of total flavonoids were as follows:solid-liquid ratio 1∶30 g/mL,the ethanol concentration is 87%,microwave power 200 W,pH value is 6,extraction temperature 51℃,extraction time 23 minutes. Under these conditions,the extracted yield of the total flavonoids was up to 10.61%;When the total flavonoids concentration was 0.5 mg/mL,the FRAP value reached 3.04 mmol/L,and the scavenging rates of DPPH radical,hydroxyl radical and ABTS radical were 88.73%,60.22% and 72.38%,the IC50 values were 0.07 mg/mL,0.39 mg/mL and 0.23 mg/mL. Respectively, when the total flavonoid concentration was 1.20 mg/mL,the inhibition rate of NO production could reach 93.94%. 【Conclusion】 The extraction process of the total flavonoids of the shoots of pleioblastus amarus was feasible, and the total flavonoids extract had certain antioxidant and anti-inflammatory activity.
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Effects of Dietary Forage to Concentrate Ratio on Gene Expression Level of Nutrient Transporter in Small Intestine of Qinghai Black Tibetan Sheep
ZHOU Li, ZHANG Fengshuo, ZHANG Chunmei, YANG Baochun, GUI Linsheng, WANG Zhiyou
Journal of Sichuan Agricultural University    2022, 40 (1): 105-110.   DOI: 10.16036/j.issn.1000-2650.202109008
Abstract142)      PDF(pc) (4614KB)(82)       Save
【Objective】 This experiment was conducted to investigate the effects of different concentrate to forage ratio diets on mRNA levels of Peptide transporter 1 PEPT1, Sodium-dependent glucose transporters 1 SGLT1, Glucose transporter 1GLUT1) and Januskinase 2JAK2) in intestinal tissues of black Tibetan sheep. 【Method】 Sixty 2-month-old healthy black Tibetan male lambs with similar body weight (10.45±0.96)kg were randomly divided into 3 treatment groups with 20 lambs per treatment. Lambs were fed a total mixed diet with 70% concentrate+30% roughage (HC group), 50% concentrate+50% roughage (MC group) and 30% concentrate+50% roughage (LC group), respectively. After feeding for 120 days, the mucosal tissues of duodenum, jejunum and ileum of black Tibetan sheep were collected to determine the relative expression abundance of PEPT1, SGLT1, GLUT1 and JAK2 gene. 【Result】 The results showed as follows: ① In duodenum, the mRNA levels of PEPT1 in group HC was higher than that in group LC (P<0.05), and the mRNA expression of both SGLT1 and GLUT1 in group HC was significantly higher than that in the other two groups (P<0.01). ② In jejunum, the mRNA levels of PEPT1 in HC groups were significantly greater than those in MC and LC group (P<0.01), and the mRNA expression of JAK2 and SGLT1 in group MC was significantly lower than that in group HC (P<0.05). ③ In ileum, the mRNA levels of PEPT1 gene in group HC was significantly higher than that in the other two groups (P<0.01). 【Conclusion】 Dietary composition could significantly affect the expression of nutrient transporter gene in black Tibetan sheep, which may directly or indirectly affect the metabolic function of small intestine, and the effect is better when the concentrate to forage ratio is 70∶30.
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Effect of Foliar Application of Potassium Dihydrogen Phosphate and Ethephon on the Synchronous Maturation and Quality of Upper Leaves of Flue-Cures Tobacco
ZENG Shuhua, WU Runsheng, GUO Shiping, XIA Chun, LIU Lei, LEI Xiao, CHEN Xiangjun, ZHANG Yonghui, LIU Yajie
Journal of Sichuan Agricultural University    2022, 40 (4): 565-573.   DOI: 10.16036/j.issn.1000-2650.202111048
Abstract141)      PDF(pc) (6434KB)(39)       Save
【Objective】 This study aims to study the effects of the concentration and spraying time of potassium dihydrogen phosphate and ethephon on the synchronous maturity and quality of upper tobacco leaves. 【Method】 The Box-Behnken method of the response surface optimization design was used to carry out the experimental design, intending to investigate three factors and three levels of spraying concentration of potassium dihydrogen phosphate (0、3.4、6.8 g/L), ethephon (0、0.05、0.1 g/L) and spraying time (20、25、30 days after topping). A total of 16 spray treatments and the indexes related to the mature state of tobacco leaves from top to bottom 1-3 pieces and top 4-6 pieces were measured. The appearance quality and chemical composition of flue-cured tobacco were also evaluated. 【Result】 Spraying potassium dihydrogen phosphate and ethephon could promote the synchronous maturity of the upper tobacco leaves, and the effect of treatment T2 (20 days after topping, combined with spraying 3.4 g/L potassium dihydrogen phosphate and 0.1 g/L ethephon) was the best. The appearance quality score increased by 0.45-7.7 points compared with control, the Comprehensive evaluation score of flue-cured tobacco was 0.82-6.33 points higher than control, and the score of T2 was the highest (79.60). It can be predicted that when the spraying time is 20 days after topping, the concentration of potassium dihydrogen phosphate is 3.808 g/L and the concentration of ethephon is 0.081 g/L, the synchronous maturity of upper tobacco leaves is the best and the quality of flue-cured tobacco leaves is the best. 【Conclusion】 Synchronous maturity of upper tobacco leaves was advanced and tobacco quality was enhanced by spraying appropriate concentration of potassium dihydrogen phosphate and ethephon at 20 days after topping.
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Differences in the Organic Acid Metabolism of Ziziphus jujuba Mill. and Ziziphus acidojujuba Cheng et Liu Fruits
DENG Qian, WANG Yang, DENG Qunxian, ZHANG Huifen, XIA Hui, LIANG Dong, WANG Quan
Journal of Sichuan Agricultural University    2022, 40 (2): 198-205.   DOI: 10.16036/j.issn.1000-2650.202104020
Abstract139)      PDF(pc) (1487KB)(72)       Save
【Objective】 The differences in organic acid metabolism between Ziziphus jujuba Mill. cv. ‘Luojiangtiaoyuanzao’ and Ziziphus acidojujuba Cheng et Liu cv. ‘Yuanxingxiaosuanzao’ fruits were studied. 【Method】 The different in organic acid contents and expression levels of metabolism-related genes in ‘Luojiangtiaoyuanzao’ and ‘Yuanxingxiaosuanzao’ fruits at different developmental stages were analyzed to determine the differences in organic acids. 【Result】 ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ contained malic, citric, tartaric, and succinic acids. A comparison showed that differences in the citric acid contents led to differences in the organic acid contents between the two cultivars. The expression levels of citric acid metabolism-related genes revealed that CS1, Aco2-1, and NAD-IDH were closely correlated with citric acid metabolism. Aco2-1, NAD-IDH1-2, and NAD-IDH5 were more highly expressed during the middle and late fruit developmental stages of ‘Luojiangtiaoyuanzao’ than ‘Yuanxingxiaosuanzao’. The expression levels of the malic acid metabolism-related genes FUM, PEPC4, and MDH1 were closely correlated with malic acid metabolism. However, FUM and PEPC4 were more highly expressed during the middle fruit developmental stage of ‘Luojiangtiaoyuanzao’ compared with ‘Yuanxingxiaosuanzao’, whereas MDH1 was more highly expressed during the late fruit developmental stage of ‘Luojiangtiaoyuanzao’. 【Conclusion】 The differential expression of Aco2-1 and NAD-IDH is responsible for the difference in organic acid content between ‘Yuanxingxiaosuanzao’ and ‘Luojiangtiaoyuanzao’ jujubes.
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Cloning and Expression Analysis of Melatonin Biosynthesis Gene AcTDCAcT5H and AcSNAT in Kiwifruit
LYU Xiaoyu, LIU Xinling, ZHANG Xuefeng, LIANG Dong, XIA Hui
Journal of Sichuan Agricultural University    2022, 40 (2): 191-197.   DOI: 10.16036/j.issn.1000-2650.202202052
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【Objective】 In order to deeply explore genes related to melatonin synthesis in kiwifruit and reveal melatonin function in kiwifruit under abiotic stress. 【Method】 AcTDCAcT5H and AcSNAT genes were cloned using RT-PCR and their expression in different tissues and under abiotic stress conditions were measured by qRT-PCR. 【Result】 The ORF regions of AcTDC、AcT5H and AcSNAT are 1 506、618 and 579 bp, coding 501、205 and 192 amino acid and the prediction of their subcellular localization are in mitochondria、endoplasmic reticulum and chloroplast,respectively. AcTDC only had expression in young fruits but not in roots、stems and leaves;the expression of AcT5H was comparatively high in young fruits,followed by stems, roots and leaves;the expression of AcSNAT was extremely high that dozens to hundreds of times higher than that of other tissues. When measuring their expression in leaves under abiotic stress,AcTDC still not expressed. AcT5H expression was down-regulated significantly after abscisic acid treatment and AcSNAT expression was down-regulated significantly after all treatments. 【Conclusion】 Proteins encoded by three genes are conservative and tissue-specific,providing theoretic basis for further study of melatonin resistance mechanism in kiwifruit.
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The Impact of Land Transfer on Farmers’ Household Consumption from the Perspective of Life Cycle
ZENG Yurou, HU Yuan, ZENG Weizhong
Journal of Sichuan Agricultural University    2022, 40 (2): 292-300.   DOI: 10.16036/j.issn.1000-2650.202112035
Abstract137)      PDF(pc) (1394KB)(68)       Save
【Objective】 This paper aims to study the impact of land transfer on Farmers’ household consumption. 【Method】 Based on the panel data of CHFS in 2013 and 2015, this study used the DID method is to empirically analyze the differential impact of land transfer-in and transfer-out on Farmers' household consumption in different household life cycles. 【Result】 ①Land transfer-in or transfer-out will have a positive impact on household consumption, and have a more significant impact on living, leisure and transfer expenditure; ②Land transfer has a greater impact on the household consumption level of farmers whose family life cycle is in the development stage and burden stage;③Land transfer-in mainly affects consumption by increasing family income and improving family risk preference, while land transfer-out affects consumption by increasing family income and saving. 【Conclusion】 While paying attention to the improvement of rural residents' consumption level, we should pay more attention to the improvement of rural residents' consumption structure.
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Effects of Transgenic cry1Ac Corns on the Soil Surface Arthropods Communities in Corn Fields
CHEN Hongxing, WANG Renjie, SHAO Chunrui, KUANG Zhongfen, GU Junjie
Journal of Sichuan Agricultural University    2022, 40 (1): 125-129.   DOI: 10.16036/j.issn.1000-2650.202107004
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【Objective】 This study is to assess the effects of transgenic cry1Ac maize BT799 on biodiversity of arthropod community, which may provide certain theory basis to biosafety evaluation of genetically modified organisms. 【Method】 In nature fields, the effects of transgenic cry1Ac corn on community of arthropods were examined using traps method over two years. The species and population of the most common arthropod groups were investigated regularly in 3 experimental treatments including transgenic maize BT799 plot, its non-transgenic parent Z58 plot and local corn variety Zhengdan 958 plot. The effects of transgenic maize on the biodiversity of arthropod community were studied by analyzing diversity index, simpson index and pielou index of the whole arthropod community. 【Result】 The investigation showed that there were no significant differences in common community indices in corn field. The dynamic changes of species richness, evenness, dominant concentration and diversity index of the transgenic cry1Ac corns were more gentle than those of the conventional corns. 【Conclusion】 The stability of surface arthropod community in the transgenic cry1Ac corns field was higher than that in the conventional corns field, and the transgenic cry1Ac corns had no significant effect on field surface.
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Study on Distribution of Nitrate Nitrogen in Soil under Drip Irrigation
SUN Fubin, YIN Juan, WEI Xiaodong, MA Zhenghu, SU Zhenjuan
Journal of Sichuan Agricultural University    2021, 39 (6): 807-813.   DOI: 10.16036/j.issn.1000-2650.2021.06.014
Abstract135)      PDF(pc) (4805KB)(86)       Save
【Objective】 This study aims to improve the water and fertilizer management level of Lycium barbarum field in the central arid region of Ningxia, and establish a more efficient water and fertilizer management mode, so as to better serve the development of local water-saving and high-efficiency irrigation agriculture. 【Method】 In this study, nitrogen concentration (0, 200, 500 mg/L), drip head flow (0.5, 0.75,1 L/h) and irrigation amount (3, 5 L) were used to study the variation of soil nitrate nitrogen content with the time of redistribution, drip head flow and nitrogen concentration.The law of migration in the radial and vertical directions. 【Result】 ①The nitrate nitrogen content in different soil depth (0-20 cm) decreased with the increase of radial distance; ②at different radial distances (0-20 cm), when the drip flow was 0.5 L/h, the nitrate nitrogen content first decreased and then increased with the increase of soil depth; when the drip flow was 0.75 L/h and 1 L/h, the nitrate nitrogen content first decreased, then increased and then decreased with the increase of soil depth; ③In different radial distance (0-20 cm), and the absence of nitrogen fertilizer (F1, 0 mg/L), nitrate nitrogen content increasing trend along with the increase of soil depth, nitrogen concentration is F2(200 mg/L), F3(500 mg/L), it along with the increase of soil depth increase with the decrease of the first; ④Redistribution time, drip flow rate and nitrogen concentration were significantly correlated with nitrate nitrogen content (P<0.01). 【Conclusion】 According to the comprehensive indexes, when the redistribution time is 5 d, the drip flow rate is 0.5 L/h, and the nitrogen concentration is about 500 mg/L, the nitrogen requirements of lycium barbarum field (sandy loam) under the condition of drip irrigation in this area can be met.
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Identification of Candidate QTL for Watermelon Seed Traits Based on GWAS
GAO Meiling, HU Chuangran, YUAN Chengzhi, GUO Yu, LIU Xiujie, LIU Jixiu, GAO Yue
Journal of Sichuan Agricultural University    2021, 39 (6): 721-728.   DOI: 10.16036/j.issn.1000-2650.2021.06.003
Abstract134)      PDF(pc) (2395KB)(84)       Save
【Objective】 To explore the genetic mechanism controlling the seed size of watermelon. 【Method】 Using 144 homozygous watermelon germplasms as test materials, the diversity index analysis,correlation analysis and genome-wide association analysis of seed size-related traits in multiple environments were carried out. 【Result】 There was a significant positive correlation between seed length,seed width and 100-seed weight of 144 germplasm resources; the diversity indexes were 4.69,4.78 and 4.80, respectively; the whole-genome association analysis detected 3 threshold values,the QTL regions of are located on chromosomes 4, 7 and 10,respectively. The QTL regions related to seed size traits on chromosome 7 have been detected in multiple environments. 【Conclusion】 Watermelon seed size is a complex quantitative trait,and its QTL is distributed on multiple chromosomes.
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Slope Differentiation Characteristics of Alpine Meadow Vegetation Community in Sygera Mountain, Tibet
SUN Kaige, YUAN Min, QU Xingle, LUO Daqing
Journal of Sichuan Agricultural University    2022, 40 (1): 90-99.   DOI: 10.16036/j.issn.1000-2650.202107001
Abstract133)      PDF(pc) (3168KB)(73)       Save
【Objective】 To understand the biomass characteristics of alpine meadow in the east and west slope of the Sygera Mountain. 【Method】 Through the study of alpine meadow area in the east and west slope of Sygera Mountain, research on the plant species composition, diversity characteristics, and biomass of the aforementioned area. 【Result】 53 species of Spermatophyta belonging to 37 genus and 22 families are found in the area mentioned above, from which Compositae Plants dominate, accounting for 18.87%; the Average Number of Shannon Wiener index, Margalef index and Pielou index are 3.23, 4.79 and 0.90 respectively, with significant facts that the aforementioned indexes in west slope are higher than that in the east and differences (P<0.05) are obvious; the total biomass is 443.24~626.14 g/m2, combined with the aboveground biomass, 443.24~626.14 g/m2, and underground biomass, 290.12~373.16 g/m2; the biomass in the east slope, mainly made up of Potentilla coriandrifolia and Polygonum macrophyllum cluster, is higher than that in the west, mainly composed of Polygonum viviparum and Kobresia macrantha cluster, alongside significant discrepancy (P<0.05). In the meanwhile, the biomass of dominant species in the east slope contributes more to the total biomass compared to that in the west. 【Conclusion】 The geographical elements in alpine meadow flora are complex with relatively similar characteristics in the temperate zone; the species distribution, in the west slope, is more balanced, and the species diversity is more abundant than that in the east slope. The results could provide the scientific foundation for the sustainable development of biomass in the Sygera Mountain’s alpine meadow area, and productivity in this area as well.
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Toxicity and Behavior Effects of the Extracts from Different Parts of Camptotheca acuminata on Solenopsis invicta Buren
YE Tao, YANG Jingmei, QIAN Yingjun, XI Yadong, LIU Guanghua
Journal of Sichuan Agricultural University    2021, 39 (6): 766-771.   DOI: 10.16036/j.issn.1000-2650.2021.06.008
Abstract133)      PDF(pc) (864KB)(77)       Save
【Objective】 The toxicity and the behavior of medium-sized workers of red imported fire ants have studied with Camptotheca acuminata extracts, providing the reference for using of plant source materials to control red imported fire ants and other major pests. 【Method】 The effects of different concentrations of extracts from Camptotheca acuminata leaves, stems, seeds, buds and also camptothecin solutions on the toxicity, aggregation rate, grasping rate, walking rate and climbing rate of Solenopsis invicta Buren workers were studied by water tube method. 【Result】 The extracts from different parts of Camptotheca acuminata showed some toxicity to the medium red fire ant workers compared with the control, and during the experiment, the mortality of red fire ants increased with time. The seed extract and 100 μg/mL camptothecin solution indicated the greatest toxicity, the mortality of red fire ants were 78.33% and 80.00% after 11 d, and the aggregation rate, grasping rate, walking rate and climbing rate of Solenopsis invicta Buren workers were 10.00% and 6.67%, 16.67% and 15.00%, 16.67% and 16.67%, 18.33% and 16.67%, respectively, with the same time. There was no significant difference between the treatments of seed extract and 100 μg/mL camptothecin solution, but significantly different compared with the results in control. 【Conclusion】 All the parts of Camptotheca acuminata showed toxicity and behavioral inhibition effects on the medium-sized workers,and seed has the optimal effects,maybe the potential to be developed into plant materials for control of red fire ants.
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Arthropods Community of Maize under Different Cultivation Patterns in Sichuan Province
ZHANG Xueyan, ZHANG Yue, CHEN Haonan, SONG Cancan, DENG Xiaoyue, WU Fei, DONG Yi, LI Qing, JIANG Chunxian
Journal of Sichuan Agricultural University    2021, 39 (6): 755-765.   DOI: 10.16036/j.issn.1000-2650.2021.06.007
Abstract132)      PDF(pc) (9564KB)(53)       Save
【Objective】 Clarify the characteristics of arthropod community in different cultivation patterns of maize in Sichuan Province. 【Method】 The species and quantity of arthropods in four cultivation patterns, including maize-soybean intercropping, maize-peanut intercropping, maize-sweet potato intercropping and net maize cultivation, were investigated. The composition, structure and community characteristic index of arthropod community in four cultivation patterns were analyzed, and the population densities of main pests and natural enemies of maize were compared. 【Result】 The maize field were found that the arthropod belonging to 5 classes, 18 orders, 94 families, 177 species. Among them, the number of species in the maize-peanut intercropping was the highest, with 117 species;followed by maize-soybean intercropping, maize-sweet potato intercropping and net maize cultivation, with 103, 101 and 78 species, respectively. Analyzed the arthropod community characteristic indexes of four cultivation patterns, the species richness of maize-peanut intercropping was the highest, which was 10.925 1;the Simpson diversity index, Shannon-Wiener diversity index, and Pielou evenness of maize-sweet potato were the highest, which were 0.166 3, 0.619 5 and 0.135 4, respectively. The dominant species of arthropod community in the four cultivation patterns were Rhopalosiphum maidis (Fitch), Empoasca flavescens (Fabricius), Harmonia axyridis (Pallas), Lasius niger (Linnaeus). Analyzed the population density of the main corn pests Rhopalosiphum maidis (Fitch), Pyrausta nubilalis (Hubern), Mythimna separata (Walker) during the main occurrence period and the occurrence dynamics of its natural enemies, it was found that compared with net maize cultivation, only maize-sweet potato intercropping among the three intercropping patterns had an inhibitory effect on the population density of Rhopalosiphum maidis (Fitch), and all three intercropping patterns had an inhibitory effect on the population density of Pyrausta nubilalis (Hubern), Mythimna separata (Walker). The density of natural enemies in three intercropping patterns was significantly higher than that of net maize cultivation. 【Conclusion】 Intercropping could increase the diversity index of the community, increase the species number and population density of natural enemies, and reduce the population density of Pyrausta nubilalis (Hubern) and Mythimna separata (Walker). Maize-sweet potato intercropping is the best cultivation to increase the diversity of arthropod community and reduce the population density of main pests in maize field.
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Biological Characteristics of Edwards tarda Isolated from A. davidianus and Resultant Pathological Lesions
XIANG Fei, GUO Xianghui, KANG Zhenya, FENG Yang, OUYANG Ping, CHEN Defang, HUANG Xiaoli, GENG Yi
Journal of Sichuan Agricultural University    2022, 40 (1): 111-117.   DOI: 10.16036/j.issn.1000-2650.202107003
Abstract129)      PDF(pc) (21914KB)(57)       Save
【Objective】 To clarify the pathogenic characteristics and pathological lesions of Andrias davidianus with enteritis and ascites of two farms. 【Method】 Bacterial isolation, morphological observation, biochemical determination, 16S rDNA sequence analysis, drug sensitivity test by K-B, virulence-related genes detection and histopathological observation after HE staining have been performed on diseased A. davidianus. 【Result】 Two dominant bacterial strains (XY01, XY02) were isolated from the liver, kidney, and ascites of the diseased A. davidianus, which were identified as Edwards tarda with six virulence genes (citC, fimA, gadB, katB, mukF, esrB). The drug susceptibility results showed that the isolates were resistant to amoxicillin, vancomycin and kanamycin, but were sensitive to florfenicol, gentamicin and polymyxin-B. Histopathologically, the infected A. davidianus showed systemic inflammatory reaction and multiple tissues and organs were damaged, especially for liver, spleen, kidney and intestine, which manifested as cell degeneration and necrosis, and infiltration of a large number of lymphocytes, macrophages and neutrophils. 【Conclusion】 The biological characteristics and the pathological characteristics were identified of E. tarda caused the disease in two farms, which provides reference for the diagnosis, prevention and treatment of E. tarda in A. davidianus.
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Distribution Characteristics of the Natural Forest's Damage Information in Western Sichuan Based on QucikBird Data
LAI Jiaming, LI Jie, YUE Mingdi, ZENG Fanming
Journal of Sichuan Agricultural University    2022, 40 (1): 83-89.   DOI: 10.16036/j.issn.1000-2650.202108025
Abstract129)      PDF(pc) (22741KB)(81)       Save
【Objective】 It provides basic data and theoretical reference for the sustainable management of natural forest resources in Western Sichuan forest area. 【Method】 Set the QuickBird image as the basic data, this paper uses the object-oriented multi-scale segmentation method to establish the classification recognition rule set and extract the various damage information of the natural forest based on the Congnition 8.64 remote sensing image processing program, and the distribution characteristics of the damage information are analyzed by the GIS technology. 【Result】 ① the main types of natural forest's damages in the research area are geological landslides, agricultural and animal husbandry damage, snow damage and wind damage. The areas of geological landslides and farming and animal husbandry damage are 6.53 hm2 and 60.34 hm2, 33 trees are damaged by snow and 29 trees are destroyed by wind;② the damage of landslide is most possibly to happen in the bare land,where the slope is bigger than 50°,the density of gully is bigger than 7 km/km2 and the distance is less than 100m away from the road.Grassland area,where the slope is within 20° and the distance is between 500 m and 1 000 m away from the habitations,has the damage of farming and animal husbandry most clearly.The wind damageis most likely to happen in the forest area,where the elevation is from 3 500 m to 3 800 m of the northwestern slope and the crown density is less than 0.5.The region where the elevation is more than 3 500 m in the northern slope and tree species is piceaasperata or Abies fabriis most likely to have the snow damage. 【Conclusion】 Besides,the research shows that it is technically feasible to extract the natural forest's damage information based on the high resolution remote sensing images.
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An Endophytic Fungus Strain Isolated from Gentiana macrophylla and Analysis on Its Biological Activity
LI Peihua, ZHAO Huan, WEI Shuhong, LIU Xiaohong, ZHOU Liqian, DUAN Dianyou, PENG Zhengsong
Journal of Sichuan Agricultural University    2022, 40 (1): 50-57.   DOI: 10.16036/j.issn.1000-2650.202010028
Abstract129)      PDF(pc) (13379KB)(52)       Save
【Objective】 An endophytic fungus was isolated from the leaves of Gentiana macrophylla,analyzing the biological activities of antioxidation, antibacterial biological activities etc. And this work would provide a basis for further research on the mechanism of action and its application value. 【Method】 An endophytic fungus was isolated from the leaves of G. macrophylla by tissue block culture method. Then,the fungal endophytic strain was identified by sequencing of internal transcribed spacer (ITS) regions of rDNA and 28S nuclear ribosomal large subunit rRNA gene (LSU),as well as morphological observation. PKS gene sequences of the endophytic fungi strain was detected via PCR amplification. The antioxidant activity of the Ethyl acetate (EtOAc) extracts the endophytic fungi was investigated by DPPH radical scavenging assay,and optimization of the carbon and nitrogen source was conducted using the one-factor-at-a-time approach. The antibacterial activity of crude extracts from the endophytic fungi was carried out against two bacterial strains:Staphylococcus aureus and Escherichia coli by oxford cup method. 【Result】 The alignment of the result sequence indicated the endophytic strain was identified as Alternaria alternata,which also conferred by its morphology. EtOAc extracts from the strain exhibited high DPPH radical scavenging activities,with IC50 value of 2.728 mg/mL. The optimum carbon and nitrogen source were maltose and nitrate of potash,respectively. Besides,crude extract from the strain also had antibacterial activity against S. aureus and E.coli. 【Conclution】 The results indicated the endophyte could be considered as a potent source of new antibacterial/antioxidant compounds.
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Maternal Genetic Diversity, Differentiation and Phylogenetic Analysis of Three Yak Populations in Qinghai, China
BAI Shirui, MA Zhijie, MEI Sa, CHEN Shengmei, CHEN Fuju, GUO Weixing
Journal of Sichuan Agricultural University    2022, 40 (5): 664-669.   DOI: 10.16036/j.issn.1000-2650.202207120
Abstract128)      PDF(pc) (2707KB)(37)       Save
【Objective】 The current study was conducted to investigate the maternal genetic diversity, differentiation, clustering relationship, and genetic background of three yak populations (i.e., Dari, Maqin, and Ganglong populations) in Golog Tibetan Autonomous Prefecture of Qinghai, China at the molecular level. 【Method】 The 638 bp mtDNA D-loop sequences of 33 Ganglong yaks were sequenced firstly and then analyzed with corresponding sequences from 37 Dari yaks and 32 Maqin yaks reported in GenBank. 【Result】 The results showed that a total of 32 haplotypes were identified according to the 638 bp nucleotide sequence comparisons, of which the Dari, Maqin and Ganglong yak populations had 8, 6, and 8 specific haplotypes, respectively. The total haplotype diversity and nucleotide diversity of three populations were 0.921±0.014 and 0.020±0.010, respectively. Among them, the value of haplotype diversity was the highest in Ganglong yak (0.951±0.019), middle in Dari yak (0.901±0.034), but lowest in Maqin yak (0.887±0.033). Analyses of genetic differentiation and gene flow showed a moderate genetic differentiation and poor gene exchanged between Ganglong population and Dari (Fst=0.118; Nm=1.869), Maqin (Fst=0.129; Nm=1.688) populations respectively, while a low genetic differentiation and frequent gene exchanged between Dari and Maqin (Fst=0.021; Nm=11.655) populations. Dari and Maqin populations clustered firstly in cluster analysis, and then gathered with Ganglong yak population. Maternal phylogenetic analysis revealed that each of the three yak populations represented two maternal lineages (I and II) with two maternal origins. 【Conclusion】 The three yak populations in Golog Prefecture, Qinghai, China all have unique maternal genetic information. Ganglong and Dari yak populations have richer maternal genetic diversity than that of Maqin population. The genetic differentiation degrees between Ganglong and Dari or Maqin yak populations are higher, while the differentiation level between Dari and Maqin yak populations is lower. Each yak population is composed of two maternal lineages, speculating that they all have two maternal origins.
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Effect of Different Processing Technology on the Quality of Bashanzao Purple Bud and Leaf Green Tea
LUO Jinlong, YANG Taiju, CHEN Shengxiang, ZHANG Keyi, LIU Guanqun, LI Pinwu, XU Yanyan
Journal of Sichuan Agricultural University    2022, 40 (2): 214-219.   DOI: 10.16036/j.issn.1000-2650.202103032
Abstract125)      PDF(pc) (8208KB)(53)       Save
【Objective】 This paper purposes to explore the best processing technology of green tea from fresh leaves of purple buds of bashanzao in summer. 【Method】 In this study,green tea was made from purple one bud with one leaf of bashanzao in summer according to the processes of roasted curly green tea,roasted green curly tea, baked curly green tea, needle shaped tea,orchid shaped tea and Phoenix shaped tea. 【Result】 The contents of tea polyphenols,amino acids,soluble sugar,water extract,caffeine,anthocyanin,chlorophyll and simple catechin are 18.68%,3.4%,4.81%,41.22%,4.15%,4.98 mg/g,1.47 mg/g and 4.15%,respectively. The sensory quality is the best. 【Conclusion】 The processing technology of baked curly green tea is the best one to process the purple new shoot with one leaf and one bud of bashanzao in summer.
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Changes of Hormones, Polyamines and C/N in Different Floral Sex Differentiation Stages of Zanthoxylum planispinum var. dingtanensis Flower Buds
ZHOU Chaobin, RAO Maoxia, XU Guonan, WANG Zhiqin, GONG Wei, YANG Yongjian, TIAN Zhengyou, ZENG Boping
Journal of Sichuan Agricultural University    2022, 40 (3): 387-394.   DOI: 10.16036/j.issn.1000-2650.202108024
Abstract125)      PDF(pc) (8821KB)(51)       Save
【Objective】 In recent years,a large number of male flowers of Zanthoxylum planispinum var. dingtanensis have appeared in Guanling county and Zhenfeng county of Guizhou province,indicating that the floral differentiation process has changed. At present,it is not clear how the physiological characteristics change during floral differentiation. Based on this,the changes of hormones,polyamines and C/N during floral sex differentiation of Z. planispinum var. dingtanensis were studied,which can provide theoretical basis for the mechanism and regulation of floral sex differentiation of the plant. 【Method】 In this manuscript,Z. planispinum var. dingtanensis derived from Guanling county of Guizhou province was taken as the study material,the contents of gibberellin (GA3),indole-3-acetic acid (IAA),ethylene (Eth),abscisic acid (ABA) and zeatin (ZR) of flower buds at different floral sex differentiation stages were determined by enzyme linked immunosorbent assay (ELISA). The contents of putrescine (Put),phenylethylamine (Pea),cadaverine (Cad),spermidine (Spd),spermidine (Spm),tryptamine (Try),histamine (His) and tyramine (Tyr) in flower buds were measured by high performance liquid chromatography (HPLC). The soluble sugar content was determined by anthrone colorimetry method,and the soluble protein content was measured by Coomassie bright blue method,the ratio of soluble sugar to soluble protein was considered the C/N. 【Result】 ①There were significant differences in IAA and ABA between different stages of floral sex differentiation of Z. planispinum var. dingtanensis, and they all showed the pattern of female flowers > male flowers > flower buds in inflorescence primordium differentiation phase,indicating that high IAA and ABA content promoted the development of female flowers. GA3 content in both male and female flowers was significantly higher than that in flower buds in inflorescence primordium differentiation phase,and there was no difference in GA3 content between male and female flowers,indicating that higher GA3 content is beneficial to the development of both female and male flowers. ②In this experiment,both Put and Pea were not detected,and the other six polyamines had significant differences in different stages of floral sex differentiation. With the development of floral sex differentiation,Cad was firstly decreased and then increased,while Spd,Try and His were continuously decreased,suggesting that Cad may be related to female flower development,while Spd,Try and His may be related to male flower development. Tyr was firstly increased and then decreased,and the Tyr content in male flowers was significantly higher than that in both female flowers and flower buds in inflorescence primordium differentiation phase,while Spm was detected only in female flowers,indicating that high Tyr content promoted the development of male flowers while high Spm content promoted the development of female flowers. ③With the process of floral sex differentiation,C/N increased first and then decreased,the C/N of both male and female flowers was significantly higher than that of flower buds in inflorescence primordium differentiation phase,furthermore,the C/N of male flowers was significantly higher than that of female flowers,indicating that high C/N promoted the development of male flowers. 【Conclusion】 High concentrations of IAA,ABA and Spm promoted the development of female flowers,while high concentrations of Tyr and C/N were beneficial to the development of male flowers,which provided a reference for artificial application of different concentrations of exogenous hormones,polyamines or mineral nutrients to regulate the floral sex differentiation of Z. planispinum var. dingtanensis in the future.
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