【Objective】 The current study was conducted to clarify the effect of the field configuration of ′increasing the number of seedlings per hill with reduced number of hills′ （ISRH） on the canopy structure and light energy distribution characteristics of indica hybrid rice in the southwest China,and provide theoretical and practical basis for the popularization and application of ISRH field configuration in southwest rice area. 【Method】 A single-factor randomized field experiment was conducted to study the effects of different field configurations on canopy structure,light energy distribution,and photosynthetic characteristics of rice at Wenjiang and Hanyuan in Sichuan Province. Two field configurations including the traditional high-density planting （THDP,row hole spacing 30.0 cm × 12.0 cm,1.5 seedlings per hill,single and double seedlings staggered transplanting） and increasing the number of seedlings per hill with reduced number of hills （ISRH,row hole spacing 30.0 cm × 24.0 cm,3 seedlings per hill） were set. 【Result】 ①Compared with THDP,ISRH significantly increased the number of tillers per hill and decreased the leaf inclination angle of the upper three leaves by 0.93 %-3.97 % at heading stage at both study sites,which significantly increased the leaf area and canopy amplitude of single hill by 80.39 % and 28.99%,respectively. ②ISRH improved the permeability of the middle and lower parts of the rice canopy,which contributed to 18.08%-42.55% and 5.49%-44.22% increase in light transmittance of the canopy from 3 cm to 40 cm at Wenjiang and from 3 cm to 100 cm at Hanyuan,respectively. This leaded to the 46.74% and 11.54%-75.63% significant increase the daily radiation and illumination at the bottom of the canopy after heding,respectively. ③Compared with THDP,ISRH effectively improved the photosynthetic capacity of the upper three leaves of rice. The net photosynthetic rates of flag leaf,second leaf and third leaf increased by 16.96%-30.33%,9.35%-16.62% and 12.93%-32.76%,respectively. 【Conclusion】 ISRH improved the canopy structure of Yixiangyou 2115,thereby enhancing the light transmittance and light energy supply at the middle and lower parts of the canopy,which contributed to greater net photosynthetic rate of the upper three leaves of rice.

【Objective】 This paper aims to investigate the relationship between farm size, land transfer and fertilizer reduction and efficiency. 【Method】 Based on the survey data of 4 745 rural households in China in 2017, the effects of farm size and land transfer on the fertilizer use intensity（FUI） and fertilizer use efficiency（FUE） were empirically tested using ordinary least squares and instrumental variable regression methods. 【Result】 The farm size has a significant negative impact on the FUI and a significant positive impact on the FUE. On average, every doubling of the farm size will reduce the FUI by 30% and increase the FUE by 14%. Land transfer has a significant positive effect on the FUI and no significant effect on the FUE. The FUI of farmers who have transferred into land is 14% higher than that of farmers who have not transferred into land. 【Conclusion】 Realizing moderate-scale operation by promoting land transfer can effectively promote fertilizer reduction and efficiency. Further guaranteeing the standardization of land transfer and the stability of land management rights will help to promote farmers who have transferred into land to reduce the FUI.

【Objective】 The theoretical research on connected topics of the urban-rural income gap can be enriched by the study of the effects of the digital economy, and it can also offer recommendations for reducing the urban-rural income gap. 【Method】 Using data from Chinese cities from 2010 to 2019, this paper conducts an empirical analysis of the mechanism of the effect of the growth of the digital economy on the income gap between urban and rural areas. It views the "Broadband China" plan as a sort of organic experiment in the growth of the digital economy. 【Result】 The income gap between urban and rural areas can be greatly reduced as the digital economy grows. It was concluded as a consequence, and even following the PSM-DID and placebo tests, that the expansion of the digital economy could significantly reduce the income gap between urban and rural regions. Through the analysis of regional heterogeneity, it is concluded that when there are differences in geographical location and degree of marketization, the impact of the digital economy on the urban-rural income gap is also different. The heterogeneity results show that the development of the digital economy has significantly reduced the urban-rural income gap in the central and western regions, and the reduction effect in the western region is greater than that in the central region, but not significant in the eastern region; Moreover, in areas with a high degree of marketization, the effect of reducing the income gap between urban and rural areas is more obvious. The mechanism test results show that the digital economy reduces the urban-rural income gap through technological innovation and digitization. 【Conclusion】 Based on the above analysis, we should continue to carry out the strategy of "Broadband China", play the leading role of demonstration cities, further narrow the urban-rural income gap, and achieve common prosperity.

【Objective】 This study aimed to investigate the effects of Pueraria polysaccharides on contents of inflammatory factors, antioxidant capacity, immune function, DAO activity and intestinal microflora of diarrheic calves. 【Method】 A total of 12 healthy calves （group C） with similar body weight and age and naturally occurring 12 calves with typical diarrhea symptoms （group T） from the large-scale farm were selected in the experiment. Group T calves were fed with Pueraria polysaccharides 400 mg/（kg-head-day） in milk for 5 days, but not in group C. The calves were marked T₁ on the day of diarrhea and after the 5th day of administration as group T₂. The indexes of blood inflammation, immunity, antioxidation, intestinal permeability, as well as the diversity and abundance of intestinal flora in group C on the first day, T₁ and T₂ were detected. 【Result】 ① Pueraria polysaccharide can promote the levels of IL-1β significantly decreased （P<0.01）, IL-2, TNF- α and DAO significantly decreased （P<0.05）, MDA content showed a significant decreasing trend （0.05≤P<0.10）, and TGF-β, GSH-Px and IgA significantly increased （P<0.01） in diarrheic calves. ② After treated with Pueraria polysaccharides, the relative abundance of Bacterioidetes, Actinobacteria, Bacteroides and Faecalibacterium were increased （P<0.05）; while the relative abundance of Proteobacteria and Clostridium_sensu_stricto_1 was decreased. 【Conclusion】 Pueraria polysaccharide can reduce inflammatory reaction of diarrheic calves, enhance the antioxidation capacity, improve the immune function and regulate intestinal permeability. Moreover, it can also promote the growth of intestinal beneficial bacteria and inhibit the proliferation of harmful bacteria, so as to treat calf diarrhea.

【Objective】 To investigate the effects of exogenous sucrose on photosynthetic characteristics,substance accumulation and yield formation of soybean leaves under different shade treatments. 【Method】 The experimental design of two-factor split-plot was adopted,using shade-tolerant soybean 'Nandou 25' as the material,the main factors were the shading period： the third section to the first flowering stage shading treatment （VS）,the initial flowering to the mature stage shading treatment （RS）,the non-shade treatment （NS）,the secondary factors were exogenous sucrose concentration：0% （CK）,1.0% （S1） and 1.5% （S2）,from 3 nodes （V3） to 5 nodes （V5）,sucrose was sprayed on both sides of soybean leaves every day. 【Result】 S2 significantly increased the chlorophyll content of upper and middle leaves of VS by 10.41% and 7.80%,and significantly increased the dry matter of upper and middle NS layers by 40.00% and 33.52%,respectively. S2 treatment can increase the photosynthetic rate of upper leaves in the early shade treatment,which is 13.30% higher than CK. S2 increased the yield of VS,RS and NS by 5.98%,44.82% and 13.10% respectively compared with CK. S1 increased the photosynthetic rate of the middle and lower leaves in the early shade treatment,which increased by 17.34% and 9.29%,respectively,compared with CK. 【Conclusion】 Exogenous sucrose can increase the chlorophyll content of soybean leaves,increase the leaf area,increase its photosynthetic rate,alleviate the weak light stress caused by shading,and improve the photosynthetic characteristics,so as to increase the yield,and the treatment with S2 concentration is the best.

【Objective】 This experiment was conducted to study the effects of xylanase on slaughter performance, apparent digestibility of nutrients and serum biochemical indexes of Tibetan sheep. 【Method】 Sixty 2-3 month-old Tibetan ram lambs with similar initial weight and good physical condition were selected and randomly divided into two groups. Each group was set with 5 replicates, 6 animals for each replicate. The control group was fed with a wheat-based diet without xylanase, while the test group was fed with a wheat-based diet supplemented with 0.2% xylanase. The whole experiment was divided into a pre-feeding period of 10 days and a trial period of 90 days. 【Result】 ①Compared with the control group, there was no significant difference in slaughter weight, carcass weight, slaughter rate and eye muscle area in the experimental group （P>0.05）. The weight of liver, omasum and abomasum in the experimental group was significantly higher than that in the control group （P<0.05）. ②The digestibility of crude ash, neutral detergent fiber and acid detergent fiber in the test group was significantly higher than that in the control group （P<0.05）. ③The glucose content in serum of the test group was significantly higher than that in the control group （P<0.05）. 【Conclusion】 The addition of 0.2% xylanase to the diet had no adverse effect on the slaughter performance of Tibetan sheep, but it could effectively inhibit the anti-nutritional factors in wheat flour to improve the apparent digestibility of nutrients in Tibetan sheep and make the diet improve the digestive rate in rumen and abomasum to stimulate the organ development. While all serum biochemical indexes were within the normal range without negative effects.

【Objective】 The current study was conducted to explore the changes of the dominant bacterial number after Bacillus licheniformis SCAU1602 feeding laying hens to reveal the genetic diversity and phylogeny of culturable bacteria isolated from the chicken feces, and to screen some beneficial bacteria. 【Method】 Two treatments were designed, T1： conventional feeding （the control group）; T2： feeding with SCAU1602 （≥1×10⁸ CFU/kg）. The numbers of mesophilic bacteria, thermotolerant bacteria, coliform, and lactic acid bacteria in fresh chicken feces at 0, 7, 14, 30 and 90 days were determined in the two groups, respectively. The genetic diversity and phylogeny of culturable mesophilic bacteria and thermotolerant bacteria from chicken feces on the 90th day were isolated and analyzed. The protease and amylase activity and the NH₄⁺-N producing ability of representative strains from chicken feces on the 90th day were measured. 【Result】 The results showed that after feeding SCAU1602, the number of coliforms was significantly decreased, and the number of mesophilic bacteria, thermotolerant bacteria and lactic acid bacteria was significantly increased in chicken manure. A total of 133 bacteria were isolated from chicken feces of the two treatments on the 90th day, and the genetic diversity and phylogeny were performed. The 62 bacteria were isolated from T1 belonged to 11 species. Among them, Aerococcus viridans was the predominant mesophilic bacteria and occupied 33.33%, and Bacillus subtilis was the predominant thermotolerant one and accounted for 46.15%. The 71 bacteria were isolated from T2 belonged to 13 species. Among them, Enterococcus lactis was the dominant mesophilic bacteria and accounted for 19.05%, while Bacillus licheniformis was the dominant thermotolerant bacteria and occupied 41.38%. Compared with T1, the proportion of bacteria producing protease and amylase in chicken feces in T2 was significantly increased by 76.13% and 45.57%, the number of bacteria with strong ammoniacal ability was decreased by 35.32%, and the content of NH₄⁺-N in chicken feces was significantly decreased by 58.72% （P<0.05）. 【Conclusion】 Dietary supplemented with Bacillus licheniformis SCAU1602 could colonize well in laying hens feces. After feeding, the genetic diversity of culturable bacteria, and the number of bacteria with protease-producing and amylase-producing abilities in chicken feces were found to increase. While, the number of ammoniacal producing bacteria was significantly decreased, revealing that B. licheniformis SCAU1602 had a certain probiotic capacity.

【Objective】 Identification and evolutionary analysis of conserved miRNAs expression in rabbit muscle was studied, with the purposes of providing a theoretical basisfor the further studies of the biological functions of conserved miRNAs in rabbit growth and development activities. 【Method】 MiRNA libraries of table rabbit Longissimus muscle tissue were sequenced and analyzed via using Solexa technology. 【Result】 The results showed that 521 conserved miRNA sequences belonging to 238 different miRNA families were identified by sequence alignment among the obtained RNA pure sequences. miR-1, miR-133, miR-206 and miR-499 muscle-specific miRNAs were expressed in muscle. Except miR-499, the three other muscle-specific miRNAs were highly expressed in tables of rabbit muscle. 10 animal-conserved miRNA families were lost in the muscle genomes of S86 specialized paternal lines. The target genes were significantly enriched in 13 signaling pathways, of which the Focal adhesion and PI3K-Akt signaling pathways were closely associated with muscle development. 【Conclusion】 The identified and predicted miRNAs and their target gene enrichment pathways affecting muscle development will contribute to the study of growth regulatory mechanisms in table rabbit, which accelerates the development of molecular markers that can be used for marker assisted selection.

【Objective】 The present study was conducted to investigate the effects of different forms of zinc oxide on intestinal morphology, the expression of tight junction protein gene in jejunum mucosa,intestinal chymase activity and cecal fermentation in meat rabbits. 【Method】 A total of 162 New Zealand white rabbits,weaned at an age of 35 d , were randomly divided into three groups, with 27 replicates in each group and 2 rabbits in each replicate. They were fed with a basal diet supplemented with feed grade zinc oxide （F-ZnO）, nano zinc oxide （N-ZnO） and coated zinc oxide （C-ZnO） at a zinc level of 120 mg/kg in the basic diet. During the trial period of 42 days, 6 rabbits in each group were selected for slaughter sampling at 21 d and 42 d respectively. 【Result】 ① Compared with F-ZnO, dietary N-ZnO and C-ZnO significantly increased duodenal villus height, decreased crypt depth and increased villus/crypt ratio （V/C） （P<0.05）. Compared with F-ZnO and C-ZnO, N-ZnO significantly increased the ileal villus height , decreased the crypt depth, and increased V/C at 21 days of test （P<0.05）.And the depth of jejunal recess was significantly decreased and V/C was increased at 42 days of test （P<0.05）. ② Compared with F-ZnO, both the genes expression of Claudin, ZO-1 and ZO-2 at 21 d and that of Occludin at 42 d in the jejunum mucosa of meat rabbits in N-ZnO group and C-ZnO group were significantly up-regulated （P<0.05）. ③ Compared with F-ZnO, N-ZnO significantly increased the cellulase activity of caecal chyme at 42 d （P<0.05）. There was no significant difference in the activities of lipase, amylase and trypsin in jejunum chyme, hemicellulase and pectinase in cecal chyme in rabbits among the three groups （P>0.05）, and N-ZnO and C-ZnO did not affect concentration of the total volatile fatty acids and the proportion of acetic acid, propionic acid and butyric acid in cecal chyme of rabbits （P>0.05）. 【Conclusion】 The addition of N-ZnO and C-ZnO can promote the morphological development of small intestine in meat rabbits, improve the tight connection of jejunum mucosa , and enhance the intestinal health of meat rabbits.

【Objective】 This study was conducted to uncover the development of Kirin chicken with slow or fast feather, to evaluate if Kirin chicken could be used in breeding sex-identification set line and excavate and utilize the local genetic resources. 【Method】 Bertalanffy, Gompertz and Logistic models were used to analyze the growth patterns of fast and slow feathered line of the Kirin chicken from the age of 1 to 19 weeks and explore the suitable growth model. 【Result】 For the males, the body weight was significantly different between fast and slow feathered chicken at the 1^st week （0.01<P<0.05）, but there were no significant differences at the remaining weeks. While for the females, the body weight of the slow-feathered chicken was significantly higher than that of the fast-feathered ones at the 3^rd week （P<0.01）, but the results were opposite at the 7^th week （P<0.01）. At the remaining time point, there were no significant differences. The keel bone length tended to be consistent in different strains including roosters and hens, but the shank length and circumference showed different at various periods. The fitness of the 3 models for body weight was very high （R²≥0.985）, among which the best one was Bernalanffy model. The fitness （R²） of the fast-feathered rooster and hen was 0.999 and 0.993, respectively, while the fitness （R²） was 0.994 for the slow feathered rooster and hens. The best fitted model for keel bone length and shank circumference was the Bertalanffy for the two types of feathered Kirin chicken. While Logistic was the best model for the shank length of the fast-feathered chicken and Gompertz was the optimal model for the shank length of the slow-feathered chicken. 【Conclusion】 There were differences existed between the two lines of Kirin chicken during their growth and development. The Bertalanffy model was the best for body weight, keel bone length and shank circumference, respectively, while Logistic and Gompertz were optimal for the shank length of the fast and slow feathered Kirin chicken. In production practice, the optimal feeding conditions should be provided at different stages to meet the nutritional requirements of chickens during their growth and development.

【Objective】 This study was aimed to investigate the sugar transport function of mannose-1-phosphate guanyltransferase （GMPP） in Puccinia striiformis f. sp. tritici （Pst）. 【Method】 Primers were designed to screen and amplify the GMPP gene（named PsMT1） based on the comparison of Pst transcriptome data with 17 Pst genomes sets in NCBI database, and the GMPP gene of Pst was cloned, named PsMT1. PsMT1 was expressed in the Saccharomyces cerevisiae mutant strain EBY.VW4000 and grew on media with hexose or sucrose as the sole carbon source. 【Result】 The cDNA of PsMT1 was 1 245 bp in length, encoding 414 amino acids, and the isoelectric point was 6.05. Phylogenetic analysis indicated that PsMT1 had the closest genetic relationship with GMPP of P. graminis f. sp. tritici. Heterologous expression of PsMT1 can restore the growth of the hexose transporter deficient mutant yeast EBY.VW4000 on medium with D-mannose as the sole carbon source. 【Conclusion】 PsMT1 was cloned and its heterologous expression product could transport mannose. The results obtained in this study can provide basis for the further study of interaction between Pst and wheat mediated by sugar transporter.

【Objective】 This paper aims to explore the influence of stand density on species diversity and biomass of understory vegetation and the correlation between them. 【Method】 In this study, the typical site method was used to investigate the Cupressus funebris plantations with five different forest densities （500, 650, 800, 950 and 1 100 tree/ hm; 2） in Yunding Mountain, Jintang County, Sichuan Province, to calculate the diversity and biomass of the understory species. 【Result】 The results showed that a total of 172 plant species were recorded, belonging to 137 genera of 78 families. With the increase of stand density, the diversity index of shrub layer showed a unimodal change of first increase and then decrease, basically reached the maximum at the density of 650 tree/hm; 2, while the diversity index of herbaceous layer mostly showed bimodal changes of first increase and then decrease and then increase and then decrease, with peak values at the densities of 650 tree/hm; 2 and 950 tree/hm; 2. The biomass of shrub and herbaceous layer reached the maximum at the density of 650 tree/hm; 2, and the aboveground biomass was greater than the underground biomass. The important values of shrub species were highly positively correlated with their biomass of each layer and the aboveground and whole plant biomass of the herbaceous layer, and the important values of the species in the herbaceous layer were highly positively correlated with their aboveground and whole plant biomass. 【Conclusion】 It is concluded that the cypress plantation can maintain high species diversity and biomass of understory vegetation at a density of 650 tree/hm; 2, which is conducive to the healthy development of understory vegetation.

【Objective】 Facing the new development stage of China's poverty alleviation strategy transformation, how to achieve long-term effective assistance for poverty alleviation farmers and reduce income inequality has become one of the important issues to be solved urgently for the comprehensive realization of common prosperity. 【Method】 Based on the data of China Family Panel Studies （CFPS）, the risk of returning to poverty and income inequality of poverty-stricken households were measured by multidimensional poverty vulnerability and relative deprivation index, and the "prevent the return to poverty effect" and "distribution effect" of Maintaining the Sustainability of Poverty Alleviation Policies were empirically tested by FRD method. 【Result】 It is found that the policies of Maintaining the Sustainability of Poverty Alleviation significantly reduces the multidimensional vulnerability to poverty and the relative sense of deprivation of the farmers out of poverty, indicating that persisting in Maintaining the Sustainability of Poverty Alleviation policies is conducive to the formation of a win-win situation of curbing the risk of returning to poverty and improving income distribution in the post-poverty era. Further research shows that the policies of Maintaining the Sustainability of Poverty Alleviation can significantly improve the labor participation and business income of households that do not rely on the government's guarantee of subsistence, inspire their mental state of through-labor-to-change-life. However, it will make the families who rely on the government to guarantee poverty alleviation have certain welfare dependence, thus weakening the long-term stability of poverty alleviation policy effect. 【Conclusion】 The government should strengthen the top-level design of Maintaining the Sustainability of Poverty Alleviation policies and take multiple measures to enhance the endogenous development momentum of poverty alleviation farmers, so as to realize the connection between poverty alleviation and rural revitalization and common prosperity.

【Objective】 Common prosperity is the essential feature of socialism and the essential requirement of Chinese path to modernization, and farmers are the key group in the process of common prosperity. 【Method】 On the basis of a full review of existing literature, this paper systematically analyzes and explains the promotion mechanism of farmers' common prosperity from three aspects： how to make it possible, how to make it difficult, and how to realize it. 【Result】 To promote the common prosperity of farmers, it is necessary to address "economic poverty" as the premise,"dist+ribution according to work" as the basis, and "give priority to the development of agriculture and rural areas and comprehensively implement rural revitalization" as the path to achieve comprehensive and free development of farmers. 【Conclusion】 The content of farmers' common prosperity can only be effectively implemented in the coordinated process of urban-rural integration development, high-quality development of the new rural collective economy, digital economic development opportunities, and comprehensive promotion of rural revitalization by focusing more on the "essence of income".

【Objective】 This study evaluated the nutritional components of the fruits of four high-quality jujube varieties in Xinjiang,aiming to improve the unitariness of jujube in China and provide solutions to meet the needs of diversified industrial development to provide ideas. 【Method】 Correlation analysis and principal component analysis were mainly used to analyze the quality differences of different varieties of jujube. 【Result】 ① There were significant differences in composition content between varieties,with ′Nanjianghong′ having the highest sugar content and total flavonoids content,′Zhongzao 1′ having slightly lower sugar content than ′Nanjianghong′ but the highest nutrient content,′Huizao′ with the highest VC and mineral element content,and ′Junzao′ with the highest acid content and active ingredient content. ② The results of correlation analysis showed that there were strong correlations among different flavor or nutrient components. For example,soluble sugar was negatively correlated with anthocyanin and carotenoid,titratable acid was positively correlated with anthocyanin and carotenoid,and total phenol was positively correlated with fructose and glucose. ③ After systematic comparison,it was found that the sugar-acid ratio of ′Zhongzao 1′ and ′Huizao′ is higher than that of ′Nanjianghong′,and ′Nanjianghong′ is higher than that of ′Junzao′,so the first three varieties are more sweet;the starch and crude fat content of ′Zhongzao 1′ and ′Huizao′ is slightly higher than that of ′Junzao′,which is significantly higher than that of ′Nanjianghong′;the calcium and iron content of ′Zhongzao 1′ and ′Huizao′ is higher than that of ′Nanjianghong′,which is significantly higher than that of ′Junzao′;but the total flavonoids,anthocyanin and carotenoid content of ′Nanjianghong′ and ′Junzao′ are significantly higher than that of ′Zhongzao 1′ and ′Huizao′. 【Conclusion】 Comprehensive analysis shows that the active ingredient of ′Nanjianghong′ is better than that of ′Zhongzao 1′,but the nutritional composition of ′Zhongzao 1′ is higher than that of ‘Nanjianghong’,the mineral elements of ′Zhongzao 1′ and ′Huizao′ are higher, and they are suitable for consumption and enhancing human body functions;′Junzao′ is the most acidic,but the active ingredient content is the highest,and the antioxidant,beauty and health care functions are stronger as ′Nanjianghong′,and these research results provide reference basis and theoretical support for the distribution of Zizyphus jujube dates medicinal food resources.

【Objective】 The regional heterogeneous characteristics and influencing factors of the coupling and coordination level of rural revitalization and high-quality development in China are studied, and scientific basis is provided for accelerating the high-quality development of agriculture and rural areas. 【Method】 Based on the entropy weight TOPSIS, grey correlation degree and coupling degree model to calculate the coupling degree of rural revitalization and high-quality development in 30 provinces （autonomous regions and municipalities） in China from 2010 to 2019, the standard deviation ellipse, spatial autocorrelation, σ convergence method and Dagum Gini coefficient methods were used to investigate the spatiotemporal dynamic evolution characteristics of the regional heterogeneity of the coupling degree, and it also used the spatial Dubin and OLS models to further identify the driving factors of the coupling degree in China and four major regions. 【Result】 ① The coupling degree of the whole country and the four major regions showed an increasing trend year by year, and there were spatial distribution characteristics of "high in the east and low in the west", and the coordination type jumped from primary coordination to intermediate coordination. The elliptical center of gravity of the coupling degree is always located in Henan Province, indicating that its location distribution characteristics and dynamic evolution trajectory are relatively stable. There is a significant spatial positive correlation in coupling, but the correlation degree is weak. Except for the small diffusion trend in the coupling degree in the western region, the rest of the regions showed a convergence trend. The spatial difference mainly comes from between regions, followed by regions, and the supervariable density is the smallest. ② From a national perspective, population density, economic development, consumption level and financial development will have a significant positive effect on the coupling development level, while human capital factors have a negative inhibitory effect, and the population density, economic development, financial development and technological development of neighboring areas will also have spatial spillover effects, and the improvement of the coupling degree of surrounding areas will also have a positive radiation effect on the region. From a regional perspective, the drivers exhibit "region-specific" characteristics. 【Conclusion】 Adhering to the principle of "national overall planning and adapting measures to local conditions",this study found sthat it is necessary to strengthen the leading role of the government's macroeconomic regulation and control, and also give play to the role of the driving factors of each region, and also optimize the spatial layout structure of the coupling degree, enhance the linkage between regions, promote coordinated regional development, and then accelerate the modernization of Chinese-style agriculture and rural areas.

【Objective】 This study aims to reveal the relationship between plant diversity and soil physicochemical properties in Pinus massoniana plantation under different gap sizes. 【Method】 In this study, five different gap sizes （50-100、100-150、200-250、400-450、600-650 m²） in a 25-year-old P. massoniana plantation were taken as research objects, were compared with pure stand of P. massoniana. This study investigated and analyzed the vegetation composition, species diversity and soil physicochemical properties under different gap sizes suing Redundancy analysis （RDA） to determine the relationship between soil factors and species diversity. 【Result】 ① The important values of native tree species, such as Quercus serrata, Acer davidii, Eurya japonica, and Mallotus tenuifolius, were at the forefront in different forest gap sizes. The increase in the gap size led to an increase in the number of shade-tolerant species, while the number of semi-shade-tolerant species remained stable and occupied an important position. ② The G4 （400-450 m²） gap size showed the highest species diversity, and there was a strong positive correlation between species richness and diversity index of shrub layer and soil physicochemical properties. ③ The soil physicochemical properties under the middle and large forest gaps were obviously improved compared with those under the small forest gaps. The soil contents under G4 gap size showed of the highest content of total potassium （45.41 mg/kg）, available potassium （191.29 mg/kg） and organic matter （68.42 mg/kg）. The content of available potassium was significantly higher than that of other gaps （P<0.05）. Soil organic matter, soil total nitrogen and soil total potassium were the main environmental factors that affect the distribution of species under the forest gaps of P. massoniana plantation. 【Conclusion】 The results showed that plant diversity could sustain and even improve soil fertility, and G4 gap size （400-450 m²） was the most beneficial to plant community regeneration and soil nutrient cycling in this area.

【Objective】 To study the relationship between different dosages of slow-release fertilizer and the biomass distribution and anisotropic growth of Fraxinus malacophylla seedlings, in order to understand the survival strategies and ecological functions of F. malacophylla under different soil nutrients. 【Method】 One-year-old F.malacophylla seedlings were used for the study, and four different slow-release fertilizer dosage treatments were set up： control F₀ （0 kg/m³）, minimum use F₁ （1 kg/m³）, second use F₂ （2 kg/m³） and maximum use F₃ （4 kg/m³）; By measuring their biomass, the biomass distribution pattern of F. malacophylla seedlings under different treatments was analyzed using dry matter growth analysis, and the anisotropic growth relationship between rootstock and leaves as well as the seedling height and ground diameter biomass were analyzed using anisotropic growth analysis. 【Result】 The distribution of biomass and anisotropic growth of F. malacophylla seedlings differed between treatments with different slow-release fertilizer rates. Rootstock and leaf biomass accumulation showed that the treatment with minimum dosage of F₁ had the best effect, followed by the second dosage of F₂ treatment, and the lowest effect in the maximum dosage of F₃ treatment, and all treatments showed significant differences compared with the control F₀ （P<0.05）; The distribution of biomass in all organs of F. malacophylla seedlings under all four slow-release fertilizer treatments showed that leaves>roots>stems, as the amount of fertilization increased, the proportion of leaf biomass increased and tended to allocate more biomass to the leaves. The leaf biomass and stem biomass grew at anisotropic rates under the minimum usage F₁ treatment, the other treatments were isotropic and favored leaf growth. The anisotropic growth relationship of the rootstock and leaf biomass of white gunner seedlings at different slow-release fertilizer rates was not identical, but both isotropic and anisotropic growth. 【Conclusion】 The four slow-release fertilizer dosages changed the pattern of biomass accumulation and distribution in white gunner seedlings, with rootstock and leaf biomass accumulation being most effective at the minimum application rate of F₁ （1 kg/m³） and with the largest proportion of leaf biomass; The anisotropic growth relationship was not unique across the four slow-release fertilizer rates, with both isotropic and anisotropic growth occurring, reflecting the growth strategies of F. malacophylla seedlings at different slow-release fertilizer rates.

【Objective】 As an important agricultural area in the upper reaches of the Yangtze River,purple soil area in the Jialing River Basin has serious soil erosion as the purple soil was frequently disturbed by humans. It is helpful for protection of ecological environment to investigate the status of heavy metal pollution and its relation with magnetic susceptibility and particle size of soils in the region. 【Method】 Heavy metal elements （Cr,Ni,Cu,Zn,As,Cd,Pb and Mn）,magnetic susceptibility and grain size composition of 78 topsoil samples in the urban and suburban areas of Nanchong City in the middle Jialing River,and their correlations were analyzed. 【Result】 The insignificance relationships between the magnetic susceptibility,frequency magnetic susceptibility and particle size,are different from the correlation from that of the obvious polluted soil or the soil formed in natural environment,indicating that the soil physical properties ascribed by natural development were changed by human activities,whereas the soil was not polluted. The soil magnetic particles were mainly affected by human activities,resulted in a low frequency susceptibility. The mass magnetic susceptibility was significantly positively correlated with the main pollution elements including Pb,Cu,and As. A significantly positive relationship was detected between the clay content and the elements of Cr,Ni,Cu,Zn and As,whereas the clay content was obvious negatively correlated with Cd. The particle size had no correlation with Pb. Cd,Cr and Ni contents of the soils were mainly controlled by natural factors and were not affected by human activities. 【Conclusion】 The correlation analysis between soil magnetic susceptibility,particle size characteristics and heavy metal content may be used as a method for identifying heavy metal pollution of soil environment.

【Objective】 This paper aims to explore the resources of cultivable endophytic bacteria and screen the life-promoting strains in Impatiens siculifer. 【Method】 The endophytic bacteria of wild Impatiens siculifer were isolated by three isolation methods and five different culture media, and the isolated endophytic bacteria were identified by 16S rRNA-gene sequences. The activities of ACC deaminase, synthesis of IAA, the abilities of dissolving phosphorus and producing iron carrier were investigated. 【Result】 A total of 39 endophytic bacteria were obtained, belonging to 5 orders, 7 families and 8 genera. The suspension method （X method） had the best separation effect, and a total of 6 genera were obtained. The best isolation medium was M-7 glycerol-asparagine medium （No.5 medium）, and 5 genera were isolated. Based on the testing results of growth-promoting abilities, it showed that all 39 endophytic bacteria had the ability to produce ACC deaminase, and the strain SWFU195 had the highest ability to produce ACC deaminase among them, which was 11.039 0 U/mg; 27 strains were capable of producing IAA; 24 strains had phosphorus solubilization ability; 28 strains had siderophore production capacity; Among 39 strains of endophytic bacteria, 14 strains of endophytic bacteria also had phosphorus soluble, produce ACC deaminase, synthesis of IAA and produce iron carrier simultaneously. 【Conclusion】 Rich endogenous bacterial resources and life-promoting strains were found in I. siculifer, which provided certain basic data and theoretical basis for its development and utilization and its growth-promoting endophytic bacteria strains.

【Objective】 The grassland ecological protection subsidy and incentive policy （hereinafter referred to as "grassland subsidy and incentive policy"） can effectively improve herders' lives, promote production transformation and protect the ecological environment. 【Method】 The fuzzy set qualitative comparative analysis （fsQCA） method was used to explore the factors influencing the satisfaction of herders in three typical communities of alpine meadow, alpine grassland and desert grassland with the current grassland compensation policy and its group effect. 【Result】 The condition groupings showed the characteristics of "different paths", with single-factor policy cognition being the most important driver; the condition groupings had common and complementary relationships, and cultivation policy and ecological cognition were universal; herders in desert grassland areas were less influenced by livelihood capital. The influence of capital is smaller. 【Conclusion】 It is necessary to develop differentiated implementation plans based on zone-specific measurements and local conditions, improve the efficiency of granting subsidies and awards, and explore diversified compensation methods. At the same time, a grassland ecological protection publicity network should be established to improve the ecological and policy awareness of herders.

【Objective】 This paper was conducted to study the antibacterial activities of metabolites of different culture media and incubation times of Trichoderma longibrachiatum SMF2 and T. harzianum T39 and extracts of different organic solvents on Fusarium, and to clarify the culture conditions and extraction methods of SMF2 and T39 bacteriostatic active substances. 【Method】 Fusarium oxysporum, F. Solani, F. decemcellulare, F. equiseti and F. Keratomoplasticum, which caused root rot disease on Sophora japonica Linn were used as the test target, and the inhibition rate and virulence were determined by mycelial growth rate method. 【Result】 The results showed that the PD culture medium of SMF2 and T39 had the strongest inhibitory effect against F. oxysporum. SMF2 culture medium with WBM as nutrient matrix cultured for 5 days,and T39 culture medium with PD nutrient matrix cultured for 7 days had the best bacteriostatic effect. N-butanol was the best extraction solvent of the bacteriostatic active substances of SMF2 and T39, and the step-by-step extraction N-butanol extract has a good antibacterial effect against F. oxysporum, and the EC₅₀ was only 187.39 and 193.60 mg/L. 【Conclusion】 This study provided a theoretical basis for further study of the extraction method of antibacterial active substances of Trichoderma and of the development of Trichoderma biocontrol preparations and lead compounds.

【Objective】 This study aims to determine the adsorption effect of biomass carbon loaded with FeOOH Rosa roxburghii residue on fluoride in water. 【Method】 A new type of biomass carbon FeOOH-BC was prepared from Rosa roxburghii residue by alcohol assisted hydrothermal method. The static adsorption experiment was carried out with fluoride ion in aqueous solution as the target pollutant to investigate the effects of solution pH,adsorbent dosage,adsorption time and initial concentration on fluoride ion adsorption. 【Result】 When the adsorption reaction conditions were set as： pH=6, T=25℃, the initial concentration of fluoride ion is 50 mg/L,and the dosage of adsorbent =0.05 g, and the shaking adsorption lasted for 24 hours, the removal rate of fluoride ion by FeOOH-BC reached 95.27%. 【Conclusion】 The introduction of FeOOH can greatly improve the adsorption performance of biomass carbon for fluoride ions mainly by means of multi-layer adsorption and chemical adsorption.

【Objective】 Basic leucine zipper （bZIP） proteins constitute a large family of transcription factors among plants,which play vital roles during plant development. the expression of 11 ZmbZIPs in different tissues or under various stresses in elite maize inbred line Zheng58 could provide reference for the functional study of ZmbZIP or for the resistance breeding. 【Method】 Using qRT-PCR technique, this paper detected the expression profiling of 11 ZmbZIPs of maize inbred line Zheng58 was detected under 200 mmol/L NaCl,20% PEG6000,4 ℃ low temperature and nitrogen dificiency stresses. 【Result】 Plylogenetic tree revealed that 11 ZmbZIPs can be subdivided into 3 subgroups,indicating their genetic relationships. RT-qPCR analysis showed that 11 ZmbZIPs exhibited different expression patterns in different tissues,indicating diverse roles during maize growth and development. Under simulated stress treatment,200 mmol/L NaCl,20% PEG6000,4 ℃ and nitrogen deficiency, the expression patterns of 11 ZmbZIPs were detected,and the results showed that 11 ZmbZIPs were regulated by 4 stress treatments,indicating it′s widely involved in stress signaling pathways. 【Conclusion】 The expression patterns of 11 ZmbZIPs differed in different tissues or in responsive to various stresses. The study can offer valuable information for further revealing its biological functions.

【Objective】 The current study aims to determine the in vitro total antioxidant capacity of yeast-fermented goat milk and its effect on the skin of aging mice. 【Method】 Forty healthy female mice were selected and randomly divided into 4 groups, blank control group, aging model group, pre-fermentation control group, and fermentation experimental group. Except for the blank control group, D-galactose was injected subcutaneously on the nape of the neck every day for 42 consecutive days to establish the aging model. During the modeling process, sterile water was applied to the back skin of the mice in the blank control group and the aging model group, the pre-fermentation control group was applied with unfermented goat milk, and the fermentation experimental group was applied with fermented goat milk. After 42 days, the water content, malonaldehyde （MDA） content, superoxide dismutase （SOD） activity and hydroxyl radical scavenging ability in skin tissue were measured. 【Result】 Compared with the blank control group, the skin moisture content, hydroxyl radical scavenging ability and SOD enzyme activity of the mice in the aging model group were significantly decreased （P<0.05）, and the MDA content was significantly increased （P<0.05）. Compared with the aging model group, the water content, hydroxyl radical scavenging rate and SOD enzyme activity in the skin of the mice in the fermentation experimental group were significantly increased （P<0.05）, and the MDA content was significantly decreased （P<0.05）. 【Conclusion】 The experiment showed that fermented goat milk has a certain effect on delaying skin aging.

【Objective】 To provide the scientific basis for mechanized grain harvesting of summer maize in Sichuan,the current study was conducted to investigate the characteristics and relationship of grain filling and dewatering were studied in this paper. 【Methods】 In 2019 and 2020, the characteristic parameters of grouting and dehydration of six maize varieties were determined respectively. 【Result】 The results showed that the accumulated temperature required for grain filling was 1 119.1-1 754.9 ℃,with an average of 1 544.8 ℃. The accumulated temperature required to reduce the water content to 28% was 1 359.9-2 133.1 ℃,with an average of 1 612.6 ℃. 【Conclusion】 The grain filling rate and dehydration rate of maize showed no difference among years,but significant difference among varieties. Correlation analysis showed that grain filling rate showed significantly negative correlation with dehydration rate and water content at the physiological maturity stage. Selecting varieties with faster grain filling and dehydration was the key to reduce grain water content at the physiological maturity stage of Sichuan summer maize.

【Objective】 The physical characteristics and seed vigor of hybrids are important to measure the quality of hybrids. The study on the physical characteristics and seed vigor changes of hybrids with different maturity is helpful to guide the production of high quality hybrids. 【Method】 The changes of four physical characteristics （grain length,grain width,100 grain weight and proportion） and vigor of Zhenghong 431 seeds with different maturity were studied in Chengdu,Sichuan （20-55 days after pollination） and Xichang,Sichuan （20-75 days after pollination）. 【Result】 The grain length,grain width,100 grain weight and proportion of Zhenghong 431 increased gradually with the maturity,and finally became stable. Among them,the physical characteristics of Zhenghong 431 seeds in Chengdu tended to be stable at the 45th day after pollination,while at the 55th day in Xichang. Through the standard germination test and accelerated aging test,we found that the seed vigor of Zhenghong 431 in Chengdu reached the peak at the 45th day after pollination,while at the 60th day Xichang. 【Conclusion】 When maize hybrid-seeds were produced in Xichang and Chengdu bases of Sichuan province, the harvest was better at 60 and 45 days after pollination,respectively. The seed quality of Zhenghong 431 in Xichang was better than that in Chengdu.

【Objective】 To quickly and accurately detect crop pests and diseases in dense shading environments to meet the needs of large-scale and high-efficiency identification. The study proposes an improved RetinaNet crop pest detection model. 【Method】 Combines with DenseNet, the RetinaNet feature extraction network was modified to enhance feature reuse, improve the recognition rate of the deep network model for crop pests, and identify the species of pests and diseases accurately and quickly. Its non-great suppression algorithm adopts the DIoU strategy, which effectively reduces the leakage rate of crop pests in the case of dense targets and improves the detection accuracy. 【Result】 The experimental results show that the improved algorithm model has high detection accuracy and good real-time performance, and has good adaptability to the dense crop shading situation, and the mmAP reaches 49.8%. 【Conclusion】 Applying the model to the complex crop growth environment can effectively improve the intelligent detection of pests and diseases.

【Objective】 The present study was conducted to understand the distribution of photosynthetic carbon assimilation products by conifer species in different organs and the amount of input in the soil. 【Method】 Taking Picea asperata and Larix masteriana as the research objects, four ¹³C isotope pulse markers were performed on seedlings in the growth stage by combining potting experiments with pulse labeling, in order to reveal the distribution of photosynthetic carbon assimilation products of P. asperata and L. masteriana in organ-soil. 【Result】 After labeling, the δ¹³C values in each organ and soil of two tree species were significantly different from their initial values, and ¹³C enrichment ability of two species showed as L. masteriana > P. asperata. The δ¹³C values of root, branch, leaf and soil in L. masteriana ranged from 25.33‰ to 171.64‰, 36.68‰ to 206.84‰, 79.40‰ to 230.91‰, and -16.49‰ to -11.28‰, respectively, which were specifically manifested as leaf > branches> roots> soil. And the δ¹³C values of root, branch, leaf and soil in P. asperata ranged from -23.02‰ to 63.97‰, -23.72‰ to 18.52‰, -20.09‰ to 14.09‰, and -20.27‰ to -15.71‰, respectively, which were specifically manifested as roots > leaves > branches > soil. 【Conclusion】 Under the labeling of ¹³C pulse marking method, the enrichment of L. masteriana ¹³C is higher than that of P. asperata. The carbon fixation of L. masteriana leaves is higher than that of P. asperata leaves, while the concentration of ¹³C in P. asperata roots is relatively higher than that of L. masteriana. These results show that pulse labeling is effective in exploring the distribution of photosynthetic products in organ-soil at the seedling stage for conifer species, which provides certain basic data and technical support for the application of stable isotope pulse labeling in plant physiology and ecology.

【Objective】 The focus of China's poverty alleviation strategy has shifted from the overall goal of poverty alleviation to the solution of relative poverty, to meet the refined and personalized needs of poverty governance transformation, to consolidate and expand the effective connection between poverty alleviation achievements and rural revitalization, and to systematically establish the logic and mechanism of relative poverty governance to match the rural revitalization and development plan, so as to provide theoretical guidance and value reference for comprehensively promoting rural revitalization. 【Method】 Based on the analysis of the connotation, characteristics and governance strategy of relative poverty, based on the principle of systematic analysis, the empirical study of local relative poverty governance in Hebei was conducted to explain the logic and mechanism of relative poverty governance from the perspective of rural revitalization strategy. 【Result】 Due to the differences in the basic conditions for promoting rural vitalization, regional governance emphases were different, but there was overlapping consensus on the logic of relative poverty governance. Construct long-term relative poverty governance mechanism based on the logic of relative poverty governance. 【Conclusion】 The logic of “one core and five governance” for relative poverty is extracted with the party's political leadership as the core, industrial technology governance, cultural internal governance, social co-governance, good governance of people's livelihood and ecological rule of law as the means. Construct a relative poverty governance mechanism from six aspects： organizational promotion, scientific and technological innovation, endogenous motivation, collaborative governance, income growth and legal protection.

【Objective】 Based on previous proteomics results, proteins PcMCM3, PcRPS27A and PcMCM5, which related to structure and function of hemocyte membrane, were screened from the down-regulated proteins. After that, the gene interference system was constructed, and then the effects of the 3 proteins in toxicity of PA （pedunsaponin A） to P. canaliculata were tested. 【Method】 Analyzing the results of phylogenetic tree and spatiotemporal expression, screening the optimal siRNA for interference, and detecting the mortality of P. canaliculata after interference. 【Result】 Results of phylogenetic tree showed that PcMCM3, PcRPS27A and PcMCM5 had higher genetic relationships with （Aplysia californica, XP_005107582.1）, （Octopus bimaculoides, XP_014787008.1）, （Biomphalaria glabrata, XP_013089107.1） and other species. Results of Spatio-temporal expression showed that the mRNA expression levels of the 3 proteins significantly decreased after PA treated, which were consistent with the results of previous proteomics studies. After siRNA screening, siRNAs with interference efficiency greater than 90% were obtained and further used for verification tests. By comparing the interference target protein gene treated with PA with those P. canaliculata without interference, it was found that the mortality of P. canaliculata had no difference between the treated and untreated groups. 【Conclusion】 The results showed that this candidate protein didn't affect the toxic effect of PA on P. canaliculata.

【Objective】 This paper aims to identify and analyze the members of Hsf transcription factor family in Brassica juncea L. genome,hoping to provide a theoretical basis for the functional research of Brassica juncea L. Hsf gene and genetic improvement to improve stress resistance. 【Method】 Bioinformatics methods were used to analyze the functional structure,conserved motifs,promoter cis acting elements,phylogeny,collinearity of BjuHsf family members,and RNA-seq was also used to verify the gene expression of BjuHsf under low temperature stress. 【Result】 Seventy-one members of the Hsf gene family were identified in the Brassica juncea L. genome,distributed on eighteen chromosomes and grouped into three subfamilies. all BjuHsf proteins contain DBD and HR-A/B domains. The BjuHsf promoter region contains cis-acting elements related to stress,hormones,and growth and development. The phylogenetic and collinearity analysis indicated that the Hsf family members of Brassica juncea L. were more closely related to those of chinese cabbage. Under low temperature stress,BjuHsf gene expression analysis showed that eight BjuHsf genes were significantly up-regulated. 【Conclusion】 These differentially expressed BjuHsf genes are related to cold tolerance of Brassica juncea L. and can be used as candidate genes for genetic improvement of cold tolerance of Brassica juncea L.

【Objective】 This paper aims to explore the effects of different temperature and salinity on R_O（on oxygen consumption rate） and R_N（ammonia excretion rate） of Paphia textile. It provides a basis for the breeding of this shellfish. 【Method】 The effects of temperature （10,15,20,25,30） and salinity （15,20,25,30 ℃） on R_O and R_N of P. textile were studied by experimental ecological method. 【Result】 At the temperature of 10-30 ℃, with the increase of water temperature, the R_O of P. textile gradually increased, while the R_N first increased and then decreased, reaching the maximum at 20 ℃; When the salinity is 15-30, with the increase of salinity, the R_O and R_N of P. textile gradually increase. When the salinity is 25, the R_N decreases slightly. In the range of salinity 29 and water temperature 10-30 ℃, the O∶N value of P. textile is 16.320-71.046, with an average value of 31.692. When the water temperature is 24 ℃ and the salinity is 15-30, the O∶N value is 10.459-32.974, and the average value is 19.060. Under the experimental conditions, according to the experimental data, we got equation between R_O and temperature： y=0.027 7x+0.130 1, R²=0.985 2; between R_N and temperature： y=-0.000 2x²+0.007 8x-0.045 4, R²=0.808 4; R_O and salinity： y=0.047 2x-0.637 4, R²=0.922; between R_N and salinity： y=-0.000 2x²+0.010 2x-0.117 9, R²=0.767 3; O∶N and temperature： y=0.296 5x²-9.898x+96.218, R²=0.874 1; O∶N and salinity： y=0.233 4x²-11.581x+154.21, R²=1. 【Conclusion】 The optimum temperature and salinity for the survival of P. textile are 25 ℃ and 25, respectively. The results provide a reference for its artificial breeding and large-scale breeding management.

【Objective】 This study aims to investigate the effects of different fertilization treatments on microbial mass, soil enzyme activity and physiology of grapes in the rhizosphere. 【Method】 The grape seedlings of three varieties of Nina Queen, Summer Black and Shine Muscat have been selected as test materials, each of which has been adopted with three fertilization methods respectively：application of well-rotted organic fertilizer （CK group）, rotten organic fertilizer+microbial fertilizer （compound microbial fertilizer group） and rotted organic fertilizer+bamboo fiber microbial fertilizer （bamboo fiber fertilizer group）. Rhizosphere soil has been randomly collected to determine soil nitrogen, phosphorus and potassium content, microbial biomass and soil enzyme activity, and chlorophyll content and relative leaf water content of grape seedlings. 【Result】 Both bacterial fertilizers can significantly increase the contents of available nitrogen, available phosphorus and available potassium in the rhizosphere soil of the three varieties of grapes, and the bamboo fiber fertilizer group was significantly higher than that in the compound microbial fertilizer group. Both kinds of bacterial fertilizers can significantly increase the microbial biomass of grape rhizosphere, and the number of bacteria in the bamboo fiber fertilizer group was significantly higher than that in the compound microbial fertilizer group, while there was no significant difference between fungi and actinomycetes. The activities of urease, sucrase and phosphatase in soil under bamboo fiber fertilizer were greatly increased, and the compound microbial fertilizer group had an increase, but the increase was relatively small, and the effect of the two bacterial fertilizers on soil catalase activity was small, but there was still a slight increase, and the bamboo fiber fertilizer was higher than that of the compound microbial fertilizer. Both bacterial fertilizers can significantly increase the chlorophyll content and relative water content of grape leaves, and the bamboo fiber fertilizer group was slightly higher than that of the compound microbial fertilizer group. 【Conclusion】 The application of bamboo fiber fertilizer can effectively solve the problem of adaptability of plant rhizosphere microorganisms in soil, and significantly increase the microbial biomass of grape rhizosphere soil. It increases the activity of urease, catalase, sucrase and phosphatase in rhizosphere soil, promotes the transformation of nutrients in soil matrix, increases the content of soil nutrients in nitrogen, phosphorus and potassium, and increases the chlorophyll content and relative water content of grape seedlings.

【Objective】 This study aims to investigate and select the variety with the best quality and antioxidant capacity from 9 varieties of citrus. 【Method】 High performance liquid chromatography was used to determine the compositions and contents of sugars and acids in the fruits. The fruit quality was comprehensively analyzed and compared by employing principal component analysis. The radical scavenging activities were determined by ABTS, DPPH and FRAP. Correlation analysis between fruit quality and antioxidant capacity was also performed. 【Result】 The results showed that total sugar content ranges from 53.98 to 152.88 mg/g,total acid content varies from 6.06 to 16.03 mg/g, and the acid ratio varies from 3.88 to 22.71,among which W. murcott is the highest. The principal component analysis of fruit quality showed that the comprehensive score of fruit quality varies from -3.25 to 4.93,among which Xiangfeicheng is the highest while Huangguogan is the lowest. And the ABTS of nine citrus varies from 0.18 to 0.79 mmol TE/g. The range of DPPH is 0.069 5~0.148 2 mmol TE/g,that of FRAP is 1.16~1.67 mmol TE/g,and that of comprehensive antioxidant index is 44.62~79.69. 【Conclusion】 Among the 9 citrus varieties,the comprehensive fruit quality of Xiangfeicheng is the best,while Valencia sweet orange has the strongest antioxidant capacity.

【Objective】 This paper aims to clarify the potential distribution of Spodoptera frugiperda in Sichuan Province and provide reference for monitoring, early warning and prevention S. frugiperda in Sichuan. 【Method】 This study first optimized the parameters of the MaxEnt model. Then, the optimized MaxEnt model and the latest version of WorldClim were used to predict the potential distribution of S. frugiperda in Sichuan under different climate backgrounds at present and in the future. 【Result】 The potential distribution area of S. frugiperda in Sichuan is 25.166 4 million hm², accounting for 51.18% of the total area of Sichuan. Most areas in Sichuan are potential distribution areas for S. frugiperda, except for the Tibetan Autonomous Prefecture of Garzê and Tibetan Qiang Autonomous Prefecture of Ngawa on the Western Sichuan Plateau and parts of Liangshan Yi Autonomous Prefecture in southwestern Sichuan. Based on the overall distribution trend, the suitability increases from west to east. Under future climate change scenarios, the range of the potential distribution area for S. frugiperda will expand. The area of high suitability will increase by 9.00%~37.67% of the current area; the areas of medium and low suitability will decrease by 13.29%~52.21% and 3.44%~26.08%, respectively; and the unsuitable area will decrease by 1.60%~16.72%. 【Conclusion】 Sichuan Province has a large area of potential distribution for S. frugiperda. Under the future climate conditions, the potential distribution of S. frugiperda in Sichuan will expand westward and northward.

【Objective】 This study aimed to explore whether a single above-ground host plant can slow down the decomposition of soil organic matter by reducing the diversity of underground mycorrhizal fungi, and ultimately reducing the availability of soil nutrients. 【Method】 In view of this, ECM fungal communities with different gradients of diversity （1, 2, 4, 8 species of ectomycorrhizal fungi） were inoculated into the sterilized soil of Picea asperata seedlings （collected from the subalpine P. asperata plantation in southwest China）, and the control （CK） was also established. After one year, the physical and biochemical properties of the rhizosphere soil were determined. 【Result】 The results showed that the pH value of soil inoculated with four ECM fungi （5.44） was significantly higher than that of control and other treatments （5.21-5.30）. The contents of soil organic matter and organic carbon decreased with the increase of ECM fungal diversity （organic matter, 37.62-22.81 g/kg; organic carbon, 21.82-13.23 g/kg）. The contents of organic matter and organic carbon in the control were significantly higher than those in the eight ectomycorrhizal fungal inoculation groups （P < 0.05）. Inoculation of ectomycorrhizal fungi with different gradients of diversity had no effect on soil total nitrogen or total phosphorus contents. The contents of soil nitrate nitrogen （44.81-64.56 mg/kg）, inorganic nitrogen （68.45-87.23 mg/kg）, and total dissolved nitrogen （341.28-483.72 mg/kg） increased with the increase in the ECM fungal diversity, except for the inoculation of four species of ectomycorrhizal fungi （nitrate nitrogen, 29.68 mg/kg; inorganic nitrogen, 49.65 mg/kg; total dissolved nitrogen, 181.18 mg/kg）, suggesting that these four fungal combinations could facilitate the host-plant to acquire nitrogen, but are not conducive to the activation of nitrogen components from organic matter. 【Conclusion】 The higher the diversity of the ectomycorrhizal fungal community, the stronger the effect on the decomposition of organic matter and the improvement of nitrogen availability. The results can provide data support and a theoretical basis for the role of mycorrhizal fungal diversity in soil ecological function in the southwest Subalpine region.

【Objective】 The current study was conducted to compare and analyze the difference of quality traits between Sichuan brewing and non-brewing wheat cultivars in Sichuan aiming at providing theoretical basis for breeding brewing wheat. 【Method】 Three brewing and three non-brewing wheat varieties each were used as test materials. Starch content and crude protein contents were determined by employing starch detection kit method and Kjeldahl nitrogen determination method,and hardness index and other quality traits were also detected by using grain hardness tester and near-infrared spectrometer. 【Result】 The average total starch content of the three brewing wheat was more than 60%,and the average amylopectin content accounted for more than 70% of the total starch content,which was significantly higher than that of the three non-brewing wheat. Their average crude protein content was less than 13%, which was only significantly different from that of Neimai 101,but the hardness index and sedimentation value were significantly lower than those of the three non-brewing wheat. In addition,the water absorption,stable time and wet gluten content were significantly lower than those of some non-brewing wheat. These results indicated that all three brewing wheat cultivars belonged to weak gluten wheat. 【Conclusion】 The results showed that high quality weak gluten wheat with crude protein content less than 13% was suitable for selection as brewing wheat variety.

【Objective】 This study was conducted to clone the CDS sequence of prolactin receptor gene （PRLR） of Duolang sheep and detect the expression difference of PRLR gene before and after the initial estrus in different tissues, so as to provide reference for the main role of PRLR gene in the initiation of the initial estrus. 【Method】 RT-PCR technology and relevant biological information software were used to predict the structure and function of the encoded protein, and qPCR was used to detect the expression level of PRLR gene in each tissue of Duolang sheep before and after the first estrus stage. 【Result】 The CDs sequence of PRLR gene of Duolang sheep was 1 746 bp in length. PRLR gene was expressed in all five tissues of Duolang sheep at three stages. The expression level of PRLR gene in pituitary tissues of Duolang sheep before estrus was significantly higher than that in other tissues （P<0.05）. The expression level of PRLR gene in pituitary tissues was still significantly higher than that in the other four tissues （P<0.05）, and the expression level of PRLR gene in hypothalamus and pituitary tissues was significantly higher than that in the other three tissues after the initial estrus （P<0.05）. The expression of PRLR gene was increased in pituitary and oviduct tissues during the initial estrus period, and was significantly higher after the initial estrus period than before and after the initial estrus period （P<0.05）. 【Conclusion】 These results suggest that PRLR gene may be involved in the regulation of estrus initiation in Duolang sheep.

【Objective】 This study purposes to investigate the molecular regulation mechanism of preservation under the conditions of 1-MCP treatment combined with refrigeration （1-4 ℃） storage of post-harvest ′Hong yang′ kiwifruit. 【Method】 In this experiment,the fruits were used as the material,and 1-MCP and refrigeration were used to observe the change in the physiological effects of fruits during storage. 【Result】 The results showed that 1-MCP treatment combined with cold storage slowed down the decrease of kiwi fruit hardness,inhibited the degradation of TSS,titratable acid,VC and anthocyanin,slowed down the accumulation of MDA,maintained the vitality of SOD,and reduced the ethylene release to delay the arrival of the peak of ethylene release. By performing comparative transcriptomic analysis using RNA-seq technology,a total of 2 380 differentially expressed genes were screened,including 1 503 upregulated and 877 downregulated expressed genes. The GO enrichment analysis indicated that the differentially expressed genes were significantly enriched in the functional categories of cellular components,molecular function,and biological processes,including peptide biosynthesis and metabolism,amide biosynthesis,protein transport,and defense response. KEGG enrichment analysis showed that the upregulated genes were mainly enriched in the plant hormone signaling transduction pathway,cysteine and methionine metabolism pathways,and antioxidant-related pathways such as phenylalanine metabolism and glutathione metabolism. It is speculated that the increased expression of methionine metabolism is one of the main reasons for reducing ethylene synthesis. The downregulation was mainly enriched in pyruvate metabolism pathways,chlorophyll metabolism,nicotinic niacin and nicotinamide metabolism,and proteasome metabolism pathway and other pathways that affect the intensity of respiration. 【Conclusion】 1-MCP combined with cold treatment of ′Hong yang′ kiwifruit,through improving the fruit antioxidant-related gene expression,reduce the ethylene synthesis precursor and inhibiting the respiration-related gene expression,so as to achieve the preservation effect of delaying the fruit aging. This study revealed the preservation mechanism of 1-MCP from the molecular level and provided a theoretical reference for the preservation research of ′Hong yang′ kiwifruit.

【Objective】 The study purposes to investigate the optimal ratios of ammonium to nitrate suitable for the main coniferous tree, Cunninghamia lanceolate, and broad-leaved tree, Schima superba, so as to improve the productivity and nitrogen use efficiency for the broadleaf-conifer mixed forests in subtropical zone. 【Method】 In this study, seedlings were cultured under five ratios （10∶0, 0∶10, 7∶3, 3∶7, 5∶5） of NH₄⁺-N to NO₃^--N and the photosynthetic characteristics, seedling growth, biomass and corresponding distribution were measured and compared for the two species. 【Result】 The ammonium-nitrate ratio of 5∶5, 7∶3 and 3∶7 in treatments increased the net photosynthetic rate, transpiration rate, stomatal conductance, biomass and seedling height of both C. lanceolata and S. superba, compared to the ratio of 10∶0 and 0∶10 in treatments of single N form. Higher ammonium concentration significantly increased the net photosynthetic rate, seedling height, ground diameter and biomass of C. lanceolata, while treatments of higher nitrate N concentration increased the net photosynthetic rate, seedling height, ground diameter and biomass of S. superba. Higher ammonium concentration reduced the root-shoot ratio of C. lanceolata, indicating promoting the growth of C. lanceolata aboveground part. Higher nitrate N concentration reduced the root-shoot ratio of S. superba, indicating promoting the growth of S. superba aboveground part. 【Conclusion】 Different nitrogen forms and ratios significantly affected the photosynthesis and growth of C. lanceolata and S. superba. The mixed treatment with the same proportion of ammonium and nitrate is most beneficial to the photosynthesis, seedling height growth and biomass accumulation of C. lanceolata, and the mixed treatment with higher concentration of nitrate N is most beneficial to the photosynthesis and biomass accumulation of S. superba. In the seedling cultivation and mixed afforestation with the two tree species, we can apply ammonium N to C. lanceolata and nitrate N to S. superba according to the situation of N in the soil.

【Objective】 In order to understand the effects of seed soaking with the melatonin on seed germination and seedling physiology of Keteleeria everyniana, this study implemented seed soaking trial with different concentrations of melatonin solution to provide scientific and theoretical basis for seed germination and strong seedling cultivation of K. everyniana.【Method】 A single factor complete randomized experiment design was employed to carry out the trial of seed soaking with different concentrations of melatonin solutions. Seed germination properties were recorded among treatments, and the changes of seedling growth, photosynthetic pigments and enzyme activities were measured. 【Result】 The germination rates, germination potentials, mean germination time, germination index, chlorophyll content, carotenoid content, activities of SOD, POD, CAT and MDA content were 40.4%-60.0%, 14.5%-32.1%, 12.6-14.6 day, 4.2-7.2 seed/day, 0.96-1.43 mg/g, 0.16-0.23 mg/g, 185.4-360.7 U/（g·min）, 24.4-64.9 U/（g·min）, 1 532.1-1 916.3 U/（g·min） and 9.5-25.6 nmol/g, respectively, with all parameters of the melatonin-treated seeds or seedlings significantly higher than those of control （P<0.05 or P<0.01）. 【Conclusion】 Soaking seeds with 0.3 mmol/L melatonin solution was found to be beneficial for seed germination, photosynthetic pigment formation, improving the activities of protective enzymes, and promoting the growth of seedlings, which could be applied to seed treatment of K. everyniana.

【Objective】 This study purposes to explore the effects of single endophytic fungus and mixed endophytic fungi on the growth and photosynthesis of Cunninghamia lanceolata seedlings. 【Method】 The endophytic fungi isolated from the root, stem and leaf samples of Chinese fir with robust growth and no diseases and insect pests were used as inoculated seedlings. The growth characteristics and photosynthetic physiological indexes of C. lanceolata seedlings were determined by inoculating the single or mixed growth promoting fungi. 【Result】 Four endophytic fungi Trichoderma spirale （S10）, Hypoxylon （S22）, Colletotrichum gloeosporioides （S24） and Umbelopsis dimorpha （S38） were successfully colonized in the root of C. lanceolata seedlings. Through the analysis of seedling height diameter, aboveground and underground biomass, root to shoot ratio and other growth characteristics, it can be seen that： single strain S24 and mixed strains S10×S22 and S22×S38 significantly promoted the growth of seedling height and ground diameter of C. lanceolata seedlings. Compared with CK group, the growth rates of single strain S24 on seedling height and ground diameter of C. lanceolata seedlings were 2.99% and 15.66%, respectively. The growth rates of seedling height and ground diameter for mixed strains were 13.81% and 3.04%, respectively, for S10×S22, and 18.99% and 11.01%, respectively, for S22×S38. The aboveground and underground biomass growth rates of individual strains S22, S24 and S38 were S22（22.86% and 58.57%）、S24（18.94% and 53.74%）and S38（15.87% and 57.40%）, respectively, compared with CK group. By analyzing chlorophyll content, chlorophyll fluorescence parameters （F₀、F_v/F_m） and other photosynthetic characteristics, it can be seen that： single strain S38 and mixed strains S10×S38 and S22×S24 significantly increased the chlorophyll content of seedlings at 45-60 d stage. Compared with CK group, chlorophyll content of C. lanceolata in S38 and CK group were 11.15%higher. The growth rates of mixed strains were as follows： S10×S38（2.25% and 17.53%）and S22×S24（10.72% and 13.51%）. C. lanceolata seedlings inoculated with single strain S24 and mixed strain S22×S38 had a lower F₀ value than that inoculated with other strains. The F_v/F_m value ranged from 0.80 to 0.85 across all treatments. 【Conclusion】 These results indicated that both single endophytic fungus and mixed endophytic fungus inoculations significantly improved the biomass and photosynthesis of C. lanceolata seedlings, and could effectively promote the growth of C. lanceolata seedlings. The growth promotion effect of mixed endophytic fungi was stronger than that of single endophytic fungus.

【Objective】 This study aims to reveal the difference of root morphology and carbon（C）, nitrogen（N）and phosphorus（P）stoichiometry characteristics of roots at different orders for tree at different growth stages. 【Method】 Taking the Cryptomeria japonica var. sinensis plantations of middle-aged forests（13 a）, mature forests（33 a）and over-mature forests （53 a）in Rain Area of Western China as the research objects, this study compared and analyzed the root morphology and root function modules of 1-5 grades of C. japonica var. sinensis plantations at different ages. 【Result】 ①With the increase of stand age, the average diameter of grade 1-5 fine roots increased first and then decreased, the specific root length decreased first and then increased, and the specific surface area decreased gradually, while the root tissue density showed no obvious change. With the increase of root order, the average fine root diameter of the same stand age increased, while the specific root length and specific surface area decreased gradually.②With the increase of stand age, the carbon content of C. japonica var. sinensis fine roots as a whole showed a trend of increasing first and then decreasing, the P content, C/N ratio, and C/P ratio showed a trend of decreasing first and then increasing, and the N/P ratio did not change significantly. Moreover, the N content of the absorptive root was higher than that of the transportive root, while the carbon content, C/N ratio, and C/P ratio of the transportive root were all higher than the absorptive root.③The carbon content of fine roots was significantly negatively correlated with phosphorus content, but was significantly positively correlated with the C/N ratio and C/P ratio. The N content was significantly positively correlated with P content, N/P ratio, specific root length and specific surface area, and was significantly negatively correlated with C/N ratio, C/P ratio, diameter and tissue density. 【Conclusion】 In summary, the fine root morphology and stoichiometry of C. japonica var. sinensis plantation have strong plasticity at different growth stages and root orders. The results of the study are helpful for in-depth understanding of the strategy of forest resource absorption and nutrient balance, and can provide a theoretical basis for the sustainable management and operation of plantations.

【Objective】 This study was aimed to explore the differential effects of immunocastration versus surgical castration on skeletal muscle accumulation as well as its underlying mechanisms using the male SD rat as model. 【Method】 The gastrocnemius muscle mass, histology as well as its accumulation-associated hormone and gene expression level were analyzed comparatively between immunocastrated and surgically castrated male rats. 【Result】 Compared to immunocastration, surgical castration caused lower serum IGF1 and higher corticosterone levels, higher gastrocnemius myostatin （Mstn） mRNA expression and higher serum urea nitrogen levels in male rats. 【Conclusion】 Lower IGF1 and higher glucocorticoid levels were the important reasons to cause more skeletal muscle myolysis in surgically castrated males than that in immunocastrated males.

【Objective】 Understanding the space utilization and activity regularity of animals during estrus is an important basis for carrying out animal field-introduction projects. 【Method】 In order to clarify the differences in these patterns between successful field-introduced giant pandas and failed field-introduced giant pandas during estrus in the field environment, and to find the reasons why field-rescued pandas can mate successfully while captive pandas failed, this paper investigated the movement date of two field-rescued individuals who have been kept in captivity for a long time and three captive individuals who haven't learnt any wild training with GPS collars in the Wolong National Nature Reserve. The GPS data were calculated in ArcGIS. 【Result】 The results showed that the minimum convex polygon home range of field-introduced pandas is from 7.19 km² to 75.21 km², and the MCP home range of unsuccessful field-introduced pandas was significantly larger than successful pandas. Meanwhile, the average daily movement distance of unsuccessful individuals was also larger than successful individuals [（493.11±57.45） m<（789.57±284.88） m]; the MCP home range before estrus, during estrus and after estrus were （1.94±1.11） km², （8.68±6.95） km² and （1.19±1.34） km².The MCP home range during estrus was significantly larger than that before and after estrus. Moreover, in different period, the giant pandas also have distinct different preferences for geographical factors. Meanwhile, the distance between scent trees and activity fixes during estrus of successful and failed field-introduced giant pandas is also has significant differences. The successful field-introduced giant pandas are inclined to be around the scent trees during estrus. 【Conclusion】 There are differences in the space utilization and activity patterns between successful and failed field-introduced giant pandas during different period. Compared with the successfully field-introduced pandas, the failed pandas had larger home range and average daily movement distance in the wild, and the activity fixes of successful pandas were more inclined to scent trees during estrus. It is suggested that in the future work, we should consider the wild training of giant pandas before the field introduction, focusing on the training of wild survival skills, improving the ability of communication and reproduction, so as to lay the foundation for the successful field-introduction of captive giant pandas in the wild.

【Objective】 The emergence of industrialized professional mushroom sticks has significantly improved the production efficiency of edible fungus growers, and played an important role in increasing farmers' income and high-quality production. In reality, the promotion of professional mushroom sticks is not ideal. Therefore, it has certain practical guiding significance to study the choice of farmers' edible fungi production behavior. 【Method】 Based on the survey data of farmers in 11 major edible mushroom producing provinces in China, this paper uses the probit model and the intermediary effect model to empirically test the effect of signing a production agreement and factory cognition on farmers' selection of mushroom production behavior. 【Result】 The results show that： First, signing a production agreement with edible fungus companies or cooperatives can significantly promote edible fungus growers to choose professionally produced fungus sticks. Second, the higher the farmers' cognition of factory-based professional stick making is, the easier it is for them to choose professionally produced fungus sticks. Third, farmers' cognition of factory-based professional stick making plays an intermediary role between signing a production agreement and the choice of farmers' production methods, that is, signing a production agreement can influence farmers' behavior in choosing professional bacterial sticks by improving farmers' cognition of factory-made professional sticks. 【Conclusion】 Based on this, it is concluded that the"farmers + cooperatives/companies"model should be actively promoted, the technical promotion capabilities of edible fungi enterprises and cooperatives should be improved, and farmers'cognition of the factory-based professional stick-making model should be promoted in various ways to promote the transformation and upgrading of farmers' production methods.

【Objective】 In order to explore the response mechanism of AP2/ERF transcription factor in mung bean under drought stress. 【Method】 The interspecies homology,interacting protein function and cis-acting elements of VrERF family genes in mung bean were analyzed based on the whole genome sequencing data. The tissue-specific expression of each VrERF gene and the difference of gene expression under drought stress were analyzed by transcriptome sequencing data and verified by qRT-PCR. 【Result】 The ERF family gene of mung bean had that same evolutionary origin as those of pea,kidney bean and soybean,and also had obvious gene expansion. The promoter region of VrERF gene has many cis-acting elements related to hormone response,stress response and growth. In addition,there are extensive interactions among different VrERF proteins in mung bean,which may participate in the defense response of mung bean to abiotic stress through interaction with bZIP,RAP2.4 and STZ. Gene expression analysis shows that the expression of most VrERF family genes has strong tissue specificity,and VrERF7/62 is significantly down-regulated in mung bean VC1973A and JP226873 leaves under drought stress. qRT-PCR analysis also showed that the expression of VrERF7/62 was significantly down-regulated by drought stress. 【Conclusion】 This study speculated that VrERF7/62 might play a negative role in the response to drought stress in mung bean,and laid a foundation for the study of the expression and gene function of AP2/ERF family genes in mung bean.

【Objective】 Under the background of coordinating regional development and realizing rural revitalization, the study of the spatial-temporal variation characteristics provides new research models for land spatial decision in Sichuan province. 【Method】 Based on the data of farmland consolidation projects in Sichuan province from 2016 to 2020, this paper studies the spatial-temporal variation characteristics and development trend of its quantity and scale of projects by using exploratory spatial pattern analysis method, gravity center model and standard elliptic difference analysis method. 【Result】 ①In terms of the spatial pattern of the projects quantity and scale, the watershed farmland consolidation projects is mainly based on regulation projects, showing a distribution pattern of more in the East and less in the West. Among them, the regulation projects are clustered in 5 watersheds such as the middle and lower reaches of Tuojiang River in northeast Sichuan and Chengdu plain economic zone in block forms. The development projects are mainly focused in southern SiChuan and panxi economic zone in the soil and water conservation and biodiversity conservation and restoration area of Jinsha River dry hot valley and the soil and water conservation and biodiversity conservation and restoration area of Jinping Mountain Yalong River downstream in southern Sichuan and northeast Sichuan economic zones by strip and group-like forms. The reclamation projects are mainly scattered in Chengdu plain and northwest Sichuan ecological demonstration economic zone in Qionglai Mountain Daxiangling water conservation and biodiversity conservation and restoration area. ②In terms of the annual project of the moving track and moving trend, the scale of annual regulation projects shows a moving track of “Southwest-Northeast”. The annual moving track of the reclamation project shows a moving trend of “Northwest-Northeast-Southwest”. The annual moving track of the development project generally shows a moving trend towards “northeast”. ③According to the above spatiotemporal differentiation characteristics, it is generally divided into two stages. elopment problems in each stage, corresponding suggestions are put forward from the aspects of matching, development weakness and development mode. Above study coordinates the spatial layout and spatial decision-making of land consolidation projects problems required in the Sichuan basin under the “mountain-basin” terrain. 【Conclusion】 Based on the above analysis, land consolidation in Sichuan Province should focus on optimizing the proportion of population, economy, resources and environment in local areas, invigorating idle land, supplementing the development weaknesses of major facilities and human settlements, improving the allocation capacity of spatial elements, especially land elements, and optimizing the spatial development mode, so as to improve the spatial organization efficiency of urbanization in the basin.

【Objective】 The objective of this study is to explore whether hydrogen sulfide can improve drought resistance of millet by mediating antioxidant system. 【Method】 We selected Jingu No.21 millet as the research material and used the method of soil culture to study the effects of four different treatments （water, drought, drought + hydrogen sulfide, drought + hydroxylamine） on the growth, morphology, and leaf damge of millet seedlings, contents of malondialdehyde and hydrogen peroxide in leaves, antioxidant content and antioxidant enzyme activity in leaves of millet seedlings. 【Result】 Under drought stress, 100 μmol/L sodium hydrosulfide significantly promoted the growth of millet seedlings and maintained their normal morphology; significantly increased the antioxidant content and antioxidant enzyme activity in seedlings, resulting in a significant decrease in the content of malondialdehyde and hydrogen peroxide in leaves, thereby reducing the degree of damage to leaves, and enhancing the drought resistance of millet. 【Conclusion】 The signaling molecule hydrogen sulfide can improve the drought resistance of millet by mediating the antioxidant system, which provides a reference for the in-depth study of the physiological function of hydrogen sulfide, and also provides a theoretical basis for the research and development of new plant drought resistance agents based on hydrogen sulfide signaling.