毛籽红山茶叶绿体基因组特征及其系统发育分析

doi:10.16036/j.issn.1000-2650.202202074

摘要/Abstract

摘要： 【目的】对毛籽红山茶（Camellia trichosperma）叶绿体全基因组进行序列特征分析并构建系统发育树,判断其分类学地位。【方法】基于高通量测序技术对毛籽红山茶叶绿体全基因组进行测序、组装、注释和序列特征分析及构建系统发育树。【结果】毛籽红山茶叶绿体全基因组长度为156 605 bp,具有典型的四分体结构,其IR区GC含量最高,为40.5%。通过注释预测到87个蛋白质编码基因,36个tRNA基因以及8个rRNA基因。简单重复序列（SSR）检测表明,叶绿体基因组包含68个SSR位点,单核苷酸、二核苷酸、三核苷酸、四核苷酸和六核苷酸数量分别为48、4、1、3和12。密码子偏好性分析发现亮氨酸密码子使用率最高,色氨酸使用率最低。【结论】毛籽红山茶与红山茶组（Sect. Camellia）的山茶（C. Japonica）和浙江红山茶（C. Chekiangoleosa）亲缘关系较近,但与同组的滇山茶（C. Reticulata）和南山茶（C. Semiserrata）亲缘关系较远。

关键词: 毛籽红山茶, 叶绿体基因组, 系统发育

Abstract: 【Objective】 This paper is to identify the taxonomic status of Camellia trichosperma by analyzing sequence characteristics of the chloroplast genome and constructing phylogenetic tree. 【Method】 The chloroplast genome of Camellia trichosperma was sequenced, assembled, annotated; sequence feature analyed and phylogenetic tree constructed by high-throughput sequencing technology. 【Result】 Camellia trichosperma chloroplast genome length was 156 605 bp, with typical tetrad structure, and its GC content in IR region was the highest （40.5%）. 87 protein-coding genes, 36 tRNA genes and 8 rRNA genes were predicted by annotation. Simple repeat sequence （SSR） analysis showed that the chloroplast genome contained 68 SSR loci, in which the number of mononucleotide, dinucleotide, trinucleotide, tetranucleotide and hexanucleotide were 48, 4, 1, 3 and 12, respectively. Codon bias analysis showed that leucine codon utilization rate was the highest and tryptophan was the lowest. 【Conclusion】 Phylogenetic analysis showed that Sect. Camellia was closely related to C. japonica and C. Chekiangoleosa. However, it was relatively distantly related to C. reticulata and C. semiserrata in the same group.

Key words: Camellia trichosperma, chloroplast genome, phylogeny

中图分类号:

S685.14

郑倩, 童一涵, 孔庆博, 冯士令, 周莉君, 丁春邦, 陈涛. 毛籽红山茶叶绿体基因组特征及其系统发育分析[J]. 四川农业大学学报, 2022, 40(4): 574-582.

ZHENG Qian, TONG Yihan, KONG Qingbo, FENG Shiling, ZHOU Lijun, DING Chunbang, CHEN Tao. Characterization of Complete Chloroplast Genome and Phylogenetic Analysis of Camellia trichosperma Chang[J]. Journal of Sichuan Agricultural University, 2022, 40(4): 574-582.

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