生防芽孢杆菌与杀菌剂的筛选复配及对栾树枝枯病防控效果

doi:10.16036/j.issn.1000-2650.2021.01.009

摘要/Abstract

摘要： 【目的】为提高生防芽孢杆菌防治栾树枝枯病效果,减少化学药剂使用量、缓解化学药剂抗药性问题,探讨生防芽孢杆菌与杀菌剂对栾树枝枯病的复配防效。【方法】采用平板对峙法筛选生防菌,生长速率法筛选杀菌剂,分光光度计测定杀菌剂与生防菌生物相容性,Wadley法确定复配比例。【结果】筛选出一株解淀粉芽孢杆菌对小新壳梭孢拮抗效果最强;50%多菌灵可湿性粉剂EC₅₀值,最低为0.123 mg/L,且与解淀粉芽孢杆菌具有生物相容性。50%多菌灵与解淀粉芽孢杆菌粗提物体积比为5 ∶ 1时,EC_50(ob)值为0.039 mg/L,SR值为3.783。复配剂对栾树枝枯病防效达83.83%。【结论】多菌灵与解淀粉芽孢杆菌联用防治栾树枝枯病,显著高于单剂防治效果,混剂施用可减少化学杀菌剂使用量。

关键词: 小新壳梭孢, 芽孢杆菌, 杀菌剂, 毒力测定, 协同防治, 栾树

Abstract: 【Objective】 The aim of this study was to improve the effect of biocontrol Bacillus on the control of branch withered disease on Koelreuteria paniculata,reduce the amount of chemical agents used,and alleviate the problem of chemical resistance,and to explore the compound control effect of biocontrol Bacillus and fungicide.【Method】 The biocontrol Bacillus was screened by plating method,the fungicide was screened by the growth rate method.The biocompatibility of biocontrol Bacillus and fungicide was determined by spectrophotometer,and the compound ratio was determined by Wadley method.【Result】 One Bacillus amyloliquefaciens strain was screened with the strongest antagonistic effect on Neofusicoccum parvum; the EC50 value of carbendazim 50% WP was the lowest,which was 0.123 mg/L,and it had biocompatibility with Bacillus amyloliquefaciens.When the volume ratio of carbendazim 50% to Bacillus amyloliquefaciens was 5 : 1,the EC50 (ob) value was 0.039 mg/L,and the SR value was 3.783.The control effect of the compound agent on branch withered disease of Koelreuteria paniculata was 83.83%.【Conclusion】 The control effect of carbendazim combined with Bacillus amyloliquefaciens on branch withered disease of K.paniculata was significantly higher than that of single agent.The application of compoud agent could reduce the use of chemical fungicides.

Key words: Neofusicoccum parvum, Bacillus, fungicide, virulence test, synergistic control, Koelreuteria paniculate

中图分类号:

S482.2

曾艳玲, 刘韩, 黎肇家, 李姝江. 生防芽孢杆菌与杀菌剂的筛选复配及对栾树枝枯病防控效果[J]. 四川农业大学学报, 2021, 39(1): 55-62.

ZENG Yanling, LIU Han, LI Zhaojia, LI Shujiang. Screening and Compounding of Biocontrol Bacillus and Fungicide and Their Control Effect on Branch Withered Disease on Koelreuteria paniculata[J]. Journal of Sichuan Agricultural University, 2021, 39(1): 55-62.

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