脱落酸长距离运输的生物学意义和分子机制解析

doi:10.16036/j.issn.1000-2650.2021.01.001

四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (1): 1-3.doi: 10.16036/j.issn.1000-2650.2021.01.001

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脱落酸长距离运输的生物学意义和分子机制解析

钦鹏, 张国华, 胡彬华, 李仕贵^*

四川农业大学水稻研究所,成都 611130

收稿日期:2021-01-27 出版日期:2021-02-28 发布日期:2021-03-05
作者简介:钦鹏,博士,教授,主要从事高温影响水稻灌浆结实的分子机制、优异等位基因挖掘和应用研究,E-mail:qinpeng@sicau.edu.cn;李仕贵,博士,教授,主要从事控制水稻主要农艺性状分子机制的解析、优异等位基因挖掘和应用研究,E-mail:lishigui@sicau.edu.cn。
基金资助:
国家自然科学基金项目(31771759,31771760); 科技部项目(2016ZX08001-003-005)

Revealing the Biological Significance and Molecular Mechanism of Abscisic Acid Long-Distance Transport in Plants

QIN Peng, ZHANG Guohua, HU Binhua, LI Shigui^*

Rice Research Institute,Sichuan Agricultural University,Chengdu 611130,China

Received:2021-01-27 Online:2021-02-28 Published:2021-03-05

摘要/Abstract

摘要： 【目的】水稻灌浆期高温严重影响其产量和品质,解析温度影响灌浆的分子机制,对保障水稻高产和优质意义重大。【方法】利用水稻中一份温度敏感的灌浆缺陷突变体dg1,结合遗传、生理、生化和³H-ABA feeding等技术方法,解析温度影响水稻灌浆的分子机制。【结果】我们克隆了一个调控水稻籽粒灌浆的关键基因DG1。DG1编码一个MATE转运蛋白,该蛋白具有外排脱落酸(abscisic acid,ABS)活性,在节和小花轴参与调控叶片到颖果的ABA长距离运输。高温通过诱导DG1高表达促进叶片到颖果的ABA长距离转运,该长距离转运的ABA诱导淀粉合成关键基因表达,从而确保高温下水稻籽粒正常灌浆。相关研究成果以“Leaf-derived ABA regulates rice seed development via a transporter-mediated and temperature-sensitive mechanism”为题于2021年1月发表在国际期刊Science Advances(10.1126/sciadv.abc8873)。【结论】该成果揭示了DG1通过调控叶片到颖果ABA长距离运输确保高温下种子正常发育的分子机制。

关键词: 水稻, MATE转运蛋白, 脱落酸, 长距离运输, 温度响应

Abstract: 【Objective】 Rice is easy to encounter to high temperature during grain filling period in summer,that seriously affects rice yield and quality.It is of great significance for ensuring high yield and good quality of rice to reveal the molecular mechanism of rice grain filling affected by temperature.【Method】 We take advantage of a rice temperature-sensitively defective grain-filling mutant,named dg1 (defective grain-filling 1) coupling with genetic,physiological,biochemical and ³H-ABA feeding methods,to analyze the molecular mechanism of rice grain filling affected by temperature.【Result】 We clone a key gene which regulates rice grain filling.DG1 encodes a MATE transporter which has abscisic acid(ABS) efflux activity,and regulates the leaf-to-caryopsis ABA long distance transport at node and rachilla.Higher temperature promotes the leaf-to-caryopsis ABA transport efficiency by inducing the expression level of DG1.Such long-distance transported ABA ensure normal grain filling at high temperature by inducing the expression of several key genes involved in starch synthesis.【Conclusion】 Published on Science Advances in January 2021 with the title "Leaf-derived ABA regulates rice seed development via a transporter-mediated and temperature-sensitive mechanism" (10.1126/sciadv.abc8873) This result reveals the molecular mechanism that DG1 ensures normal grain filling at higher temperature by regulating leaf-to-caryopsis ABA long-distance transport.

Key words: rice, MATE transporter, abscisic acid, grain filling, temperature response

中图分类号:

S511

钦鹏, 张国华, 胡彬华, 李仕贵. 脱落酸长距离运输的生物学意义和分子机制解析[J]. 四川农业大学学报, 2021, 39(1): 1-3.

QIN Peng, ZHANG Guohua, HU Binhua, LI Shigui. Revealing the Biological Significance and Molecular Mechanism of Abscisic Acid Long-Distance Transport in Plants[J]. Journal of Sichuan Agricultural University, 2021, 39(1): 1-3.

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脱落酸长距离运输的生物学意义和分子机制解析

Revealing the Biological Significance and Molecular Mechanism of Abscisic Acid Long-Distance Transport in Plants

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