四川省套作玉米茎腐病致病镰孢菌的分离与鉴定

doi:10.16036/j.issn.1000-2650.2018.05.005

四川农业大学学报 ›› 2018, Vol. 36 ›› Issue (05): 598-604.doi: 10.16036/j.issn.1000-2650.2018.05.005

四川省套作玉米茎腐病致病镰孢菌的分离与鉴定

周欢欢¹, 严雳¹, 王对平¹, 雍太文^{1, 2}, 龚国淑¹, 尚静^{1, 2}, 杨文钰^{1, 2}, 常小丽^{1, 2, *}

1. 四川农业大学农学院,成都 611130;
2. 四川省作物带状复合种植工程技术研究中心,成都 611130

收稿日期:2018-01-20 出版日期:2018-10-28 发布日期:2021-03-01
通讯作者: 常小丽, 博士, 副教授, 主要从事真菌病害发生及作物抗病机制研究, E-mail：xl_changkit@126.com。
作者简介:周欢欢,硕士。
基金资助:
四川省科技厅项目(2015NZ0040)。

Identification of Fusarium Species Causing Maize Stalk Rot in Maize Soybean Relay Intercropping Pattern in Sichuan Province

ZHOU Huanhuan¹, YAN Li¹, WANG Duiping¹, YONG Taiwen^{1, 2}, GONG Guoshu¹, SHANG Jing^{1, 2}, YANG Wenyu^{1, 2}, CHANG Xiaoli^{1, 2, *}

1. Department of Agriculture,Sichuan Agriculutral University,Chengdu 611130,China;
2. Sichuan Engineering Research Center for Crop Strip Intercropping System,Chengdu 611130,China

Received:2018-01-20 Online:2018-10-28 Published:2021-03-01

摘要/Abstract

摘要： 【目的】明确四川省套作玉米茎腐病镰孢菌的种类。【方法】2016——2017年采集仁寿、崇州、雅安、荣县4个县市套作玉米茎腐病样品进行分离与纯化,采用形态特征及基于rDNA-ITS和EF1-α序列分析鉴定镰孢菌种类,以幼苗刺伤接种法检测致病性。【结果】共获得103株纯培养物,其中71株为镰孢菌,包括层生镰孢菌、腐皮镰孢菌、轮枝镰孢菌、禾谷镰孢菌、尖孢镰孢菌、藤仓镰孢菌和木贼镰孢菌,分离频率依次为35.21%、16.9%、15.49%、11.27%、8.45%、7.04%和5.63%,且各地区镰孢菌种类及分离频率存在差异;致病性测定表明,禾谷镰孢菌致病性最强,层生镰孢菌和轮枝镰孢菌次之,尖孢镰孢菌和木贼镰孢菌致病性较弱,藤仓镰孢菌和腐皮镰孢菌致病性最弱。【结论】四川套作玉米茎腐病优势致病菌为层生镰孢菌、轮枝镰孢菌和禾谷镰孢菌。

关键词: 玉米大豆带状套作, 玉米茎腐病, 镰孢菌, 分子鉴定, 致病性检测

Abstract: 【Objective】 This study aims to find out the population of Fusarium species causing maize stalk rot in maize soybean relay intercropping pattern in Sichuan Province. 【Method】 The samples were collected from the infected stem intercropping maizes in four cities including Renshou,Chongzhou,Ya'an,and Zigong in Sichuan province during 2016——2017. Fusarium species were obtained through tissue isolation and purification,and identified based on morphological characters and molecular iden-tification based on PCR amplification of the gene fragements of rDNA ITS and translation elongation factor 1α. Pathogenicity test was performed on maize seedlings through wounding inoculation. 【Result】We found that total 71 strains of Fusarium species were obtained from 103 isolates from diseased maize,and were identified into seven species including Fusarium proliferatum, F. solani,F. verticillioides,F. graminearum,F. oxysporum, F. fujikuroi and F. equiseti with the isolation frequencies for 35.21%、16.9%、15.49%、11.27%、8.45%、7.04% and 5.63%. Pathogencity tests with representative strains of each Fusarium species showed that F. graminearum had the strongest pathogenicity on maize seedling,F. proliferatum,F. fujikuroi and F. verticillioides showed weaker pathogenicity, when F. oxysporum, F. solani and F. equiseti was the weakest pathogenicity. 【Conclusion】 This study indicates that maize stalk rot is predominantly caused by F. proliferatum,F. verticillioides,and F. graminearum in maize soybean relay intercropping in Sichuan province.

Key words: maize soybean relay intercropping, maize stalk rot, Fusarium species, pathogen identification, pathogenicity test

中图分类号:

S432.1

周欢欢, 严雳, 王对平, 雍太文, 龚国淑, 尚静, 杨文钰, 常小丽. 四川省套作玉米茎腐病致病镰孢菌的分离与鉴定[J]. 四川农业大学学报, 2018, 36(05): 598-604.

ZHOU Huanhuan, YAN Li, WANG Duiping, YONG Taiwen, GONG Guoshu, SHANG Jing, YANG Wenyu, CHANG Xiaoli. Identification of Fusarium Species Causing Maize Stalk Rot in Maize Soybean Relay Intercropping Pattern in Sichuan Province[J]. Journal of Sichuan Agricultural University, 2018, 36(05): 598-604.

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四川省套作玉米茎腐病致病镰孢菌的分离与鉴定

Identification of Fusarium Species Causing Maize Stalk Rot in Maize Soybean Relay Intercropping Pattern in Sichuan Province

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