四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (05): 558-563.doi: 10.16036/j.issn.1000-2650.2020.05.008

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辽细辛菌核病拮抗菌的鉴定及生物学特性研究

李玲, 孙文松*, 张天静, 沈宝宇, 刘莹   

  1. 辽宁省经济作物研究所,辽宁 辽阳 111000
  • 收稿日期:2020-04-11 出版日期:2020-10-28 发布日期:2021-02-05
  • 作者简介:李玲,研究员。*责任作者:孙文松,研究员,主要从事中药材绿色标准化种植技术研究,E-mail:sunwensong12@126.com。
  • 基金资助:
    国家中药材产业技术体系项目(CARS-21-06); 辽宁省科学事业公益性研究基金(20180048); 辽宁省农业科学院重大科研成果培育项目

Identification and Biological Characteristics of Antagonistic Fungal Strains Against Sclerotinia asari

LI Ling, SUN Wensong*, ZHANG Tianjing, SHEN Baoyu, LIU Ying   

  1. Liaoning Institute of Industrial Crops,Liaoyang 111000,Liaoning,China
  • Received:2020-04-11 Online:2020-10-28 Published:2021-02-05

摘要: 【目的】发掘高效的辽细辛菌核病拮抗菌生物防治资源菌。【方法】以收集的对植物病菌有拮抗作用的31个菌株作为候选拮抗菌株,以细辛核盘菌(Sclerotinia asari)为靶标菌,采用平板对峙法,筛选具有拮抗作用的菌株,结合形态学观察和ITS序列分析确定拮抗菌株分类地位,同时对其生物学特性进行了研究。【结果】筛选出一株对辽细辛核盘菌有强拮抗作用的菌株(AR-4-1),经rDNA-ITS基因序列分析和形态学鉴定,该拮抗菌株为伊比利亚单孢囊菌(Monosporascus ibericus)。AR-4-1菌株最适生长温度为25 ℃,致死温度为60 ℃,适宜酸碱度范围为pH值5~9,最适培养基为改良马丁氏(MA)和马铃薯葡萄糖(PDA),最适氮源为硫酸铵和硝酸钾,最适碳源为葡萄糖和蔗糖。【结论】获得1株能高效拮抗辽细辛核盘菌菌株伊比利亚单孢囊菌AR-4-1,伊比利亚单孢囊菌作为拮抗菌株防治辽细辛病害在国内首次报道。为辽细辛菌核病生物防治制剂研发提供了基础材料。

关键词: 辽细辛菌核病, 生物防治, 拮抗作用, 生物学特性, 伊比利亚单孢囊菌

Abstract: 【Objective】 To screen and identify effective antifungal strains for biocontrol to the Sclerotinia rot of asarum. 【Method】 The strain with strong antagonistic effect against Sclerotinia asari was screened from 31 strains with antagonistic effect on plant pathogens by plate confrontation method.The strain was identified by its ITS-rDNA gene sequence analysis and morphological identification. At the same time, the biological characteristics of the strain were systematically studied. 【Result】 A strain (AR-4-1) with strong antagonistic effect against Sclerotinia asari was screened. The strain was identified as M. ibericus Collado, Ant. González, Stchigel, Guarro & Peláez). the optimal temperature for AR-4-1 growth was 25 ℃ and the lethal temperature was 60 ℃;the optimum pH value was pH 5-9. The suitable medium for AR-4 growth was MA and PDA, optimal nitrogen source was ammonium sulfate and potassium nitrate, and optimal carbon sources are glucose and sucrose. 【Conclusion】 It provides base material for research and development of biological control agents of Sclerotinia rot of asarum.

Key words: Sclerotinia of asarum, biological control, antagonism effect, biological characteristics

中图分类号: 

  • S435.675