超长链脂肪酸调控稻瘟病菌致病的分子机制及作为防治靶标的用途

doi:10.16036/j.issn.1000-2650.2020.05.001

四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (05): 509-512.doi: 10.16036/j.issn.1000-2650.2020.05.001

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超长链脂肪酸调控稻瘟病菌致病的分子机制及作为防治靶标的用途

贺闽, 许有嫔, 戚拓, 宿加, 陈金华, 雷鸣亮, 陈学伟^*

四川农业大学西南作物基因资源发掘与利用国家重点实验室（筹）/水稻研究所,成都 611130

收稿日期:2020-10-17 出版日期:2020-10-28 发布日期:2021-02-05
作者简介:贺闽,副教授。^*责任作者：陈学伟,教授,主要从事水稻稻瘟病广谱抗性机制与育种应用研究,E-mail：xwchen88@163.com。
基金资助:
国家自然科学基金项目（31871920,31825022）; 四川省科技厅国际合作项目（2020YFH0137）

Molecular Regulation of Very-Long-Chain Fatty Acids on Pathogenicity of Magnaporthe oryzae and Its Utilization for Development of Fungicides

HE Min, XU Youpin, QI Tuo, SU Jia, CHEN Jinhua, LEI Mingliang, CHEN Xuewei^*

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Rice Research Institute, Sichuan Agricultural University,Chengdu,611130 China

Received:2020-10-17 Online:2020-10-28 Published:2021-02-05

摘要/Abstract

摘要： 【目的】有着“水稻癌症”之称的稻瘟病严重危害水稻生产,鉴定稻瘟病菌致病关键因子并以此作为靶标开发有效农药,对防控该病害具有重大意义。【方法】目前,虽已报道隔蛋白septin调控稻瘟病菌致病性,但其分子作用机理并不清楚,也尚未开发以septin功能为靶标的农药,于是我们利用遗传、生化、细胞生物和植物病理学等技术手段,对稻瘟病菌septin组装调控机制开展了分析。【结果】我们发现超长链脂肪酸（VLCFAs）对septin介导的致病起关键作用,并发现靶向抑制VLCFAs合成的metazachlor和diallate等药物对稻瘟病、玉米小斑病、小麦白粉病等真菌病害具有广谱防治效果,相关研究成果以“Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi”为题于2020年9月发表在国际期刊Nature Microbiology （doi.org/10.1038/s41564-020-00790-y）。【结论】该成果揭示了septin介导真菌致病的新机制,并为防治动植物真菌病害提供了新方案和新药物。

关键词: 稻瘟病菌, 杀真菌剂, 致病性, 隔蛋白, 超长链脂肪酸

Abstract: 【Objective】 Known as rice cancer, the blast disease severely threatens rice production. To ensure food security, it is important to identify key regulators for pathogenicity of blast fungus Magnaporthe oryzea and accordingly develop effective fungicides targeting the regulators for controlling this disease. 【Method】 Septin is crucial for host infection by M. oryzea, but its molecular mechanism is largely unknown. Fungicides targeting septin function have also not been developed. Then we adopt various techniques in genetics, biochemistry, cell biology and plant pathology to dissect the molecular regulation of septin organization in M. oryzae. 【Result】 We report that very-long-chain fatty acids (VLCFAs) play key roles in septin-mediated pathogenicity of M. oryzea. Besides, we confirm that chemicalsinhibiting VLCFAs biosynthesis, such as metazachlor and diallate, show broad-spectrum fungicidaleffects on rice blast,sourthern-corn leaf blight and wheat powdery mildew diseases. 【Conclusion】 Published on Nature Micro-biology in September 2020 with the title “Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi” (doi.org/10.1038/s41564-020-00790-y）,our findings reveal a novel mechanism on septin-mediated fungal pathogenicity and provide new control measure and effective chemicals for fungal diseases.

Key words: Magnaporthe oryzea, fungicides, pathogenicity, septin, very-long-chain fatty acids

中图分类号:

S435.111.4+1

贺闽, 许有嫔, 戚拓, 宿加, 陈金华, 雷鸣亮, 陈学伟. 超长链脂肪酸调控稻瘟病菌致病的分子机制及作为防治靶标的用途[J]. 四川农业大学学报, 2020, 38(05): 509-512.

HE Min, XU Youpin, QI Tuo, SU Jia, CHEN Jinhua, LEI Mingliang, CHEN Xuewei. Molecular Regulation of Very-Long-Chain Fatty Acids on Pathogenicity of Magnaporthe oryzae and Its Utilization for Development of Fungicides[J]. Journal of Sichuan Agricultural University, 2020, 38(05): 509-512.

参考文献

[1] DENG Y W, ZHAI K R, XIE Z, et al.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J]. Science, 2017, 355(6328): 962-965.
[2] ZHONG Z H, NORVIENYEKU J, CHEN M L, et al.Directional selection from host plants is a major force driving host specificity in Magnaporthe Species[J]. Scientific Reports, 2016, 6: 25591.
[3] DAGDAS Y F, YOSHINO K, DAGDAS G, et al.Septin-mediated plant cell invasion by the rice blast fungus, Magnaporthe oryzae[J]. Science, 2012, 336(6088): 1590-1595.
[4] BRIDGES A A, GLADFELTER A S.Fungal pathogens are platforms for discovering novel and conserved septin properties[J]. Current Opinion in Microbiology, 2014, 20: 42-48.
[5] OH Y, BI E F.Septin structure and function in yeast and beyond[J]. Trends in Cell Biology, 2011, 21(3): 141-148.
[6] DIMITRIOS A, SPILIOTIS E T.Septin mutations in human cancers[J]. Frontiers in Cell & Developmental Biology, 2016, 4: 122.
[7] BERTIN A, MCMURRAY M A, THAI L, et al.Phosphatidylinositol-4,5-bisphosphate promotes budding yeast septin filamentassembly and organization[J]. Journal of Molecular Biology, 2010,404(4): 711-731.
[8] SPILIOTIS E T, GLADFELTER A S.Spatial guidance of cell asymmetry: septin GTPases show the way[J]. Traffic, 2012, 13(2):195-203.
[9] BACH L, FAURE J D.Role of very-long-chain fatty acids in plant development, when chain length does matter[J]. Comptes Rendus Biologies, 2010, 333(4): 361-370.
[10] SHANG B S, XU C Y, ZHANG X X, et al.Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(18): 5101-5106.
[11] REZANKA T, KOLOUCHOVÁ I, GHARWALOVÁ L, et al.Identification and characterization of phospholipids with very long chain fatty acids in brewer's yeast[J]. Lipids, 2017, 52(12): 1007-1017.
[12] PAZ RAMOS A, LAGÜE P, LAMOUREUX G, et al. Effect of saturated very long-chain fatty acids on the organization of lipid membranes: a study combining 2H NMR spectroscopy and molecular dynamics simulations[J]. Journal of Physical Chemistry B, 2016,120(28): 6951-6960.
[13] OBARA K, KOJIMA R,KIHARA A, et al.Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[J]. Journal of lipid research, 2013, 54(3):831-842.
[14] KOYUNCU E, PURDY J G, RABINOWITZ J D, et al.Saturated very long chain fatty acids are required for the production of infectious human cytomegalovirus progeny[J]. PLoS Pathogens, 2013, 9(5): e1003333.
[15] RAMAKRISHNAN S, DOCAMPO M D, MACRAE J I, et al.The intracellular parasite Toxoplasma gondii depends on the synthesis of long-chain and very long-chain unsaturated fatty acids not supplied by the host cell[J]. Molecular Microbiology, 2015, 97(1): 64-76.
[16] HE M, SU J, XU Y P, et al.Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi[J]. Nature Microbiology, 2020. doi.org/10.1038/s41564-020-00790-y.

超长链脂肪酸调控稻瘟病菌致病的分子机制及作为防治靶标的用途

Molecular Regulation of Very-Long-Chain Fatty Acids on Pathogenicity of Magnaporthe oryzae and Its Utilization for Development of Fungicides

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