关键词: 根肿菌, 生理小种, 分子鉴定

Abstract: 【Objective】 In recent years, the clubroot has become a major disease of Brassica napus and other cruciferous crops in China. Plasmodiophora brassicae, as an obligate parasitic pathogen, is difficult for the single spore isolation and culture technology, which restricts the molecular identification of pathotype. The detection system established in the study is simple, specific and widely applicable, which can provide reference for the monitoring, early warning and the comprehensive prevention and control of pathotype 4 in feild. 【Method】 Transcriptome sequencing was used to screen the differentially expressed genes of P. brassicae pathotyoe 4 from other pathotypes infecting Brassica rapa. These genes were verified by conventional PCR.【Result】The two molecular marker genes, PBRA_000030 and Novel00510 were found in pathotype 4, which different from other test pathotypes 2,5,7,9,10 and 11. The dominant physiological pathotype 4 in clubtoot could be identified by using PCR with about 25 amplification cycles.【Conclusion】A simple, specific and widely applicable method was established,basing on the molecular marker genes obtained from the dominant pathotype 4 in China.

Key words: Plasmodiophora brassicae, pathotype, molecular identification