川西高寒土壤DOM荧光特征研究

doi:10.16036/j.issn.1000-2650.2020.03.005

摘要/Abstract

摘要： 【目的】研究高寒土壤溶解性有机质（DOM）含量和光谱学特征,为川西高原区域水陆碳循环以及高寒土壤资源管理等研究提供科学依据。【方法】研究了川西3种高寒生态系统（亚高山森林、高寒泥炭湿地和高寒草甸）中0~10 cm的表层土壤中的DOM,利用三维荧光分析仪测定了DOM荧光特征,采用平行因子法（EEM-PARAFAC）和荧光区域积分（FRI）等对DOM光谱学特征进行了研究。【结果】亚高山森林土壤中DOC、DON、TOC和TN含量明显高于高寒泥炭土和高寒草甸;亚高山森林土壤DOM芳香性与腐殖化程度最低,DOM稳定性最低,微生物可利用性最高;平行因子分析显示所有土壤DOM呈4个荧光组分,以蛋白类组分载荷最高,富里酸类组分载荷最低;亚高山森林土壤DOM中芳香蛋白类有机质占比最高,高寒泥炭湿地和高寒草甸土壤DOM中则是富里酸类有机质比例最高。【结论】3种高寒土壤的DOM及荧光组分差异显著,亚高山森林土壤DOM不稳定性更高。

关键词: 高寒土壤, 溶解性有机质, 光谱特性, 平行因子分析（PARAFAC）, 三维荧光积分（FRI）

Abstract: 【Objective】 To explore dissolved organic matter (DOM) concentration and spectral characteristics of alpine soils, which provide scientific basis for the study of water land carbon cycle and alpine soil resource management in western Sichuan Plateau. 【Method】 DOM in 0-10cm surface soil of three alpine ecosystems (subalpine forest, alpine peat wetland and alpine meadow) in Western Sichuan was extracted, and their fluorescence characteristics of DOM were measured by three-dimensional fluorescence analyzer. The data of fluorescence characteristics of DOM were analyzed by excitation emission matrix fluorescence and parallel factor analysis (EEM-PARAFAC) and fluorescence region integral (FRI). 【Result】 The concentrations of dissolved organic carbon, dissolved nitrogen, total carbon and total nitrogen in soil of subalpine forest were higher than those in alpine peat soil and alpine meadow soil significantly. Moreover, the DOM in subalpine forest soil showed the lowest degree of aroma and humification, the lowest stability, and the highest availability for microorganism. The results of EEM-PARAFAC analysis showed that there were four fluorescent components with the highest protein component and the lowest fulvic acid component in DOM of all of the three alpine soils. Additionally, the highest proportion of aromatic protein component was in DOM of subalpine forest soil, while the highest fulvic acids component was in DOM of alpine peat wetland soil. 【Conclusion】 It indicates significant differences between DOMs and their fluorescence characteristics in the three alpine soils, and the highest unstability of the DOM in soil of the subalpine forest.

Key words: alpine soil, dissolved organic matter, spectroscopic characteristics, parallel factor analysis, fluorescence regional integration

中图分类号:

X144

王姝, 秦纪洪, 谢冰心, 孙辉, 刘琛. 川西高寒土壤DOM荧光特征研究[J]. 四川农业大学学报, 2020, 38(03): 280-289.

WANG Shu, QIN Jihong, XIE Bingxin, SUN Hui, LIU Chen. Dissolved Organic Matter Fluorescence Characteristics of Alpine Soils in the Western Sichuan Plateau[J]. Journal of Sichuan Agricultural University, 2020, 38(03): 280-289.

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