油麦吊云杉天然种群针叶与种实表型性状变异分析

doi:10.16036/j.issn.1000-2650.2020.04.007

四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (04): 423-429.doi: 10.16036/j.issn.1000-2650.2020.04.007

油麦吊云杉天然种群针叶与种实表型性状变异分析

马月琴^1,2, 贾子瑞³, 莫劲雁⁴, 辜云杰¹, 李晓清^1,*

1.四川省林业科学研究院,成都 610081;
2.森林与草原防灾减灾工程研究中心,四川绵阳 621000;
3.中国林业科学研究院林业研究所,北京 100091;
4.九寨沟县林业和草原局,四川九寨沟 623400

收稿日期:2020-04-15 出版日期:2020-08-28 发布日期:2021-02-02
作者简介:马月琴,硕士研究生。^*责任作者：李晓清,博士,研究员,主要研究方向为森林培育,E-mail：464394961@qq.com。
基金资助:
国家自然基金青年项目（31500540）; “麦吊云杉种质资源遗传多样性保护示范”项目（川林科司工设201827号）

Analysis on the Phenotypic Variation of Needles and Seeds of Picea brachytyla Natural Population

MA Yueqin^1,2, JIA Zirui³, MO Jinyan⁴, GU Yunjie¹, LI Xiaoqing¹

1. Sichuan Academy of Forestry,Chengdu 610081,China;
2. Engineering Research Center for Forest and Grassland Disaster Prevention and Reduction,Mianyang normaluniversity,Mianyang 621000,Sichuan,China;
3. Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;
4. Jiuzhaigou County Forestry and Grassland Bureau,Jiuzhaigou County 623400,Sichuan,China

Received:2020-04-15 Online:2020-08-28 Published:2021-02-02

摘要/Abstract

摘要： 【目的】系统地揭示油麦吊云杉天然种群种实表型性状间的变异差异性,为油麦吊云杉的种质资源收集、遗传保育和合理利用提供科学依据。【方法】采用方差分析、多重比较、相关性分析、聚类分析等统计方法,对4个具有代表性的天然种群13个种实表型性状进行比较分析,研究种群间和种群内表型多样性以及表型性状间变异的相关性。【结果】①通过方差分析与多重比较表明,在种群间,种实性状的平均变异数从大到小依次为：神木垒（20.02%）>正干沟（19.20%）>油房沟（19.16%）>大川（13.70%）,神木垒种群变异程度最大,正干沟和油房沟种群间差异性较小,大川种群变异程度最小。种群内,表型性状的平均变异系数从大到小依次为：针叶（19.50%）>球果（15.86%）>种鳞（15.26%）>种翅（13.60%）,针叶性状变异最明显,球果和种鳞的变异较小,种翅性状变异程度最小。②从相关性分析中表明,种实表型性状种鳞长、种翅宽、球果宽三者间存在显著正相关关系,在种群内变异起重要作用;其中,种群内多样性变化程度高于种群间,是主要变异。③根据聚类分析,4个种群的13个表型性状可分为3类,第一类为油房沟与神木垒,种群的球果质量较大,针叶饱满厚实,均值较高,种鳞、种翅呈短窄型;第二类为大川,球果呈狭长扁短型,种鳞、种翅较大、针叶呈短窄型;第三类为正干沟,种鳞、种翅变化不稳定,球果质量最小,且分布海拔为最高。【结论】油麦吊云杉种实表型性状在种群间和种群内都存在明显的差异性变化。

关键词: 油麦吊云杉, 天然种群, 种实性状, 表型多样性

Abstract: 【Objective】 To reveal the genetic variation patterns of phenotypic traits in natural populations of Picea brachytyla. It provides scientific basis for germplasm collection, genetic conservation and rational utilization of Picea brachytyla. 【Method】 According to Variance Analysis, Multiple Comparison,Correlation Analysis and Cluster Analysis to analyze 13 phenotypic traits of 4 representative natural populations, to study correlations between phenotypic diversity and phenotypic variation among populations. 【Result】 ①The results of Variance Analysis and Multiple Comparison showed that the average variation of seed characters from big to small： SML（20.02%）>ZGG（19.20%）>YFG（19.16%）>DC（13.70%）. SML had the greatest degree of variation, the difference between ZGG and YFG had stable difference, DC had the least. Within the population, the average variation of phenotypic traits was in the order： conifer（19.50%）, cones（15.86%）, seed scales（15.26%）, and species wings（13.60%）. The variation of needles traits was obvious, cones and seed scales were stable, but the variation of species wings was not significant. ②From Correlation Analysis, there is a significant positive correlation between phenotypic traits, seed length, wing width and cone breadth, and seed scales, seed wings and cones play an important role in the variation within populations. Variation within populations is the main variation, and the degree of diversity is higher than that among populations.③According to Cluster Analysis, 13 phenotypic characters of the four populations can be divided into three categories. The first category is YFG and SML. The cone mass of the population is large, the needles are full and thick, the mean value is high, and the length and width of the seed scales and wings are short and narrow; The second category is DC, cone is narrow and flat, seed scales and wings are relatively large, and the needles are short and narrow; The third category is ZGG, the scales and wings were relatively high, and the cones were of the lowest mass and the highest elevation was distributed. 【Conclusion】 The phenotypic traits of Picea brachytyla var. Picea were different among populations.

Key words: Picea brachytyla, natural population, seed traits, phenotypic diversity

中图分类号:

Q948

马月琴, 贾子瑞, 莫劲雁, 辜云杰, 李晓清. 油麦吊云杉天然种群针叶与种实表型性状变异分析[J]. 四川农业大学学报, 2020, 38(04): 423-429.

MA Yueqin, JIA Zirui, MO Jinyan, GU Yunjie, LI Xiaoqing. Analysis on the Phenotypic Variation of Needles and Seeds of Picea brachytyla Natural Population[J]. Journal of Sichuan Agricultural University, 2020, 38(04): 423-429.

参考文献

[1] 李龙. 麦吊云杉及其相关物种的群体遗传学研究[C]. //中国植物学会系统与进化植物学专业委员会、云南省植物学会. 2011年全国系统与进化植物学暨第十届青年学术研讨会论文集.中国植物学会系统与进化植物学专业委员会、云南省植物学会, 2011: 87.
[2] 刘建泉, 宋秉明, 郝玉福. 祁连山青海云杉林抚育更新研究[J]. 江西农业大学学报, 1998, 20(1): 3-5.
[3] 唐巍, 徐润青. 油麦吊云杉人工林生长量分析[J]. 四川林勘设计, 1997(2): 21-26.
[4] 吴兆录, 党承林, 和兆荣, 等. 滇西北油麦吊云杉林生物量的初步研究[J]. 云南大学学报(自然科学版), 1994, 16(3):230-234.
[5] 吴兆录, 党承林, 和兆荣, 等. 滇西北油麦吊云杉林净第一性生产力的初步研究[J]. 云南大学学报(自然科学版), 1994, 16(3): 240-244.
[6] 陆缤. 油麦吊云杉繁育技术研究初探[J]. 内蒙古林业调查设计, 2016, 39(6): 61-64.
[7] 胡启鹏, 郭志华, 李春燕, 等. 植物表型可塑性对非生物环境因子的响应研究进展[J]. 林业科学, 2008, 44(5): 135-142.
[8] DONOHUE K, PYLE E H, MESSIQU A D, et al.Adaptive divergence in plasticity in natural populations of Inmpatiens capensis and its consequences for performance in novel habitats[J]. Evolution, 2001, 55(4): 692-702.
[9] ETTERSON J R.Evolutionary potential of Chamaecrista fascic-ulata in relation to climate change.I.Clinal patterns of selection along an enviromental gradient in the Great Plains[J]. Evolution, 2001, 58(7): 1446-1458.
[10] RICHARDS C L, PENNINGS S C, DONOVAN L A.Habitat range and phenotypic variation in salt marsh plants[J]. Plant Ecology, 2005, 176(2): 263-273.
[11] GHALAMBOR C K, MCKAY J K, CARROLL S P, et al. Adaptive versus non-adaptive phenotypic plasticity and the potential for contemporary adaptation in new enviroments[J].Function Ecology, 2007, 21(3): 394-407.
[12] 张翠琴, 姬志峰, 林丽丽, 等. 五角枫种群表型多样性[J]. 生态学报, 2015, 35(16): 5343-5352.
[13] 罗建勋, 顾万春. 云杉表型与同工酶遗传多样性研究进展[J]. 林业科学研究, 2004, 17(2): 255-262.
[14] 李文英, 顾万春. 栎类植物遗传多样性研究[J]. 世界林业研究, 2002, 15(2): 42-19.
[15] 罗建勋, 李晓清, 孙鹏, 等. 云杉天然种群表型变异研究[J].东北林业大学学报, 2003, 31(1): 9-11.
[16] FALKENHAGEN E R.Multivariate classification in provenance research[J]. Silvae Genetica, 1978, 27(1): 14-23.
[17] PUTENIKHIN V P.Phenotype analysis of Picea obovata in the southern Urals populations structure[J]. Lesovefenie, 1997(6):37-49.
[18] LI PENG, BEAULIEU J, BOUSQUET J.Genetic structure and patterns of genetic variation among populations in eastern White Spruce(Picea glauca)[J]. Canadian Journal of Forest Research,1997, 27(2): 189-198.
[19] 葛颂, 王明庥, 陈岳武. 用同工酶研究马尾松种群的遗传结构[J]. 林业科学, 1988, 24(4): 399-409.
[20] 李斌, 顾万春, 卢宝明. 白皮松天然种群种实性状表型多样性研究[J]. 生物多样性, 2002, 10(2): 181-188.
[21] 罗建勋, 顾万春. 云杉天然种群表型多样性研究[J]. 林业科学, 2005, 41(2):66-73.
[22] 罗建勋, 顾万春. 云杉天然种群种实性状变异研究[J]. 西北农林科技大学学报(自然科学版), 2004, 32(8): 60-66.
[23] 张含国, 孙立夫, 韩继凤, 等. 红皮云杉种群遗传多样的研究[J]. 植物研究, 2003, 23(2): 224-229.
[24] 罗建勋. 云杉天然种群遗传多样性研究[D]. 北京: 中国林科院, 2004.

油麦吊云杉天然种群针叶与种实表型性状变异分析

Analysis on the Phenotypic Variation of Needles and Seeds of Picea brachytyla Natural Population

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