四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (04): 384-390.doi: 10.16036/j.issn.1000-2650.2020.04.002

• • 上一篇    下一篇

60Co-γ射线辐照藜麦的效应及适宜剂量初步研究

蒋云1,2, 唐力为3, 张洁1, 李健伟1, 彭宇1, 宣朴4, 王颖1, 郭元林1,*   

  1. 1.四川省农业科学院生物技术核技术研究所,成都 610061;
    2.四川农业大学小麦研究所,成都 611130;
    3.攀枝花市农林科学研究院,四川 攀枝花 617000;
    4.四川省农业科学院农产品加工研究所,成都 610066
  • 收稿日期:2019-11-04 出版日期:2020-08-28 发布日期:2021-02-02
  • 作者简介:蒋云,副研究员。*责任作者:郭元林,副研究员,主要从事作物遗传育种研究,E-mail:1214199456@qq.com。
  • 基金资助:
    四川省科技厅应用基础研究项目(19YYJC1703); 攀枝花市农林科学院院立项目(2017nly02); 干热河谷特色生物资源开发四川省高校重点实验室项目(GR-2018-C-01)

Primary Study on Suitable Dose and Effects of 60Co-γ Ray Irradiation on Quinoa

JIANG Yun1,2, TANG Liwei3, ZHANG Jie1, LI Jianwei1, PENG Yu1, XUAN Pu4, WANG Ying1, GUO Yuanlin1,*   

  1. 1. Sichuan Academy of Agricultural Sciences Biotechnology and Nuclear Technology Research Institute,Chengdu 610061,China;
    2. Sichuan Agriculture University Triticeae Research Institute,Chengdu 611130,China;
    3. Panzhihua Academy of Agriculture and Forestry Sciences,Panzhihua 617000,Sichuan,China;
    4. Sichuan Academy of Agricultural Sciences Institute of Agro-products Processing Science and Technology,Chengdu 610061,China
  • Received:2019-11-04 Online:2020-08-28 Published:2021-02-02

摘要: 【目的】为了了解藜麦的辐照效应,初步探讨60Co-γ射线辐射诱变藜麦的适宜剂量。【方法】用60Co-γ射线对4份藜麦材料(NX-1,CX-2,SHX-8和SHX9)的干种子进行了7个剂量梯度的辐照处理,对辐照当代种子的发芽率、苗高、成活率、株高、穗长、茎粗、千粒重和籽粒粗蛋白含量进行了调查和分析。对NX-1的M2代群体进行了选育和考种。【结果】不同藜麦材料间和不同剂量间的辐照效应均存在差异,参试藜麦的半致死剂量为84.2~152.4 Gy,平均116.2 Gy,致死剂量均低于320 Gy;随辐照剂量增大,发芽率、苗高、株高、穗长显著降低;千粒重、茎粗变化不明显;籽粒粗蛋白含量增加。辐照M1代中,剂量、苗高、发芽率、成活率、穗长、籽粒粗蛋白含量等性状间大多具有显著或极显著的相关性,千粒重、茎粗与其他性状相关性不显著。M2代中选单株主要来自80 Gy和160 Gy的后代,平均单株产量和蛋白质含量比NX-1原始种质有一定提高。【结论】60Co-γ射线用于藜麦诱变育种的适宜剂量可能是平均半致死剂量(116.2 Gy)为中心的一个范围,在实践中,可采用本研究提出的平均半致死或更低的剂量进行辐照处理。本研究可为进一步开展藜麦的辐照诱变育种提供参考。

关键词: 藜麦, 藜麦, 诱变育种, 诱变育种, 适宜剂量, 适宜剂量, 半致死剂量, 半致死剂量, 60Co-γ射线, 60Co-γ射线

Abstract: 【Objective】 Preliminary evaluate the irradiation effect and finger out the suitable doseage of quinoa. 【Method】 Dry seeds of 4 accessions of Chenopodimu quinoa willd (NX-1, CX-2, SHX-8 and SHX-9) were used in the present study and exposed to 7 dosage classes (40、 80、 160、 240、 320、 640 and 1 280 Gy) 60Co-γ ray. In M1 generation, 9 agricultural traits inculding seed germination rate, seedling height, survival rate, plant height, spike length, stem diameter, thousand kernel weight and kernel crude protein content were investigated and analyzed. The M2-generation derived from NX-1 was selected and investigated. 【Result】 In terms of irradiation effect, there were significant differences among 4 accessions as well as 7 dosage groups. The semi-lethal dose of 4 accesstions of tested quinoa were between 84.2 Gy to 152.4 Gy, and mean value was 116.2 Gy. The lethal dose were less than 320 Gy. Higher dosages of γ-irradiation resulted in an significant decrease in all investigated traits with the exception of thousand kernel weight and stem diameter. In M1 generation, all observed traits showed significant or extremely significant correlation with each other, with the exception of thousand kernel weight and stem diameter. The selected plant in M2 generation mainly came from the offspring population of 80 Gy and 160 Gy. The average yield per plant and crude protein content of selected plant were improved to some certain extent against NX-1 original varieties. 【Conclusion】 The optimal dose of 60Co-γ ray for mutagenesis breeding of quinoa may be a range centered on the mean half-lethal dose (116.2 Gy). In breeding practice, the average semi-lethal or lower dose was the recommendation for irradiation treatment. This study provided preliminary reference for radiation induced mutagenesis in quinoa in the further.

Key words: Quinoa, Quinoa, mutation breeding, mutation breeding, suitable dose, suitable dose, semi-lethal dose 60Co-γ ray, semi-lethal dose 60Co-γ ray

中图分类号: 

  • S519