四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (01): 105-112.doi: 10.16036/j.issn.1000-2650.2020.01.016

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和厚朴酚对斑马鱼(Danio rerio)的急性毒性病理损伤评估

熊关庆1,2, 冯杨2, 杨玉涔2, 段惠民2, 黄彦博2, 李泉汐2, 李志洪2, 石存斌1,*, 汪开毓3, 黄小丽2,*   

  1. 1.中国水产科学研究院珠江水产研究所/农业农村部渔用药物创制重点实验室/广东省水产动物免疫技术重点实验室,广州 510380;
    2.四川农业大学动物科技学院水产系,成都 611130;
    3.四川农业大学动物医学院,成都 611130
  • 收稿日期:2019-09-26 出版日期:2020-02-28 发布日期:2021-02-02
  • 作者简介:熊关庆, 硕士研究生。*责任作者:石存斌,研究员,主要从事水产动物病害防治研究, E-mail:shicunbin2006@163.com;黄小丽, 副教授,主要从事水生动物病害学研究, E-mail:hxldyq@126.com。
  • 基金资助:
    广东省水产动物免疫技术重点实验室开放基金项目 (201502)

Evaluation of Acute Toxicologic Pathology Following Immersion of Honokiol in Zebrafish (Danio rerio

XIONG Guanqing1,2, FENG Yang2, YANG Yucen2, DUAN Huimin2, HUANG Yanbo2, LI Quanxi2, LI Zhihong2, SHI Cunbin1,*, WANG Kaiyu3, HUANG Xiaoli2,*   

  1. 1. Pearl River Fishery Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Drug Development,Ministry of Agriculture and Rural Affairs/Key Laboratory of Aquatic Animal Immune Technology,Guangdong Province,Guangzhou 510380,China;
    2. Department of Aquaculture,College of Animal Science & Technology,Sichuan Agricultural University,Chengdu 611130,China;
    3. College of Veterinary Medicine, Sichuan Agricultural University,Chengdu 611130,China
  • Received:2019-09-26 Online:2020-02-28 Published:2021-02-02

摘要: 【目的】针对和厚朴酚(Honokiol)对斑马鱼(Danio rerio)的毒性损伤,采用半静态急性毒性实验将斑马鱼浸泡于和厚朴酚溶液中,研究和厚朴酚对斑马鱼的毒性作用及组织病理学影响。【方法】将105尾健康斑马鱼随机浸泡于7个试验组中(n=15):对照组(曝气自来水)、助溶剂乙醇组(1%体积比)和5个和厚朴酚浓度组(0.1、0.2、0.4、0.6、和0.8 mg/L),对斑马鱼的临床症状、大体病变、死亡率以及组织病理学进行判定,并结合组织病理评分系统评价各组斑马鱼的损伤情况,评估和厚朴酚的毒性。【结果】不同浓度和厚朴酚组浸泡的斑马鱼在96 h内出现不同程度死亡,其96 h半数致死浓度(LC50)为(0.530±0.014) mg/L,72 h-LC50为(0.679±0.013) mg/L,48 h-LC50为(0.758±0.012) mg/L,24 h-LC50为(0.816±0.01) mg/L,而安全浓度(SC)为(0.264±0.003) mg/L。此外,在设定的浓度范围内,和厚朴酚未造成斑马鱼出现明显的临床及大体病理变化。组织病理学显示,和厚朴酚浸泡后主要导致鳃和脑充血,此外还可见鳃呼吸上皮坏死等,其余组织与对照组相比无明显病变。【结论】和厚朴酚对于斑马鱼存在一定毒性,鳃和脑可能是和厚朴酚对鱼类的主要毒性靶器官。

关键词: 和厚朴酚, 斑马鱼, 急性毒性损伤, 毒性病理学研究

Abstract: 【Objective】 To evaluate the toxicity of honokiol to zebrafish (Danio rerio), the present study builds a semi-static acute toxicity test to assess the mortality and histopathology of zebrafish immersed in honokiol solution. 【Method】 After pre-experimental determination of the highest non-lethal concentration and minimum lethal concentration of honokiol, 105 healthy zebrafish were randomly immersed into 7 experimental groups (n=15), including control group (aerated tap water), ethanol solvent group (1% by volume) and 5 experimental groups (0.1, 0.2, 0.4, 0.6 and 0.8 mg/L). The gross lesions, mortality, and histopathology of zebrafish were determined, and the damage of zebrafish in each group was evaluated by the histopathological scoring system. 【Result】 Zebrafish immersed in different honokiol groups showed different degrees of death within 96 h, 96 h-LC50 was (0.530±0.014) mg/L, 72 h-LC50 was (0.679±0.013) mg/L,48 h-LC50 was (0.758±0.012) mg/L, 24 h-LC50 was (0.816±0.01) mg/L, and the safety concentration (SC) was (0.264±0.003) mg/L. In addition, honokiol did not cause a significant gross pathological loss in zebrafish. The histopathology results showed that the soaking of magnolol and magnolol mainly caused blood congestion in the gills and brain. Additionally, the respiratory epithelium necrosis of gills was also observed. The remaining tissues showed no obvious lesions compared to the control group. 【Conclusion】 According to the results of toxicity pathology, gill might be the main toxic target tissue of honokiol in fish. It is hoped the present results will provide references for the development of related drugs.

Key words: honokiol, Danio rerio, acute toxicity injury, toxicologic pathology

中图分类号: 

  • S948