关键词: 红螯螯虾, 转录组, 高通量测序

Abstract: 【Objective】 To excavate and study the functional genes and SSR markers of red crayfish.【Method】 A new generation of high-throughput sequencing technology, Illumina Hi Seq 2000, was used to sequence the liver, testis, and ovary transcriptomes of Cherax quadricarinatus, and gene expression profiling and functional gene prediction were performed using bioinformatics methods. 【Result】 47 629 414 clean reads were obtained in the liver, 47 018 968 clean reads were obtained in the testes,and 53 267 362 clean reads were obtained in the ovarian tissues. After all reads were assembled, 67 369 Unigenes were obtained. Classified by GO, 16 989 Unigenes are divided into three categories, namely biological processes, cellular components, and molecular functions. By COG classification, 4 697 Unigenes are divided into 25 categories. By KEGG classification, 9 842 Unigenes belong to 331 metabolic pathways. This study lays a certain theoretical foundation for the better development and utilization of the resources of the red chelonian crayfish. 【Conclusion】 Gene expression profiling and its functional genes of C. quadricarinatus were obtained by using high-throughput sequencing technology.

Key words: Cherax quadricarinatus, transcriptome, high-throughput sequencing