四川农业大学学报 ›› 2005, Vol. 23 ›› Issue (01): 104-108.doi: 10.16036/j.issn.1000-2650.2005.01.022

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HMW-GS基因的遗传转化与小麦品质改良

李琴, 武丽敏, 江千涛, 魏育明, 颜泽洪, 郑有良   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2004-12-20 出版日期:2005-03-31 发布日期:2017-04-21
  • 基金资助:
    高等学校全国优秀博士学位论文作者专项资金(200357);国家自然科学基金项目(30300219)。

Genetic Transformation of HMW-GS Genes into Wheat and Its Potential in the Improvement of Wheat Quality

LI Qin, WU Li-min, JIANG Qian-tao, WEI Yu-ming, YAN Ze-hong, ZHENG You-liang   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2004-12-20 Online:2005-03-31 Published:2017-04-21

摘要: 小麦高分子量谷蛋白亚基(HMW GS)的组成类型及相对含量与面筋的弹性和强度直接相关,决定面粉的烘烤品质。目前,应用转基因技术将HMW GS基因(主要为1Ax1、1Dx5和1Dy103个亚基基因)导入普通小麦,获得转基因株系。对转基因株系进行检测,发现导入的HMW GS基因在转化植株中均过量表达。对其品质进行测定表明,面团弹性、强度、稳定时间等多个品质性状都有所改善。我国小麦品种品质普遍较差,利用转基因技术提高优质亚基的表达数量,转入外源优质亚基,可能将是我国小麦,尤其是南方小麦品质遗传改良的有效途径之一。

关键词: 高分子量谷蛋白亚基, 转基因, 小麦, 品质

Abstract: The composition and quantity of high molecular weight glutenin subunits (HMW-GS) determine the gluten elasticity and strength, and influence the baking quality of wheat. Several HMW-GS genes, such as 1Ax, 1Dx5 and 1Dy10, have been transformed into wheat cultivars. By evaluating transformed lines with 1Ax, 1Dx5 and 1Dy10, it is indicated that the transgenes are overexpressed and quality properties of transgenic wheat lines, such as gluten elasticity, strength and stability time, have been significantly improved. By incorporating alien HMW-GS or the exploiting the overexpressed HMW-GS, transgene technique is probably one of the potential approaches to improve the quality of Chinese wheat, especially the wheat cultivars in southern China.

Key words: HMW-GS, transgene, wheat, quality

中图分类号: 

  • S512.1