四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (01): 52-55.doi: 10.3969/j.issn.1000-2650.2011.01.010

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不同种源引种紫锥菊的过氧化物酶同工酶分析

韩琳娜, 周凤琴   

  1. 山东中医药大学药学院, 济南 250355
  • 收稿日期:2010-11-25 出版日期:2011-03-31 发布日期:2017-04-11
  • 通讯作者: 周凤琴(E-mail:zfqsdzy@yahoo.com.cn) E-mail:zfqsdzy@yahoo.com.cn
  • 基金资助:
    山东省高校科技计划项目(J10LF77)

The POD Isozyme Analysis of Introduced Echinacea purpurea from Different Provenance

HAN Lin-na, ZHOU Feng-qin   

  1. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Received:2010-11-25 Online:2011-03-31 Published:2017-04-11

摘要: 采用过氧化物同工酶技术首次对我国8个种源引种紫锥菊的遗传多样性进行分析,并通过排序分析其相互之间的亲缘关系。结果表明,不同种源紫锥菊的POD同工酶谱在酶带数、迁移率及酶量等方面存在比较明显的差异,具有较丰富的遗传多样性。临沂引种紫锥菊与其他7个种源的不相似值最大,长沙引种紫锥菊和怀柔引种紫斑茎紫锥菊亲缘关系较近,怀柔引种绿茎紫锥菊和怀柔引种紫茎紫锥菊酶谱特征完全相同。POD同工酶谱可以作为不同种源紫锥菊遗传多样性鉴定的参考依据。

关键词: 引种紫锥菊, 过氧化物酶同工酶, 亲缘关系, 遗传多样性

Abstract: The genetic diversity of introduced Echinacea purpurea, from 8 different provenance were analyzed by using the technique of peroxidase(POD) isozymes.The sibship among them was studied by index analysis.The results show that obvious differences exist among them in the number of bands, Rf and amount of POD isozyme.Introduced E. purpurea from Linyi is distantly related to other 7 species. E. purpurea from Changsha is close to purple spots stem species from Huairou.Green stem species and purple stem species from Huairou have the same enzyme spectrum.Thus, peroxidase isozyme patterns can be a useful marker for genetic diversity of Echinacea purpurea from different provenance.

Key words: introduced Echinacea purpurea, peroxidase isoenzymes, sibship, genetic diversity

中图分类号: 

  • S565