关键词: 离体培养[49DB], 外植体建立, 樱花(Prunus serrulata)

Abstract: Well-developed buds were isolated in June from two-year-old Japanese late cherry trees(P. serrulata var. lannesiana) grafted on Chinese cherry rootstocks. Primordial shoots of about 0.2 cm in size were excised as explants. The use of low-strength soft initiation medium(1/4MS+2% sucrose+0.5% agar, supplemented with 0.5mg/L BAP and O. 05mg/L IBA) was effective in preventing browning of the explants. It was essential for freedom from waterlogging to culture explants on amedium surface free of liquid film and maintain the explants free of being wetted. An appropriate concentration of BAP(1-2mg/L) in combination with low concentration of IBA(0.05mg/L) could minimize the occurence of vitrescence. Repeated transferring at about three week interval, helped overcome problems of vigor decline and rosetted shoots. After about three months of transferring, vigor decline eventually became less apparent and shoots could elongate about 2 cm on a fresh medium without GA₃ whereas stems of 4-5cm in length could be obtained with the addition of 2mg/L GA₃ to the medium.

Key words: CULTURE IN VITRO, ESTABLISHMENT OF EXPLANTS, JAPANESE FLOWERING CHERRY(P. ERRULATA VAR. LANNESIANS)