关键词: 水稻[49EJ], 过氧化物酶[45EC], 减压渗透法, 外源DNA, 同工酶[45EC]

Abstract: By using polyacrylamide gel electrophoresis(PAGE) and polyacryla-mide gel isoelectric focusing(PAG-IEF). analysis on isoperoxidase was carried out for variant desendants obtained from transmitting isolated DNA of Triticum aestivum L., Zea may L., Setaria italica(L.) Beau V., Serghum vulgare Pers., Pennistum sp. into purple rice by decompress osmosis, a new plant molecular breed-ing technique. in four different growth stage, seedling, tillering, panicie formation and-heading. The results showed that isoperodases patterns were differert in a few weak and trace bands between purple rice and all variant descendants in the tested grawth stage, which indicated that the variants possess genetic base different that of the purple rice. In the end. the authors suggest that IEF be a powerful method for identifing genetic differences in plant.

Key words: PADDY, PEROXIDASE, DECOMPRESS OSMOSIS, EXOGENOUS DNA, ISOENZYMES