关键词: 作物遗传育种, 玉米[45CJ, 49EJ], 自交系[49DA], 姊妹系, 姊妹种, 同功酶[45EC], 电泳[32KA, 34BC, 45AH, 65CH]

Abstract: For 16 maize sib-lines and sib-crosses of self-bred line 48-2, the peroxidase and esterase isozyme patterns were analyzed with polyacrylamide vertical gel electrophoresis and the peroxidase isozyme patterns were analyzed with isoelectrec focusing. The results indicated that, among sib-lines, among sib-crosses, between the sib-line and its sib-crosses, the patterns of esterase isozyme made few differences, but the patterns of peroxidase isozyme made obvious differences. For peroxidase patterns, the enzyme belts with isoelectrec focusing were more than that with polyacrylamide vertical gel electrophoresis. So, the differences of the results with isoelectrec focusing were more apparent than those with polyacrylamide vertical gel electrophoresis.

Key words: CEREAL GENETIC BREEDING, MAIZE, SELFED LINES, SIB-LINE, SIB-CROSS, ISOENZYMES, ELECTROPHORESIS