四川农业大学学报 ›› 2003, Vol. 21 ›› Issue (02): 132-134.doi: 10.16036/j.issn.1000-2650.2003.02.015

• 研究论文 • 上一篇    下一篇

免疫吸附对新月弯孢菌抗血清非特异性抗体阻断作用研究

宁红1, 秦蓁1, 王丽焕2, 高荣2   

  1. 1. 四川省农业厅植物检疫站, 四川 成都 610041;
    2. 四川大学 生命科学院, 四川 成都 610064
  • 收稿日期:2002-11-15 出版日期:2003-06-30 发布日期:2017-03-06
  • 基金资助:
    四川省科技厅"1999年四川省重点科学技术研究项目"。

Studies on Blochade of Immunoadsorption to the Non-specific Antibodies of Curvularia lunata

NING Hong1, QIN Zhen1, WANG Li-huan2, GAO Rong2   

  1. 1. Sichuan Phytosanitary Station, Chengdu 610041, Sichuan, China;
    2. Life Science College of Sichuan University, Chengdu 610064, Sichuan, China
  • Received:2002-11-15 Online:2003-06-30 Published:2017-03-06

摘要: 用新月弯孢菌Curvularia lunata(Wakke)Boed可溶性蛋白质作为免疫抗原免疫家兔,获得兔抗C. lunata抗血清(PAbs);抗血清经近似菌可溶性蛋白质吸附后获得对C. lunata菌株可溶性蛋白质具有特异性的抗血清(APAbs),可应用于C. lunata菌株的鉴定;用玉米种子可溶性蛋白质对APAbs进行进一步吸附纯化,获得了与玉米种子蛋白质无交叉反应的特异性抗血清(DAPAbs),可用于对带菌玉米种子的检测鉴定。

关键词: 新月弯孢菌, 抗体, 免疫吸附, 阻断

Abstract: Mixed proteins of C. lunata were used as the antigens.Three kinds of primary antibodies, polyclonal antibodies(PAbs) to C. lunata, adsorbed PAbs(APAbs) with the similar fungi proteins and double adsorbed PAbs(DAPAbs) with the fungi and maize proteins were used to evaluate their detecting sensitivities and specificities.APAbs is specific to C. lunata and DAPAbs is specific to C. lunata too when reacting with the proteins of maize.DAPAbs can be used to detect the maize seed bring C. lunatap.

Key words: Curvularia lunata, antibody, immunoadsorption, blockade

中图分类号: 

  • S435.131