关键词: 小麦, 簇毛麦, 谷蛋白, 醇溶蛋白, 电泳

Abstract: In order to investigate the introgression of Dasypyrum villosum (L. ) Candargy germplasm into wheat, glutein and gliadin compositions of 151 stable wheat lines deriving from the progenies between D. villosum and common wheat variety ‘Mianyang 11’ were analyzed by seed storage protein electrophoresis. The results indicated that glutein subunits of D. villosum were not found, but there were a lot of new HWM-GS subunits or recombinated HWM-GS subunits in these wheat lines. Partial loci of HMW-GS were in heterogeneous condition, suggesting further selection in the progenies of these advanced lines. Meanwhile, some characteristic gliadin bands from D. villosum and new gliadin bands were also expressed in regions of α, β, γ, ω in these wheat lines, indicating some genes have been transferred from D. villosum into wheat. The cluster analyses show ed that there were abundant variations at gliadin loci between tested lines and their parents. Therefore, the desirable genes might be transferred from alien species into common wheat, and new variations could also be produced by wide crosses.

Key words: wheat, D. villosum, glutein, gliadin, electrophoresis