四川农业大学学报 ›› 2010, Vol. 28 ›› Issue (04): 411-414.doi: 10.3969/j.issn.1000-2650.2010.04.004

• 研究论文 • 上一篇    下一篇

提莫菲维小麦高分子量谷蛋白亚基遗传多样性分析

徐黎黎, 钟美玉, 李伟, 魏育明   

  1. 四川农业大学小麦研究所, 四川 温江 611130
  • 收稿日期:2010-11-22 出版日期:2010-12-31 发布日期:2017-03-01
  • 通讯作者: 魏育明(E-mail:yuming71@hotmail.com) E-mail:yuming71@hotmail.com
  • 基金资助:
    四川省青年科技基金项目(2010JQ0046)

Genetic diversity of High Molecular Weight Glutenin Subunits in Triticum timopheevii Zhuk.

XU Li-li, ZHONG Mei-yu, LI Wei, WEI Yu-ming   

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China
  • Received:2010-11-22 Online:2010-12-31 Published:2017-03-01

摘要: 为了给普通小麦的品质改良提供基础材料,并了解提莫菲维小麦的HMW-GS组成情况,利用SDS-PAGE技术对43份提莫菲维小麦高分子量谷蛋白亚基遗传多样性进行了分析,分别发现2、3、2和1种Ax、Ay、Gx和Gy亚基的等位变异类型和5种组合类型[Ax2*,Gx(A);Ax2*+Ay(C),Gx(B)+Gy;Ax1+Ay(B),Gx(B)+Gy;Ax1+Ay(A),Gx(A);Ay(A),Gx(B)]。其中Ax2*,Gx(A)的分布频率最高,达81.40%。在43份材料中,有12份材料(27.90%)的Ay亚基表达,Gx亚基几乎都表达,而只有部分Gy亚基可表达,但频率非常低,仅为6.98%。结果表明提莫菲维小麦Glu-A1的遗传多样性高于Glu-G1。文中对检测到的各种亚基的利用方式进行了讨论。

关键词: 提莫菲维小麦, 高分子麦谷蛋白亚基, 遗传变异

Abstract: In order to provide basic materials for the quality improvement and detect the HMW-GS components of Triticum timopheevii,43 T.timopheevii were evaluated by means of SDS-PAGE.There were 2,3,1 and 2 different Ax,Ay,Gx and Gy subunits,and 5 HMW-GS combinations [Ax2*,Gx (A);Ax 2*+Ay (C),Gx (B)+Gy;Ax1+Ay (B),Gx (B)+Gy;Ax1+Ay (A),Gx (A);Ay (A),Gx (B)],Among which,combination Ax2*,Gx (A),accounting for 81.40% of the total T.timopheevii accessions,presented most frequently.The expressed Ay and Gy subunits were detected in T.timopheevii accessions,amounting to 27.90% and 6.98%,respectively.These results indicated that the Glu-A1 had higher genetic diversity than the Glu-G1.The utilization strategy of these subunits was discussed.

Key words: T.timopheevii, HMW-GS, genetic variation

中图分类号: 

  • S512