关键词: 绿粘帚霉, 原生质体, 制备, 再生

Abstract: The conditions of protoplast preparation and regeneration were studied on Gliocladium virens (F051)by means of one way experiment.The optimal conditions of protoplast preparation and regeneration of Gliocladium virens (F051)were showed as follows:the optimum conditions of F051 protoplast isolation and the regeneration are:conidiophores cultured for 24 h by using culture medium A,0.6 mol/L MgSO4 (regeneration used 0.6 mol/L sucrose)as osmotic stabilizer,the sodium hydrogen phosphate citric acid as the buffer system,the cellulase:Snailase:Lysozyme=4 mg/mL:2 mg/mL:2 mg/mL,in 32℃ water baths dige-sting 2 h.

Key words: Gliocladium virens, protoplast, production, regeneration