四川农业大学学报 ›› 2008, Vol. 26 ›› Issue (02): 125-130.doi: 10.16036/j.issn.1000-2650.2008.02.006

• 研究论文 •    下一篇

小麦族赖草属植物与近缘二倍体物种的同工酶分析

钟珉菡1, 苟琳1, 刘静1, 周永红2, 杨瑞武1   

  1. 1. 四川农业大学 生命科学与理学院, 四川 雅安 625014;
    2. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2007-12-14 出版日期:2008-06-30 发布日期:2017-02-28
  • 基金资助:
    国家自然科学基金(30670150);四川省青年科技基金(07JQ0085)

Isozyme Analysis of Species of Leymus and its Related Diploid Species in Triticeae

ZHONG Min-han1, GOU Lin1, LIU Jing1, ZHOU Yong-hong2, YANG Rui-wu1   

  1. 1. College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2007-12-14 Online:2008-06-30 Published:2017-02-28

摘要: 对小麦族赖草属、冰草属、偃麦草属、澳冰草属、拟鹅观草属、新麦草属和大麦属7个属24个物种的酯酶同工酶和超氧化歧化酶同工酶进行比较分析。结果表明:7个属24个物种共出现24种不同的同工酶酶谱,电泳分离出迁移率不同的45条酶带;②Leymus chinensisLeymus其他13个种的亲缘关系较远,与Hordeum bogdanii的亲缘关系较近,Psathyrostachys junceaLeymus的亲缘关系很近;③相似染色体组的物种聚类在一起;④2种同工酶的分析结果与细胞学、形态学及分子标记的研究结果基本一致。

关键词: 赖草属, 同工酶, 酯酶, 超氧化物歧化酶, 聚类分析

Abstract: The esterase isozyme (EST)and superoxide isozyme (SOD)of 24 species of Leymus,Australopyrum,Agropyron,Lophopyrum,Pseudoroegneria,Psathyrostachys and Hordeum in triticeae were analyzed by using polyacrylamid gel electrophoresis (PAGE)technique.The results show as follows:①All the 24 species have significant isozymes polymorphism,with a total of 45 bands separated;②The relationship between L. chinensis and the other 13 species of Leymus is remote,while it is close to Hordeum bogdanii.Psathyrostachys juncea and the species of Leymus have closer relationship;③The species of those that contain similar genomes are clustered together;④The results of isozyme analysis are basically consistent with those obtained from studies on cytology and morphology.

Key words: Leymus, isoenzyme, EST, SOD, cluster analysis

中图分类号: 

  • Q949.714.2