关键词: 睾丸, 附睾, T细胞, 公鸡

Abstract: [Objective] The aim of this study was to localize the distribution of CD4 and CD8 positive T cell in reproductive organs of rooster. [Method] The expression level of IL-8was examined by qRT-PCR,and CD4+/CD8+T cells were localized by immunohistochemistry on cyro-sections. [Results] IL-8had a high expression in epididymis,and had a low expression in testis.Moreover,a few of CD4+/CD8+T cells were localized in the region of interstitial cell,and CD4+/CD8+T cells were localized in mucosal epithelium and lamina propria of the epididymal duct and duct defense.Moreover,the density change of CD4+/CD8+T cells in different segments of reproductive organs have significant difference,the density of CD4+/CD8+T cells in middle segment (efferent duct and epididymal duct)was significantly higher than that the two terminals (rete testis,and duct deferens). [Conclusion] The results suggested epididymis,especially efferent duct and epididymal duct were the main segment of immunodefense by cell-mediated immunity.

Key words: testis, epididymis, T cells, rooster