关键词: 玉米, 转基因, 植物激素, 组织培养

Abstract: [Objective] This study optimized the transgenic system based on the acceptor of maize inbred line 18-599 through adding different kinds of plant hormones at different concentration into the medium,and aimed to facilitate large-scale of transgenic breeding.[Method] In this study,maize inbred line 18-599,a key inbred line in southwest,was performed as the transgenic receptor and N₆/MS medium was used as the base medium.Several mediums modified by adding plant hormones including KT,ABT and S-3307 were compared in terms of callus differentiation,seedling vigor and root generation.Meanwhile,the survival rate of transgenic seedlings was improved when exposed to KMnO₄ in short time.[Results] The results suggested that N6 medium containing 0.5 mg/L KT could improve the frequency of callus differentiation.N6 medium containing1.0 mg/L ABT and 1.0 mg/L IBA significantly increased root regeneration up to 80%.Modified N6 medium containing 0.5 mg/L S-3307 could stimulate the growth of root development and make seedlings dwarf and vigor.The survival rate of the transgenic seedlings can be raised up to 74.2% when subjected to short time treatment of KMnO₄.[Conclusion] In this study,treatment including adding suitable plant hormone in the medium could enhance callus differentiation,promote root growth,and improve seedling vigor.Meanwhile,exposing the seedling to low concentration KMnO₄ on transplanting facilitated the survival rate of the transgenic seedlings.These results showed that the genetic transformation system based on the acceptor of maize inbred line 18-599 was further optimized in this study.

Key words: maize, transgenic engineer, plant hormone, tissue culture