关键词: 梅花鹿, LHβ基因, 克隆, 序列分析

Abstract: [Objective] The aim of this study is to clone the LHβ gene CDS of Cervus nippon and analyze the structure and features of LHβ protein. [Method] The LHβ gene CDS of Cervus nippon was obtained by PCR, cloning and sequencing. Then, the structure and features of LHβ protein, including molecular weight, isoelectric point, secondary structure, function were predicted by online molecular biological tool, such as ProtParam tool. Meanwhile, we constructed a phylogenetic tree by MEGA 6.0software to further explain the genetic basis for this gene. [Results] The full CDS sequence of Cervus nippon LHβ gene was 462 bp(GenBank Accession No. KT199365), encoding 141 amino acids, which contained ATG start codon, and TGA stop codon, with a calculated molecular weight of 15.17 kDa and an isoelectric point of 8.00. No conserved transmembrane region was detected and all of the amino acids were located outside the membrane. The average of hydrophobicity(GRAVY) was 0.391. Function predicted results showed that the function of this protein most probable act as a hormone, which was in agreement with the target gene. The phylogenetic analysis showed that Cervus nippon had close affiliation with ruminant, including Capra hircus, Ovis aries and Bos taurus, especially Ovis aries. [Conclusion] The LHβ gene CDS of Cervus nippon had the closest affiliation with Ovis aries.

Key words: Cervus nippon, LHβ gene, cloning, sequence analysis