关键词: 安乃近, 牛血清白蛋白, 相互作用, 金属离子

Abstract: [Objective] The interaction of Analgin(ANG) with bovine serum albumin(BSA) was investigated so as to provide references for its clinical application. [Method] Under the optimal conditions, the interaction of ANG with BSA and the effect of coxisting metal ions were investigated by spectrometry. [Results] The results showed that the fluorescence of BSA was quenched by ANG. It was a static quenching process. BSA could transport ANG, which was spontaneous and mainly driven by Der Waals forces or hydrogen bond. The primary binding site for ANG was located at sub-domain ⅡA of BSA, which was near by tyrosine residue. There was some negative cooperative effect. The conjugation reaction could affect the conformation of BSA, which led to the polarity around BSA strengthened. Mn²⁺, Fe³⁺, Cr³⁺, Zn²⁺ and Mg²⁺ competed with the interaction, which could increasing medical effectiveness. [Conclusion] ANG can interact with BSA and the interaction was affected by metal ions.

Key words: Analgin, bovine serum albumin, interaction, metal ions