关键词: 硫酸盐还原菌, 培养条件, 菌剂

Abstract: [Objective] The main factors of the growth of two Sulfate-Reducing Bacterium(SRB) strains were investigated and preliminary trail of SRB microbial inoculum was carried out. [Methods] The impact of pH, temperature, carbon source and culture media on the growth of SRB were studied by the method of CFU for strain density count, moreover, the trial-produce of SRB4 microbial inoculum was implemented. [Results] The optimum culture conditions for SRB4 were as follows:temperature 38℃; pH 8.0; provided Postgate medium with sodium citrate as carbon source. The optimum culture conditions for SRB3 were as follows:temperature 36℃; pH 7.0; Postgate medium with the water extract of corn cob as carbon source. The strain density of SRB4 was maintained above 10¹² CFU/mL after 48 h fermentation. [Conclusion] Under the optimal conditions, the strain density of the two SRBs were up to 10¹² CFU/mL, and they could be alternative strains for engineering application.

Key words: sulfate reducing bacteria, culture conditions, microbial inoculum