关键词: 黄花菜, 多糖, 正交试验, IL-2, TNF-α

Abstract: [Objective] This experiment was to study the optimal extraction technology and anti-tumor effect of polysaccharide in Hemerocallis fulva. [Methods] Determination of the content of polysaccharide in Hemerocallis fulva by phenol-sulfuric acid method. On the basis of single factor experiment, L₉(3⁴) orthogonal design was adopted to study the effects of extraction times, extraction temperature, extraction time and solid-liquid ratio on yield of polysaccharide. Using S₁₈₀ tumor-bearing mice as model we observed mice transplanted tumor inhibition rate. The levels of IL-2 and TNF-α in serum were detected by antibody sandwich enzyme-linked inununosorbent assay(ELISA). [Results] The results showed that the optimal extraction process parameters were as followed:ratio of solid-liquid 1:25(g:mL), ex-traction temperature 80℃, extraction time 2 h, extracting 3 times. Under such conditions, the average extraction rate of polysaccharide in Hemerocallis fulva was 17.47%. Polysaccharides in Hemerocallis fulva had inhibited the growth of S180 transplanted tumor. The rate of inhibition was 38.54% in high-er-doses groups(1.60 g/kg). Polysaccharides in Hemerocallis fulva can improve contents of IL-2 and TNF-α in serum of mice with tumors. [Conclusion] Polysaccharides in Hemerocallis fulva have strong antitumor activity. The mechanism may relate to enhanced of immunifaction.

Key words: Hemerocallis fulva, polysaccharide, orthogonal design, IL-2, TNF-α