关键词: 柏木木屑；柏木精油；成分；抑菌活性；抗氧化活性

Abstract:

【Objective】Essential oil in C. funebris chips was extracted and optimizeresource utilization and sustainable development of C. funebris can be realized. 【Method】The essential oil was extracted by steam distillation and oil components were separated and identified by gas chromatography-mass spectrum (GC-MS). The antibacterial effects were investigated using filter paper diffusion methods when essential oil and streptomycin used alone and in combined with each other. The reducing power and scavenging capacity against 1,1- streptomycin, Diphenyl-2-picrylhydrazyl and hydroxyl and superoxide anion free radical antioxidant activities were measured. 【Results】The essential oil of C. funebris mainly contained cedrol (26.06%), thujopsene(18.25%), cuparene(15.06%), α-cedrene(8.23%), α-curcumene(3.20%), β-Caryophyllene(2.83%), β-Himachalene(2.69%) and (+)-β-Cedrene(2.48%). ,Essential oil alone was highly sensitive to Salmonella and moderately sensitive to the other tested strains.  In Escherichia coli, Enterobacter hormaechei, Bacillus anthraci and Klebsiella antibacterial experiments, streptomycin play a major role. While in Salmonella and Bacillus subtilis bacteria experiments, essential oils play a major effect. The antioxidant activity of essential oil increased with the increase in concentration, exhibiting stronger reducing power. The half maximal inhibitory concentrations (IC50) against 1, 1-Diphenyl-2-picrylhydrazyl, hydroxyl and superoxide anion free radical were 2.55, 2.23 and 1.30 mg/mL, respectively, indicating stronger antioxidant activity.【Conclusion】The essential oil from C. funebris has strong antibacterial and antioxidant capacity, which provides a theoretical basis for the study of biological fungicides and natural food preservatives and antioxidants.

Key words: Cupressus funebris chip; Essential oil of C. funebris; components; antibacterial activity; antioxidant activity