四川农业大学学报

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石竹(Dianthus chinensis) 对土壤镉胁迫的生理生化响应

  

  1. 1.四川农业大学风景园林学院,四川 成都 611130; 2.长江三峡实业有限公司,湖北 宜昌 443002; 3.西南林业大学林学院,云南 昆明 650224
  • 收稿日期:2013-03-12 出版日期:2013-09-27 发布日期:2013-09-27

Physiological and Biochemical Responses of Dianthus chinensis to Soil Cadmium Stress

  • Received:2013-03-12 Online:2013-09-27 Published:2013-09-27

摘要:

【研究目的】为探讨石竹对土壤重金属Cd污染的抗性和敏感性;【方法】采用盆栽控制试验,测定不同浓度(0、0.3、1、3、10、30、50 mg/kg)Cd胁迫下石竹叶片生理生化指标;【结果】研究表明,石竹根重、地上部干重、株高、抗性系数以及可溶性蛋白含量以3 mg/kg Cd浓度为阈值呈低促高抑现象。随Cd处理浓度、时间的增加,游离脯氨酸、丙二醛含量和质膜相对透性均逐渐上升,且在高浓度(≥10mg/kg)下上升更为显著;SOD, POD和CAT酶活性呈先上升后下降趋势,上升最大值的处理浓度分别为10、3、3 mg/kg;【结论】低浓度Cd对石竹影响较小,当浓度大于10 mg/kg时,其毒性随浓度和时间的增加而明显增大,表明石竹对土壤Cd胁迫有一定适应性。

关键词: Cd胁迫;石竹;生理生化;响应

Abstract:

【OBJECTIVE】The aim of this study was to investigate the resistances and susceptibility of D. chinensis to soil heavy metal cadmium (Cd). 【METHOD】A pot-experiment was conducted to measure physiological and biochemical indicators of D. chinensis leaf grown in different Cd concentrations (0, 0.3, 1, 3, 10, 30 and 50 mg/kg). 【RESULTS】The biomass of root and shoot, plant height, resistance coefficient and content of soluble protein, increased when Ca concentration was below 3 mg/kg but the opposite was true  when Ca concentration was above 3 mg/kg. With the increase of cadmium concentrations and treatment time, free proline, malondialdehyde and relative conductivity increased gradually. The activities of SOD, POD and CAT increased initially and then declined 【CONCLUSION】Low Cd concentrations had little effects on D. chinensis. when Cd concentration>10 mg/kg, the toxicity of cadmium was increased with an increase in concentration and time, implying that D. chinensis is  relatively adaptabe to soil cadmium stress.

Key words: cadmium stress; Dianthus chinensis; physiology and biochemistry; response