关键词: 华山新麦草；AFLP标记；遗传变异

Abstract:

Abstract:【Objective】This study was used to analyze the genetic variation of PHW-SA.【Method】195 pairs of AFLP primers were used to amplify PHW-SA and its parents J-11(common wheat) and Psathyrostachys huashanica.【Result】5136, 3953 and 5637 markers were amplified in J-11, Psathyrostachys huashanica and PHW-SA, respectively, accounted for 29.0%, 22.3% and 31.8% of the total (17726 banding), respectively; 81.8% markers of J-11 and 49.6% markers of Psathyrostachys huashanica were amplified in PHW-SA; there were 2484 co-markers between PHW-SA and J-11, which accounted for 48.4% and 44.1% of themselves, respectively; similarly, 246 co-markers existed between (6.2%) PHW-SA and (4.4%) Psathyrostachys huashanica; 232 new markers which did not exist in its parents were amplified in PHW-SA and 66 markers of its parents disappeared in PHW-SA; there were 1715 co-markers in all 3 materials, accounted for 33.4%, 43.4% and 30.4% of its total markers of J-11, Psathyrostachys huashanica and PHW-SA, respectively.【Conclusion】All evidence suggesting that great genetic variation was occurred in genome in the synthesize process of PHW-SA, and providing the theoretical information for wheat breeding in future.