BAK1减弱拟南芥对Turnip crinkle virus的感病性

四川农业大学学报

BAK1减弱拟南芥对Turnip crinkle virus的感病性

杨辉

四川农业大学农学院

收稿日期:2011-09-01 修回日期:2011-10-23 出版日期:2011-12-25 发布日期:2011-12-26
通讯作者: 杨辉

BAK1 confers reduced susceptibility to Turnip crinkle virus in Arabidopsis

Hui HuiYang

College of Agriculture, Sichuan Agricultural University

Received:2011-09-01 Revised:2011-10-23 Online:2011-12-25 Published:2011-12-26
Contact: Hui HuiYang

摘要/Abstract

摘要：

本文以Turnip crinkle virus（TCV）- 拟南芥（Col-0）为互作系统，研究了接种中后期，BAK1在亲和互作中的影响。结果发现，bak1-4突变体对TCV更加敏感，叶片易褪绿枯萎；而过量表达植株在接种TCV后受损程度较小。通过H2O2检测，发现严重的病症伴随有较多活性氧积累，也进一步说明，在这个亲和互作系统中，活性氧有利于症状的形成，BAK1 对活性氧产生有负调控作用。此外，BAK1过表达有利于MAPK途径下游一些与防御相关的转录因子的激活，调节植物的免疫反应。然而诱导的水杨酸途径PR1，PR2基因的表达没有介导对TCV的抗性。

关键词: 芜菁皱缩病毒（TCV）, BAK1, 拟南芥

Abstract:

We analyzed the effect of BAK1 in the Turnip crinkle virus (TCV)-Arabidopsis thaliana Col-0 compatible interaction. As a result, loss of BAK1 function resulted in enhanced susceptibility to infection by TCV, enhanced leaves chlorosis and withered. However, BAK1 overexpressing plants delayed the disease development. We found the symptoms was associated with the reactive oxygen species (ROS) accumulation in this compatible interaction. BAK1 might negatively regulate ROS production. We also found the TCV-induced elevated expression of potential target genes encoding transcription factors downstream of the MAPKs in BAK1 transgenic plants, which played a role in the immunity response to TCV. However, the induced PR1 and PR2 expression did not confer resistance to TCV.

Key words: Turnip crinkle virus (TCV), BAK1, Arabidopsis

杨辉. BAK1减弱拟南芥对Turnip crinkle virus的感病性[J]. 四川农业大学学报, 2011, 29(04): 460-464.

Hui HuiYang. BAK1 confers reduced susceptibility to Turnip crinkle virus in Arabidopsis[J]. jsau, 2011, 29(04): 460-464.

[1]	杨辉, 黄云, 宋琴, 刘鑫. BAK1在减弱拟南芥对Turnip crinkle virus感病性作用中的影响[J]. 四川农业大学学报, 2011, 29(04): 460-464.
[2]	刘汉梅, 何瑞, 张怀渝, 黄玉碧. 拟南芥和水稻转录因子WRKY同义密码子的偏好性分析[J]. 四川农业大学学报, 2010, 28(01): 20-27.