关键词: 文昌鸡, 能量, 开产性状, NPY基因, mRNA

Abstract:

Objective to investigate the difference of NPY mRNA expression level of hypothalamus and ovary at the time before and after laying (the age of 15-week, 20-week and 25-week) and the traits in the first laying among the diets of different energy level. (Method) In this research, 600 Wenchang chickens were randomly divided into 3 groups: low energy group (GroupⅠ), control energy group (GroupⅡ) and high energy group (Group Ⅲ).There 5 replications in each group, and 40 chickens in each replication. The traits including the day in the first laying, the body weight in the first laying and the egg weight in the first laying were recorded accurately. The NPY mRNA expression of hypothalamus and ovary among different energy groups at the age of 15-week, 20-week and 25-week were analyzed by RT-PCR. The result showed that the high energy could promote the day in the first laying earlier. At the age of 15-weeks, 20-weeks and 25-weeks, the NPY mRNA expression level of hypothalamus in low energy group was all higher than that in the control and high energy groups, and the NPY mRNA expression level of ovary among the three energy groups had no significant difference. The results indicated that the change of energy level affected the NPY mRNA expression level of hypothalamus and ovary and the sex mature time of Wenchang chicken.

Key words: Wenchang chicken, Energy, Traits in the first laying, NPY gene, mRNA