四川农业大学学报 ›› 2022, Vol. 40 ›› Issue (4): 481-488.doi: 10.16036/j.issn.1000-2650.202206094

• 杂豆研究专题 • 上一篇    下一篇

重庆小豆种质资源的遗传多样性分析及白粉病抗性鉴定

龙珏臣1#, 杜成章1#, 张晓春1, 刘剑飞1, 王强1, 高飞虎1, 唐科明2, 陈红1, 张继君1,*   

  1. 1.重庆市农业科学院,重庆 401329;
    2.重庆市永川区粮油作物技术推广站,重庆 402160
  • 收稿日期:2022-06-10 出版日期:2022-08-28 发布日期:2022-08-29
  • 通讯作者: *张继君,研究员,主要从事食用豆和大豆遗传育种及栽培生理相关研究,E-mail:zhangjijun@126.com。
  • 作者简介:龙珏臣,助理研究员,E-mail:longjuechen@163.com。#为共同第一作者。
  • 基金资助:
    物种品种资源保护项目(111721301354052036); 国家现代农业产业技术体系-食用豆(CARS-08); 重庆市特色杂粮创新示范团队(CQYC201903216)

Genetic Diversity Analysis and Powdery Mildew Resistance Identification of Adzuki Bean Germplasm Resources in Chongqing

LONG Juechen1#, DU Chengzhang1#, ZHANG Xiaochun1, LIU Jianfei1, WANG Qiang1, GAO Feihu1, TANG Keming2, CHEN Hong1, ZHANG Jijun1,*   

  1. 1. Chongqing Academy of Agricultural Sciences, Chongqing 401329, China;
    2. Chongqing Yongchuan Grain and Oil Crops Technology Extension Station, Chongqing 402160, China
  • Received:2022-06-10 Online:2022-08-28 Published:2022-08-29

摘要: 【目的】 研究小豆种质资源丰富的多样性,为遗传育种研究提供重要的物质基础以及抗白粉病种质资源的筛选。【方法】 通过遗传多样性分析、主成分分析和聚类分析连续两年对参试材料开展白粉病抗性鉴定。【结果】 各性状的遗传多样性排序为:株高>成熟期>底荚高度>单株荚数>荚长>百粒重>开花期>荚宽>主茎节数>单荚粒数>分枝数>粒色>荚形>粒形>复叶叶色>生长习性>结荚习性>花色>复叶叶形>主茎色>叶脉色>叶柄色。聚类分析将59份参试材料划分为5个类群,第Ⅰ类群包含16份材料,第Ⅱ类群共包含4份材料,第Ⅲ类群共包含2份材料,第Ⅳ类群共包含31份材料,第Ⅴ类群共包含6份材料。根据主成分分析结果绘制的三维散点图中,前3个主成分将59份种质资源大致分布在两个区域,分布在图像左侧的材料多为直立型材料,而分布在右侧的材料多为半蔓生型材料。抗性鉴定结果表明,重庆地区的小豆种质资源的白粉病抗性平均处于感病水平,仍从中筛选出高抗材料6份,抗病材料4份。【结论】 通过聚类分析将重庆地区小豆种质资源被划分为5个类群,经田间诱导发病鉴定,从59份种质资源中筛选出6份高抗材料,抗病材料4份。

关键词: 小豆, 种质资源, 遗传多样性, 白粉病抗性

Abstract: 【Object】 In order to study the rich diversity of adzuki bean germplasm resources, provide important material basis for genetic and breeding research and screening of powdery mildew resistant germplasm resources, the project team collected 59 adzuki bean germplasm resources from Chongqing area in 2 years. 【Method】 Genetic diversity analysis, principal component analysis and cluster analysis were used to identify the genetic diversity, and the powdery mildew resistance of the accessions for two consecutive years. 【Result】 The genetic diversity index of each characters were ranked as: plant height> maturing days> bottom pod height> number of pods per plant> pod length> 100-seed weight> flowering days> pod width> node number of main stem> number of seeds per pod> number of branches> Seed coat color> pod shape> seed shape> trilobate leaf color> growth habit> podding habit> flower color> trilobate leaf shape> main stem color> vein color> petiole color. The 59 accessions were divided into 5 groups by cluster analysis. The Ⅰ group contained 16 accessions, the Ⅱ group contained 4 accessions, the Ⅲ group contained 2 accessions, the Ⅳ group contained 31 accessions, and the Ⅴ group contained 6 accessions. In the three-dimensional scatterplot, 59 accessions were roughly distributed in two regions by the first three principal components. Accessions on the left side of the image were mostly erect, while accessions on the right side were mostly semi-erect. The results of resistance identification showed that the average resistance of adzuki bean germplasm resources in Chongqing was susceptibility to powdery mildew, and 6 highly resistant materials and 4 resistant materials were screened out from them. 【Conclusion】 The germplasm resources of adzuki bean in Chongqing were divided into 5 groups by cluster analysis. Six high resistant and four resistant accessions were screened out from 59 germplasm resources by field induced disease identification.

Key words: adzuki bean, germplasm resource, genetic diversity, resistance to powdery mildew

中图分类号: 

  • S521