胞质甘油醛-3-磷酸脱氢酶GAPC2参与番茄果实成熟的调控

doi:10.16036/j.issn.1000-2650.202108019

摘要/Abstract

摘要： 【目的】研究异源胞质甘油醛-3-磷酸脱氢酶2（cytoplasm glyceraldehyde-3-phosphate dehydrogenase,GAPC2）对番茄果实成熟的影响。【方法】构建“红颜”草莓FaGAPC2过表达载体,采用瞬时过表达技术在樱桃番茄“罗纳”的破色期过表达FaGAPC2。【结果】 FaGAPC2的表达量为对照组的2.39倍,过表达FaGAPC2能够显著抑制番茄果皮颜色变红并显著提高其硬度（3.76N）、降低可溶性糖含量,其类胡萝卜素含量和总叶绿素含量分别是对照组的0.37倍和1.5倍。进一步通过实时荧光定量分析结果表明,过表达FaGAPC2较对照能显著降低乙烯合成途径中ACO1（12.89倍）、ACO3（3.87倍）和ERF1（3.51倍）;类胡萝卜合成途径中PSY1（12.39倍）、CYC-B（1.49倍）;果实成熟相关基因E4（4.48倍）、PG（41.35倍）和MADS转录因子RIN（6.67倍）的表达量,其中果实成熟相关基因E8、LOXB相比于对照表达量几乎为零。【结论】表明FaGAPC2作为一个负调节因子参与番茄果实成熟的调控。

关键词: 樱桃番茄, 果实成熟, 胞质甘油醛-3-磷酸脱氢酶2

Abstract: 【Objective】 This paper aims to study the effect of heterologous cytoplasmic glyceraldehyde-3-phosphate dehydrogenase 2 （GAPC2） gene on tomato fruit ripening. 【Method】 The strawberry （Fragaria × ananassa ‘Benihoppe’） FaGAPC2 overexpression vector was constructed,and transient overexpression technology was used to overexpress FaGAPC2 during the breaking period of cherry tomato （Solanum lycopersicum ‘luona’）. 【Result】 The experimental results showed that the expression level of FaGAPC2 was 2.39-fold that of the control. Overexpression of FaGAPC2 can significantly inhibit the coloring of tomato peel and the accumulation of the total soluble solids content,while increase its hardness to 3.76N. Meanwhile,the contents of carotenoid and total chlorophyll were about 0.37-fold and 1.5-fold higher than that of the control,respectively. Further real-time fluorescence quantitative analysis results showed that overexpression of FaGAPC2 can significantly reduce the expression of 1-aminocyclopropane-1-carboxylate oxidase1 （ACO1,12.89-fold）,and 1-aminocyclopropane-1-carboxylate oxidase3 （ACO3,3.87-fold）,and ethylene-responsive element factors1 （ERF1, 3.51-fold） in the ethylene synthesis pathway;phytone synthase1 （PSY1,12.39-fold）,and chromoplast-specific lycopene-β-cyclase （CYC-B,1.49-fold）in the carotenoid synthesis pathway;fruit ripening-associated genes：peptide methionine sulfoxide reductase4 （E4,4.48-fold）,polygalacturonase （PG,41.35-fold） and the MADS transcription factor ripening inhibitor （RIN,6.67-fold）. In note,the expression level of fruit ripening-related genes 1-aminocyclopropane-1-carboxylate oxidase -like （E8） and lipoxygenase （LOXB） were almost zero compared to the control. 【Conclusion】 It showed that FaGAPC2 is involved in the regulation of tomato fruit ripening as a negative regulator.

Key words: cherry tomato （Solanum lycopersicum ‘luona’）, fruit ripening, FaGAPC2

中图分类号:

S641.2

杨敏, 龙宇, 王良新, 刘晓阳, 侯国艳, 蒋语嫣, 罗娅. 胞质甘油醛-3-磷酸脱氢酶GAPC2参与番茄果实成熟的调控[J]. 四川农业大学学报, 2022, 40(2): 156-162.

YANG Min, LONG Yu, WANG Liangxin, LIU Xiaoyang, HOU Guoyan, JIANG Yuyan, LUO Ya. Study on Involvement of Cytoplasmic Glyceraldehyde-3-Phosphate Dehydrogenase GAPC2 is Involved in the Regulation of Tomato Fruit Ripening[J]. Journal of Sichuan Agricultural University, 2022, 40(2): 156-162.

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