水淹胁迫对35/66杨和NL-80351杨幼苗生理特性的影响

doi:10.16036/j.issn.1000-2650.2021.05.003

四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (5): 582-589.doi: 10.16036/j.issn.1000-2650.2021.05.003

水淹胁迫对35/66杨和NL-80351杨幼苗生理特性的影响

柳奇, 谭灵杰, 陈良华^*, 朱珍珍, 舒锟, 杨林凯, 雷浩

长江上游林业生态工程四川省重点实验室/长江上游森林资源保育与生态安全国家林业和草原局重点实验室/华西雨屏区人工林生态系统研究长期科研基地/四川农业大学生态林业研究所,成都 611130

收稿日期:2020-04-26 出版日期:2021-10-30 发布日期:2021-11-01
通讯作者: ^*陈良华,副教授,主要从事环境胁迫下林木雌雄植株间耐受性差异研究,E-mail：sicauchenlh@126.com。
作者简介:柳奇,硕士研究生。
基金资助:
四川省科技计划资助（2021YFYZ0032,2019YJ0416）; 国家级大学生创新创业训练计划资助项目（201910626050）

Effects of Waterlogging Stress on Physiological Characteristics of Poplar 35/66 and Poplar NL-80351 Seedlings

LIU Qi, TAN Lingjie, Chen Lianghua^*, ZHU Zhenzhen, SHU Kun, YANG Linkai, LEI Hao

Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province/National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River/Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base,Institute of Ecology/Forestry,Sichuan Agricultural University,Chengdu 611130,China

Received:2020-04-26 Online:2021-10-30 Published:2021-11-01

摘要/Abstract

摘要： 【目的】比较两个生长性状优良、分布范围广泛的杨树品种“35杨（Populus deltoides CL‘35/66’）”和“NL-80351杨（P. deltoides cv. NL-80351）”对水淹胁迫适应能力的差异并分析相关的生理机制。【方法】采用盆栽控制实验的方法,以35杨和NL-80351杨为材料,研究水淹处理对两个品种美洲黑杨幼苗生物量积累与分配、抗氧化酶活性、非结构性碳水化合物（NSC）分配和氮代谢酶活性等方面的影响。【结果】 ①水淹胁迫可以显著降低两个品种杨树的根系生物量,对NL-80351杨的影响更大。②水淹胁迫显著提高了35杨叶片过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性,对丙二醛（MDA）含量无显著影响;NL-80351杨的两种酶活性在水淹条件下均无显著变化,MDA含量显著增加。③水淹胁迫条件下,35杨叶片、茎的淀粉含量及茎的葡萄糖含量显著增加,NL-80351杨叶片淀粉和蔗糖含量增加,茎的葡萄糖、蔗糖和淀粉含量降低,两者不同根级的NSC没有表现出一致的规律。④水淹胁迫抑制了NL-80351杨叶片的硝酸还原酶（NR）、谷氨酰胺合成酶（GS）和谷氨酸合酶（GOGAT）的活性,提高了谷氨酸脱氢酶（GDH）的活性,引起铵态氮含量的增加,但对35杨氮代谢过程无显著影响。【结论】与NL-80351相比,35杨具有更强的维持根系生长的能力,表现出较强的抗氧化胁迫和维持碳分配平衡、氮同化的能力,对水淹胁迫有更强的生理适应性。

关键词: 美洲黑杨, 水淹胁迫, 非结构性碳水化合物, 氮代谢

Abstract: 【Objective】 To compare the adaptability to flooding stress of two poplar varieties, ‘Poplar 35 (Populus deltoides CL‘35/66’)’and ‘Poplar NL-80351 (P. deltoides cv. NL-80351)’, with good growth traits and wide distribution, and to analyze the related physiological mechanism. 【Method】 A pot control experiment was conducted with Poplar 35 and Poplar NL-80351 as materials. The effects of one-month waterlogging stress on biomass accumulation and allocation, antioxidant enzyme activity, non-structural carbohydrate (NSC) allocation and nitrogen metabolism related enzyme activity of two varieties of P. deltodies seedlings cultivars were studied. 【Result】 ①Waterlogging stress significantly reduced root biomass of two poplar varieties, especially Poplar NL-80351.②Waterlogging stress significantly increased the activities of peroxidase (POD) and superoxide dismutase (SOD) in leaves of Poplar 35, but had no significant effect on the content of malondialdehyde (MDA). However, there were no significant changes in the activities of the two enzymes in Poplar NL-80351, while the MDA content increased significantly in Poplar NL-80351. ③The starch content and glucose content in leaves and stems of Poplar 35 were significantly increased under waterlogging stress, the starch and sucrose contents in leaves of Poplar NL-80351 were increased, and the glucose, sucrose and starch contents in stems were decreased, but there was no consistent rule in NSC of different root levels of two varieties of poplar. ④The activities of nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) in leaves of Poplar NL-80351 were inhibited by flooding stress, and the content of ammonium nitrogen was increased, but the effect on nitrogen metabolism was not significant to Poplar 35. 【Conclusion】 In conclusion, compared with Poplar NL-80351, Poplar 35 showed stronger ability to maintain root growth, stronger ability to maintain carbon distribution balance and nitrogen assimilation, and stronger physiological adaptability to flooding stress.

Key words: P. deltodies, waterlogging stress, non-structural carbohydrates, nitrogen metabolism

中图分类号:

S718.43

柳奇, 谭灵杰, 陈良华, 朱珍珍, 舒锟, 杨林凯, 雷浩. 水淹胁迫对35/66杨和NL-80351杨幼苗生理特性的影响[J]. 四川农业大学学报, 2021, 39(5): 582-589.

LIU Qi, TAN Lingjie, Chen Lianghua, ZHU Zhenzhen, SHU Kun, YANG Linkai, LEI Hao. Effects of Waterlogging Stress on Physiological Characteristics of Poplar 35/66 and Poplar NL-80351 Seedlings[J]. Journal of Sichuan Agricultural University, 2021, 39(5): 582-589.

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水淹胁迫对35/66杨和NL-80351杨幼苗生理特性的影响

Effects of Waterlogging Stress on Physiological Characteristics of Poplar 35/66 and Poplar NL-80351 Seedlings

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