一个小麦中水稻根系直系同源基因的分子鉴定及表达分析

摘要/Abstract

摘要： 【目的】分析水稻中OsRAA1在小麦中的同源基因TaRAA1的结构、进化、启动子调控区域、组织特异性表达以及非生物胁迫表达等,初步探索其在小麦中的生物学功能,为进一步遗传转化研究其功能提供指导。【方法】利用在线数据库获得TaRAA1的基因编码序列和蛋白结构, 进一步分析其进化和表达模式,最后在“中国春”中进行RT-qPCR验证该基因的组织特异性表达以及非生物胁迫表达。【结果】TaRAA1被定位到小麦第三同源染色体（3A、3B和3D）,在所有分析物种中均无内含子,其基因结构具有高度保守性。在所研究的物种中除在水稻中检测到一个低复杂区域外,其他物种中均未检测到任何功能域。组织特异性表达分析表明,TaRAA1基因主要在小麦的根部大量表达。公共数据库和RT-qPCR分析结果都表明小麦TaRAA1在高温、高盐、干旱处理下,表达量受到正向调控;而在低温和PEG处理下,表达受到负向调控。【结论】TaRAA1基因可能在小麦受到非生物逆境胁迫时发挥着重要作用。

关键词: 普通小麦, TaRAA1, 根发育, 非生物胁迫, 表达分析

Abstract: 【Objective】 The structure, evolution, promoter regulatory region, tissue-specific expression and abiotic stress expression of the root-related ortholog of rice TaRAA1 in wheat were analyzed to explore its possible biological roles aiming at laying a foundation for further studying its function through genetic transformation. 【Method】 The gene coding sequence and protein structure of TaRAA1 were obtained using a public database. The evolution, expression pattern, tissue-specific expression, and abiotic stress expression were further analyzed using public data combined with RT-qPCR in ‘Chinese Spring’ wheat.【Result】 TaRAA1 was mapped to the third chromosome (3A, 3B and 3D) of wheat. Its gene structure was highly conserved among various species. In the researched species， except for a low complex region detected in rice， no functional domain was detected in other species. The analysis of tissue-specific expression showed that the TaRAA1 gene was mainly expressed in wheat roots. The expression results from the public database and RT-qPCR showed that the expression of TaRAA1 was positively up-regulated under high temperature， high salt and drought， but down-regulated under low temperature and PEG. 【Conclusion】 TaRAA1 may play an important role in wheat under abiotic stress providing a clue for further analysis of its function through genetic transformation.

Key words: common wheat, TaRAA1, root development, abiotic stress, expression analysis

中图分类号:

S512.1

张雪梅, 李婷, 涂洋, 兰秀锦, 龚江洪, 唐力为, 马建. 一个小麦中水稻根系直系同源基因的分子鉴定及表达分析[J]. 四川农业大学学报, 2020, 38(06): 645-653.

ZHANG Xuemei, LI Ting, TU Yang, LAN Xiujin, GONG Jianghong, TANG Liwei, MA Jian. Molecular Identification and Expression Analysis of A Root-Related Ortholog of Rice in Common Wheat[J]. Journal of Sichuan Agricultural University, 2020, 38(06): 645-653.

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