关键词: 泥鳅, 甘露寡糖, 生产性能, 肠道菌群, 非特异性免疫

Abstract: 【Objective】 The experiment was conducted to study the effects of mannose oligosaccharide on productive performance, intestinal flora and non-specific immune function of Misgurnus anguillicaudatus. 【Method】 360 healthy M. anguillicaudatus with an average body weight of (3.27 ± 0.05) g were selected, and randomly divided into 6 groups with 3 replicates and 20 individuals per replicate. 0 (control group), 100, 200, 400, 600 and 800 mg/kg of mannose oligosaccharide were added to the basal diet, respectively. Various indexes of production performance, intestinal flora and non-specific immune function were detected after 70-day breeding. 【Result】 ① The specific growth rate, condition factor, intestinal weight indexes and intestinal length indexes in 200 mg/kg and 400 mg/kg groups were significantly higher while feed coefficient s were significantly lower than those in control group (P<0.05); ②The amount of Lactobacillus and Bifidobacterium in 200 mg/kg and 400 mg/kg groups were significantly higher but the amount of Escherichia coil were significantly lower than those of control group (P<0.05); ③Compared with control group, SOD and CAT activities in plasma, hepatopancreas and LZM in plasma of 200 mg/kg and 400 mg/kg groups were significantly increased (P<0.05). 【Conclusion】 Diet with 200-400 mg/kg mannose oligosaccharide can significantly promote intestinal development, optimize micro-ecology circumstances, and improve non-specific immunity function and growth performance of M. anguillicaudatus.

Key words: Misgurnus anguillicaudatus, mannose oligosaccharide, production performance, intestinal flora, non-specific immunity