›› 2018, Vol. 36 ›› Issue (04): 542-548.

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### 饲粮NDF水平对山羊营养物质表观消化率及瘤胃古菌结构与组成的影响研究

1. 四川农业大学动物营养研究所
• 收稿日期:2017-11-06 修回日期:2018-07-18 出版日期:2018-08-31 发布日期:2018-11-29
• 通讯作者: 王立志 E-mail:wanglizhi08@aliyun.com
• 基金资助:
“十三五”国家重点研发计划项目“畜禽现代化饲养关键技术研发”

### Effects of dietary NDF levels on apparent digestibility of nutrients and the composition of rumen archaea in goats

• Received:2017-11-06 Revised:2018-07-18 Online:2018-08-31 Published:2018-11-29

Abstract: Abstract: (Objective) This study was conducted to examine the effects of dietary NDF levels on apparent digestibility of nutrients and the composition of rumen archaea in goats using metabolic trial and high throughput sequencing technique. (Method) Six eight-month-old healthy male goats were chosen for the 3×3 Latin square experiments. Three experimental treatments (low dietary NDF level group (LN), middle dietary NDF level group (MN), and high dietary NDF level group (HN)) were set for metabolic trial. The whole experiment was divided into three phases, and rumen contents of each goat were collected after each phase. The total DNA of rumen microorganism in the rumen content was extracted. Primers specific for archaea were used to amplified the V3-V4 high variable regions of 16S rRNA gene. Bioinformatics analysis of PCR products was performed by high-throughput sequencing using Illumina platform. (Result) The results showed that: 1）There were no significant differences in the apparent digestibility of DM, OM and CP between the three groups (P>0.05) . The apparent digestibility of EE in LN group was significantly higher than that in MN group and HN group (P<0.05).The apparent digestibility of NDF in HN group was significantly higher than that in LN group and MN group (P<0.05). 2）The Chao1, Shannon and Observed species indices of LN group were significantly higher than those of the other two groups (P<0.05), but no significant difference between the other two groups (P>0.05). 3) At phylum level, the relative abundance of all taxonomy in the three treatment groups was not significant different (P>0.05), and the dominant phylum was Euryarchaeota.4）At genus level, Methanobrevibacter, Candidatus_Methanomethylophilus and Candidatus_Methanoplasma were the dominant genera of the three treatment groups. The relative abundance of Methanobrevibacter in LN group was remarkable significantly lower than that in the other two groups (P<0.01), but the relative abundance of Candidatus_Methanomethylophilus and Candidatus_Methanoplasma was significantly higher than that of the other two groups (P<0.05), these archaea were no significant difference between the other two groups. The other archaeal genera were no significant difference between the three treatments (P>0.05). Conclusion: When dietary NDF levels varied from 35% to 45%, the apparent digestibility of EE and NDF in diets was significantly affected in goats, and the relative abundance of major archaea such as Methanobrevibacter in rumen was significantly affected.