关键词: 亚硒酸钠, 黄曲霉毒素, 胸腺, 凋亡, 死亡受体通路

Abstract: This experiment was conducted to investigate the protective mechanism of selenium on AFB1-induced thymocytes apoptosis. One hundred and ninety COBB chickens were randomly divided into four groups, and fed on control diet, aflatoxin B1 diet, +Se diet and AFB1+Se diet respectively for 21 days. Thymus was sampled for detecting the relative weight, T-cell subsets, percentage of apoptotic cells and relative mRNA expression of genes in the death receptor pathway at 7, 14 and 21 days of age. Our results showed that compared with the AFB1 group, Se could increase the relative thymus weight, alleviate histological lesions, reduce the incidence of apoptotic thymocytes, decrease the relative mRNA expression of JNK, TNF-α, caspase-8, caspase-3 and caspase-9, and increase the relative mRNA expression of Bid and Bcl-2. It was speculated that Se could alleviate the AFB1-induced lesions in thymus partly via the break in the following two pathways, Fas-FasL-FADD-caspase8-caspase3 pathway and Fas-ASK1-JNK-Bcl-2 pathway.

Key words: sodium selenite, Aflatoxins, thymus, apoptosis, death receptor pathway