关键词: 关键词：副猪嗜血杆菌, 二价灭活苗, 抗体滴度, 免疫保护效力。

Abstract: Abstract: This study developed a new kind of bivalent inactivated vaccine aiming at controlling Haemophilus parasuis (HPs) disease, and the vaccine’s immune effect was evaluated. The most prevalent serotype 5 strain MC203 and serotype 13 strain XJ103 in Sichuan province are selected, which was followed by inactivated with 0.2% formaldehyde, and then washed with PBS, after which, the MC-203, XJ-103 and alhydrogel adjuvant were mixed with a scale of 1:1:2 to make HPs bivalent vaccine. The specific antibody against HPs were detected through ELISA and safety evaluated in animal challenge experiments. The antibody titer of the bivalent inactivated vaccine against HPs were evaluated in piglets. In order to compare the immune efficacy among the bivalent inactivated vaccine, imported inactivated vaccine and domestic inactivated vaccine, piglets were immunized with the three kinds of vaccines and the immune efficacy was evaluated by results of immunized protection. The results were as follows: ①HPs was inactivated completely with 0.2% formaldehyde after incubation for 16 h at 37℃. ②HPs inactivated vaccine has a high security in piglets and without undesirable reaction. ③The specific antibody against HPs could be detected at the 14 day and lasted at least for 196 days after vaccination. ④Comparison of the immunity effectiveness of the inactivated vaccine demonstrated that the inactivated vaccines, imported inactivated vaccine and domestic inactivated vaccine immune have a protection of 80%, 80% and 60%, respectively. Through above, we got the conclusion that inactivated HPs vaccine in this study has an expected protection in piglets against predominant serotype of HPs.

Key words: Key words: Haemophilus parasuis, bivalent inactivated vaccine, antibody titer, immune protection force.