猪乙型脑炎病毒基因I型弱毒株免疫小鼠后诱导INF-γ的能力

›› 2018, Vol. 36 ›› Issue (02): 233-237.

猪乙型脑炎病毒基因I型弱毒株免疫小鼠后诱导INF-γ的能力

王侨¹,柴春霞¹,²,冯瑶¹,³,曹钰莹¹,¹,邓李霜¹,¹,黄小波⁴,曹三杰²,文翼平¹,¹,赵勤⁵,伍锐¹,⁶

1. 四川省成都市温江区四川农业大学
2. 四川农业大学动物医学院
3. 四川农业大学
4.
5. 四川农业大学食品学院
6. 四川省成都市温江区四川农业大学惠民路211号

收稿日期:2017-04-07 修回日期:2018-03-13 出版日期:2018-04-23 发布日期:2018-11-29
通讯作者: 伍锐 E-mail:WURUI1977-@163.COM
基金资助:
四川农业大学学科建设双支计划;国家重点研发计划

The ability of genotype I swine Japanese encephalitislive- attenuate strain to induce INF-gamma in mice after immunization

Received:2017-04-07 Revised:2018-03-13 Online:2018-04-23 Published:2018-11-29

摘要/Abstract

摘要： [目的]评估基因I型猪乙型脑炎病毒弱毒株（SCYA201201株）作为候选疫苗诱发机体产生细胞免疫的能力。[方法]将基因I型猪乙型脑炎弱毒株（SCYA201201株F122代次）腹腔免疫小鼠后使用ELISA检测试剂盒测定早期的INF-γ水平，之后测定INF-γ在小鼠体内的持续时间。[结果]结果显示：猪乙型脑炎SCYA201201弱毒株（F122代次）经腹腔免疫小鼠后，小鼠血清中的INF-γ在免疫后6h就可上升到一个较高水平，且INF-γ在血清和小鼠脾脏淋巴细胞中均可持续14d以上。[结论]猪乙型脑炎SCYA201201弱毒株（F122代次）免疫小鼠后能够在早期诱发INF-γ产生，并且能够维持14天以上。有作为候选疫苗的潜力。

关键词: 基因I型, 猪乙型脑炎病毒, 弱毒株, 候选疫苗, INF-γ

Abstract: [Objective] To evaluate Japanese encephalitis virus SCYA201201 strain of the genotype I induce cell immunity as vaccines candidate in mouse model. [Method] This research using ELISA kit the INF-γ detect the INF-γ levels of mice serum after immune with genotype I of Japanese encephalitis live-attenuated strain (SCYA201201 strain). [Results] The results showed that Japanese encephalitis live-attenuated SCYA201201 strain (F122 generation) of mice can induced INF- gamma level to a higher level when post immunization 6h in mice serum, and INF- gamma in serum and lymphocytes were sustained for more than 14days. [Conclusion] The study proved that the live-attenuated SCYA201201 strain can induce the production of INF- gamma in early immunity stage, and it can be maintained for a long time. There is potential for a candidate vaccine。

Key words: genotype I, JEV, Live attenuated vaccine, Candidate vaccine, INF-γ

王侨柴春霞冯瑶曹钰莹邓李霜黄小波曹三杰文翼平赵勤伍锐. 猪乙型脑炎病毒基因I型弱毒株免疫小鼠后诱导INF-γ的能力[J]. , 2018, 36(02): 233-237.