关键词: 关键词： 川红柴1号, 中柱鞘, 生长素, 侧根发育

Abstract: [Abstract] 【Objective】 To explore the lateral root development process and influence factors in Bupleurum scorzonerifolium Willd..【Methods】The dynamic growth of lateral root primordium development was observed by applying stereoscopic microscope and light microscopy. In addition, treatments with different concentrations IAA, TIBA, Ca2+, and EDTA were set up to analyse their influence on lateral root development. 【Results】 The lateral root primordium of “Chuanhongchai No.1” (Bupleurum scorzonerifolium Willd.) originated the pericycle cells. 0.01 μmol/L IAA and 1 μmol/L Ca2+ greatly promoted the development of lateral root, but its high concentrations inhibited lateral root emergence. 0.1 μmol/L TIBA also greatly promoted the development of lateral root, but its high concentrations inhibited lateral root emergence. The formation of lateral roots mostly inhibited by EDTA. 【Conclusion】This research method is very important to medicinal plant cultivation and breeding.

Key words: Key words: Chuanhongchai No.1, The pericycle, Auxin, Lateral root development