南江黄羊ARHGAP11A基因SNPs筛选及其与生长性状的关联分析

摘要/Abstract

摘要： （目的）为探讨ARHGAP11A基因与家畜生长性状的关联。（方法）试验以南江黄羊 (母羊，n=243)为试验材料，采用PCR产物直接测序筛选ARHGAP11A基因的SNP位点并分析其与性状之间的关联。（结果）南江黄羊群体中共发现ARHGAP11A基因16个SNPs位点。g.3483A>G和g.3621G>A的纯合突变具有增加南江黄羊体重（2月龄、6月龄和12月龄）以及胸围（6月龄）指标的趋势（P>0.05），而在g.3525A>G位点AG型羊初生重、12月龄体重和12月龄胸围显著高于GG型个体（P<0.05）。AAGT单倍型山羊体重体尺均高于AGGT和GGAT个体（P>0.05）。（结论）南江黄羊母羊群体中ARHGAP11A基因突变与生长发育密切相关，进一步验证其功能后在选育中可考虑其作为辅助选择标记。

关键词: 南江黄羊, ARHGAP11A基因, 单核苷酸多态性位点（SNP）, 生长性能, 关联分析

Abstract: [Objective] The objective of this study was to investigate the linkage between ARHGAP11A gene and the growth traits of livestock. [Method] Using Nanjiang Yellow goats (female, n=243) as experimental materials,then detected the single nucleotide polymorphisms (SNPs) in ARHGAP11A gene by direct sequencing and analyzed the potential effects of these SNPs with the growth traits. [Results] A total of novel 16 SNPs of ARHGAP11A gene were identified in Nanjiang Yellow goats. In addition, homozygous mutations at g.3483A>G and g.3621G>A were related with increased body weight (2 months, 6 months and 12 months) and chest circumference (6 months) (P>0.05). Meanwhile at g.3525A>G, individuals with AG genotype were significantly larger than those of GG-genotype goats, especially body weight at birth and 12 month and chest circumference at 12 month (P<0.05). Furthermore, comparing with AGGT and GGAT goats, AAGT-haplotype individuals tended to be dominant at body mass and size (P>0.05). [Conclusion] These results preliminarily elucidate that polymorphisms of ARHGAP11A gene are closely related to the development of female Nanjiang Yellow goats, which could be used as molecular marker in breeding when the effects of these mutations are further proved.

Key words: Nanjiang Yellow Goat, ARHGAP11A, single nucleotide polymorphism (SNP), growth trait, correlation analysis

王嘉欣张德鹏李利张红平. 南江黄羊ARHGAP11A基因SNPs筛选及其与生长性状的关联分析[J]. , 2018, 36(01): 91-99.

参考文献

[1] Jie X, Zhou X, Wang J, et al. RhoGAPs attenuate cell proliferation by direct interaction with p53 tetramerization domain.[J]. Cell Reports, 2013,3(5):1526. [2] Amin E, Jaiswal M, Derewenda U, et al. Deciphering the molecular and functional basis of RhoGAP family proteins: A systematic approach towards selective inactivation of Rho family proteins[J]. Journal of Biological Chemistry, 2016,291(39):M116-M736967. [3] Jacobs T, Hall C. Rho GAPs — Regulators of Rho GTPases and More[M]. Springer Netherlands, 2005. [4] Romanov V S, Pospelov V A, Pospelova T V. Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis[J]. Biochemistry Biokhimii?a, 2012,77(6):575-584. [5] Kagawa Y, Matsumoto S, Kamioka Y, et al. Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo[J]. Plos One, 2013,8(12):e83629. [6] Zuo Y, Oh W, Frost J A. Controlling the switches: Rho GTPase regulation during animal cell mitosis[J]. Cellular Signalling, 2014,26(12):2998-3006. [7] 张红平, 王维春, 熊朝瑞, 等. 南江黄羊的种质特性[J]. 中国草食动物科学, 2004(z1):113-114. [8] 陈利. 南江黄羊和波尔山羊骨骼肌卫星细胞增殖分化能力的比较[D]. 四川农业大学, 2014. [9] Hasty P, Bradley A, Morris J H, et al. Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene.[J]. Nature, 1993,364(6437):501. [10] J P M, N O E. Potthoff M J, Olson E N. MEF2: a central regulator of diverse developmental programs[J]. Development, 2007,134(23):4131-4140. [11] Chang M T, Cheng Y S, Huang M C. A novel non-synonymous SNP of the COLX gene and its association with duck reproductive traits[J]. Molecular & Cellular Probes, 2012,26(5):204-207. [12] Fan B, Du Z Q, Daniellem G, et al. Development and application of high-density SNP arrays in genomic studies of domestic animals.[J]. Asian Australasian Journal of Animal Sciences, 2010,23(7):833-847. [13] Vignal A, Milan D, Sancristobal M, et al. A review on SNP and other types of molecular markers and their use in animal genetics.[J]. Genetics Selection Evolution, 2002,34(3):1-31. [14] Hadjipavlou G, Matika O, Clop A, et al. Two single nucleotide polymorphisms in the myostatin ( GDF8 ) gene have significant association with muscle depth of commercial Charollais sheep[J]. Animal Genetics, 2008,39(4):346-353. [15] Hickford J G H, Forrest R H, Zhou H, et al. Polymorphisms in the ovine myostatin gene ( MSTN ) and their association with growth and carcass traits in New Zealand Romney sheep[J]. Animal Genetics, 2010,41(1):64-72. [16] Mignone F, Gissi C, Liuni S, et al. Untranslated regions of mRNAs[J]. Genome Biology, 2002,3(3):s1-s4. [17] Ma Y, Li R R, Hou F, et al. Comparative Mapping and 3'UTR SNP Detection of ANGPTL4 Gene in Beef Cattle[J]. Journal of Animal & Veterinary Advances, 2011,10(13):1649-1655. [18] Elbein S C, Wang X, Karim M A, et al. Role of a proline insertion in the insulin promoter factor 1 (IPF1) gene in African Americans with type 2 diabetes.[J]. Diabetes, 2006,55(10):2909-2914. [19] Jacquemin P, Lannoy V J, Rousseau G G, et al. OC-2, a novel mammalian member of the ONECUT class of homeodomain transcription factors whose function in liver partially overlaps with that of hepatocyte nuclear factor-6[J]. Journal of Biological Chemistry, 1999,274(5):2665.