›› 2017, Vol. 35 ›› Issue (04): 594-598.

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  1. 上海交通大学
  • 收稿日期:2017-05-18 修回日期:2017-09-13 出版日期:2017-12-30 发布日期:2018-11-29
  • 通讯作者: 徐建雄 E-mail:jxxu1962@sjtu.edu.cn

Effects of micro-derived antioxidants on antioxidant capacity and litter capacity in high fat induced pregnant rats

  • Received:2017-05-18 Revised:2017-09-13 Online:2017-12-30 Published:2018-11-29

摘要: 摘 要:【目的】试验旨在探讨微生物源性抗氧化剂对高脂诱导的氧化应激孕鼠抗氧化能力和产仔能力的影响。【方法】选择18只清洁级体重相近的SD孕鼠,随机分成3组,每组6只(重复),分别为基础日粮组、高脂日粮组和高脂日粮添加2%微生物源性抗氧化剂组(修复组)。试验从妊娠第1天始至分娩结束,记录相关生产性能指标,收集母鼠分娩时血液分离血浆,测定抗氧化指标。【结果】与基础组相比,高脂组母鼠末重和日增重无显著变化(P>0.05),日平均采食量显著降低(P<0.05),产仔数、初生重和初生窝重没有显著变化(P>0.05);与高脂组相比,修复组母鼠末重、日平均采食量和日增重极显著降低(P<0.01),产仔数和初生窝重无显著变化(P>0.05),初生重显著降低(P<0.05)。与基础组相比,高脂组母鼠血浆中GSH-Px、抑制羟基自由基能力、SOD和CAT活性显著降低(P<0.05)、MDA和蛋白羰基含量显著提高(P<0.05),血浆中T-AOC降低极显著(P<0.01);与高脂组相比,修复组极显著降低母鼠血浆中MDA和蛋白羰基含量(P<0.01),极显著地提高母鼠血浆T-AOC、CAT和GSH-Px活性(P<0.01),显著地提高了SOD活性和抑制羟基自由基能力(P<0.05)。【结论】高脂日粮加剧了妊娠母鼠的氧化应激,微生物源性抗氧化剂提高了高脂诱导的妊娠母鼠的抗氧化能力,缓解机体的氧化损伤,促进胎儿健康发育,然而添加后是否适口性降低影响了采食量需要做进一步的研究。

关键词: 关键词:微生物源性抗氧化剂, 抗氧化能力, 氧化损伤, 妊娠, 大鼠

Abstract: Abstract:【Objective】The aim of this study was to investigate the effects of micro-derived antioxidants(MA) on the antioxidant capacity and reproductive performance of high fat diet induced oxidative stress in pregnant rats. 【Method】Eighteen pregnant SD rats with similar body weight were randomly divided into 3 groups (6 rats in each group), the basal diet group(B), high fat diet group(HF) and high fat diet with 2% MA group (repair group, R). The experiment was started from the first day of pregnancy to the end of delivery, and the data of reproductive performance were recorded. Maternal blood were collected after delivery, and plasma were separated to determine antioxidant indexes. 【Results】Compare with the B group, there was no significant difference of weight and daily gain(P >0.05) in HF group .The average daily feed intake in the HF group was significantly lower than that in the B group (P <0.05), but the litter size, the litter weight and the primary weight did not showed significant difference (P >0.05). Compared with the HF group, the litter weight, average daily feed intake and daily gain were extremely decreased in the R group (P <0.01). But no significant difference of the litter size and litter weight were observed (P >0.05). Compared with the B group, HF group significantly decreased the activities of GSH-Px, the inhibitory hydroxyl ability (IHA),SOD and CAT(P <0.05), and the contents of MDA and protein carbonyl were significantly increased (P <0.05), the T-AOC of in plasma was extremely decreased (P <0.01); Compared with the HF group, the R group extremely increased the activity of T-AOC, CAT and GSH-Px (P<0.01) in plasma, significantly increased the activity of SOD and IHA(P <0.05). 【Conclusions】MA improve the antioxidant capacity reversed the high fat induced oxidative damage, promote fetal development, however, whether the reduction of feed intake caused by palatability needs to be further studied..

Key words: Key words: micro-derived antioxidants, antioxidant capacity, oxidative damage, pregnant, rats


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