关键词: 匙吻鲟, 表面葡萄球菌, 分离鉴定, 药敏试验

Abstract: [Objective] This study was carried out to isolation, identification and drug susceptibility test of pathogenic bacterium from Polyodon spathula, for providing a reference for prevention and treatment of hemorrhagic disease of Polyodon spathula. [Methods] Bacteria isolation from diseased paddlefish, bacteria morphological observation, physiological and biochemistry reaction by biology automatic microbiological assay system, sequencing and analysis of 16S rDNA, PCR primers specificity testisolation, and identification of pathogenic bacterium, finally the drug susceptibility test were performed with Kirby-Bauer disc diffusion method. [Result] One strain Staphylococcus epidermidis from tissues of diseased and dying Polyodon spathula, named Pol15001, identified based on the bacterial culture, The results showed Staphylococcus epidermidis were resistant to 7 kinds of drugs including selectrin trime-sulf, clindamycin, ampicillin, etc. While sensitive to ceftriaxone, mefoxin, chloroamphenicol and cef otaxime. [Conclusion] The Staphylococcus epidermidis was a new pathogenic bacteria of Polyodon spathula. Improving the aquaculture water quality and taking ceftriaxone, mefoxin, chloroamphenicol and cefo-taxime are effective preventions and controls in aquaculture.

Key words: Polyodon spathula, Staphylococcus epidermidis, isolation and identification, drug susceptibility test