四川农业大学学报 ›› 2015, Vol. 33 ›› Issue (01): 51-56.doi: 10.16036/j.issn.1000-2650.2015.01.009

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黑曲霉固态发酵甘薯渣条件优化及发酵对甘薯渣营养品质的影响

赵华, 王雪涛, 汤加勇, 汤小朋, 贾刚, 刘光芒, 陈小玲, 王康宁   

  1. 四川农业大学动物营养研究所, 四川 温江 611130
  • 收稿日期:2014-11-25 出版日期:2015-03-31 发布日期:2017-02-28
  • 通讯作者: 赵华,博士,副研究员,硕士生导师,主要研究领域为营养与分子生物学,E-mail:zhua666@126.com。 E-mail:zhua666@126.com
  • 作者简介:王雪涛,硕士研究生。
  • 基金资助:
    四川农业大学双支计划;四川科创饲料产业技术研究院基金(NO:2013NZ0054)

Solid-state Fermentation Parameters Optimization and Nutritional Improvement of Sweet Potato Residue with Aspergillus niger

ZHAO Hua, WANG Xue-tao, TANG Jiao-yong, TANG Xiao-peng, JIA Gang, LIU Guang-mang, CHEN Xiao-ling, WANG Kang-ning   

  1. Animal Nutrition Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China
  • Received:2014-11-25 Online:2015-03-31 Published:2017-02-28

摘要: [目的] 研究黑曲霉固态发酵对甘薯渣营养价值的影响,并探讨其最佳发酵工艺参数。[方法] 采用单因素试验设计,对黑曲霉3.287固态发酵甘薯渣的发酵参数如接种量、发酵时间、料水比和温度等进行优化,在此基础上,进一步采用正交试验设计,筛选出最佳发酵工艺参数,考察在最佳发酵参数条件下黑曲霉固态发酵对甘薯渣营养品质的影响。[结果] 结果表明:1每克发酵原料接种黑曲霉孢子1×106个,发酵时间4d,料水比1:1.3,发酵温度38℃条件下发酵效果最好。2在黑曲霉优化参数下发酵,以干物质为基础,粗蛋白含量从6.37%提高到9.14%;粗脂肪从2.71%提高到4.75%;发酵后还原糖含量达到从4.42%提高到7.34%,羧甲基纤维素酶活、滤纸酶活、β-葡萄糖苷酶活和淀粉酶活分别为:3.12、2.45、2.13和3.02 U。[结论] 农副产品甘薯渣经过黑曲霉固态发酵可以有效改善其营养品质。

关键词: 黑曲霉, 固态发酵, 甘薯渣, 营养价值

Abstract: [Objective] This experiment was conducted to investigate the nutritional improvement of sweet potato residue by solid-state fermentation (SSF)using Aspergillus niger and the optimal fermentation parameters were investigated. [Method] Single factor experiment design was adopted to investigate the effects of inoculation size, fermentation time, moisture and temperature on nutritional improvement of sweet potato residue and a orthogonal experiment design was further used to optimize the fermentation parameters described above. [Results] The results showed as following:① Inoculated 1×106 germ per gram fermentation materials with Aspergillus niger strain 3.287, controlled the ratio of substrate vs water ratio to 1:1.3and fermented at 38℃ for 4 days get a best nutritional improvement of sweet potato residue. ② Fermented sweet potato residue under the optimized condition described above and calculated base on dry matter, crude protein was increased from 6.67% to 9.14% (P<0.05);crude fat was increased from 2.71% to 4.75% (P<0.05);the reducing sugar increased from 4.42% to 7.34%. Moreover, the enzyme activity of CMCase, FPA, β-glucosidase and amylase reached to 3.12, 2.45, 2.13 and 3.02 U per gram dry matter, respectively. [Conclusion] The SSF with Aspergillus niger offers an effective alternative to improving the quality of unconventional feed sources such as sweet potato residue.

Key words: Aspergillus niger, solid-state fermentation, sweet potato residue, nutritional value

中图分类号: 

  • S816.6