优质肉鸡肌苷酸与肌内脂肪含量相关基因聚合效应研究

四川农业大学学报

优质肉鸡肌苷酸与肌内脂肪含量相关基因聚合效应研究

1四川省畜牧科学研究院，四川，成都，610066;2 四川大恒家禽育种有限公司，四川，成都，610066;3 西南名族大学生命科学与技术学院，四川，成都，610041

收稿日期:2013-09-11 出版日期:2013-09-27 发布日期:2013-01-09

Study on Inosine Monophosphate and Intramuscular Fat Related Multiple Gene Pyramiding Effect in Meat Quality Broiler

Received:2013-09-11 Online:2013-09-27 Published:2013-01-09

摘要/Abstract

摘要：

[目的] 探讨多基因聚合在优质肉鸡分子育种中的可行性。[方法]运用PCR-SSCP和测序技术检测ADSL、GARS-AIRS-GART和A-FABP 3个基因的优势纯合基因型（CCAAMM）。[结果]研究结果显示，3个基因的优势纯合基因型聚合后代完全也是优势纯合基因型（CCAAMM）, 在后代个体中优势纯合基因型个体的肌苷酸含量显著高于其它非完全有利聚合基因型个体（P﹤0.05）。除了CCBBMM型，CCAAMM型的肌内脂肪含量显著高于其它聚合基因型（P<0.05）。[结论]由此，推测CCAAMM聚合基因型可作为大恒优质肉鸡肉质性状有效的候选标记。

关键词: 鸡; ADSL基因；GARS-AIRS-GART基因；A-FABP基因；基因聚合

Abstract:

[Objective] The aim of this study is to detect the feasibility of multiple gene pyramiding breeding in well-quality Broiler. [Method] Single nucleotide polymorphisms (SNPs) of candidate genes in Daheng well-quality Broiler with S01 line (parent strain) and D groups (pyramiding offspring) were detected by PCR-SSCP and sequencing. [Results] The result showed that dominant homozygotes genotype (CCAAMM) in their F1 offspring was also CCAAMM genotype. The inosine monophosphate content of the dominant homozygotes genotype (CCAAMM) was higher than other genotypes (p<0.05), the intramuscular fat of the dominant homozygotes genotype (CCAAMM) was higher than other genotypes (p<0.05) except for the CCBBMM genotype. [Conclusion] We speculated that the homozygote genotype (CCAAMM) was a major marker affecting chicken meat quality traits.

Key words: Chicken; ADSL gene; GARS-AIRS-GART gene; A-FABP gene; Multiple gene pyramiding

张增荣1,杜华锐1, 金红3, 李晴云2,杨朝武1,李雯2, 邱莫寒1,刘岚2,宋小燕2,蒋小松1*. 优质肉鸡肌苷酸与肌内脂肪含量相关基因聚合效应研究[J]. 四川农业大学学报, 2013, 31(03): 340-344.